Laboratory of Structural Biology: Bochtler Laboratory

  • 2017
  • Bochtler M, Kolano A, Xu G-L. DNA demethylation pathways: Additional players and regulators. 2017. Bioessays, 39(1):1-13 . details
  • 2016
  • Bochtler M. Indirect DNA Sequence Readout by LAGLIDADG Homing Endonucleases. 2016. Structure, 24(6):839-40 . details
  • Szychowska M, Siwek W, Pawolski D, Kazrani AA, Pyrc K, Bochtler M. Type III CRISPR complexes from Thermus thermophilus. 2016. Acta Biochim Pol, 63(2):377-86 . details
  • Bochtler M., Piasecka A. Haloferax volcanii UbaA, catalytic engine for sampylation and sulfur transfer. 2016. FEBS J, 283(19):3563-3566 . details
  • Mierzejewska K, Bochtler M, Czapinska H. On the role of steric clashes in methylation control of restriction endonuclease activity. 2016. Nucleic Acids Res, 44(1) 485-95. details
  • 2015
  • Haniewicz P, Floris D, Farci D, Kirkpatrick J, Loi MC, Büchel C, Bochtler M, Piano D. Isolation of Plant Photosystem II Complexes by Fractional Solubilization. 2015. Front Plant Sci, 6 1100. details
  • Grabowska M, Jagielska E, Czapinska H, Bochtler M, Sabala I. High resolution structure of an M23 peptidase with a substrate analogue. 2015. Sci Rep, 5:14833 . details
  • Burmistrz M, Dudek B, Staniec D, Rodriguez Martinez JI, Bochtler M, Potempa J, Pyrc K. Functional Analysis of Porphyromonas gingivalis W83 CRISPR-Cas Systems. 2015. J Bacteriol, 197(16) 2631-41. details
  • 2014
  • Gallagher JM, Yamak A, Kirilenko P, Black S, Bochtler M, Lefebvre C, Nemer M, Latinkić BV. Carboxy terminus of GATA4 transcription factor is required for its cardiogenic activity and interaction with CDK4. 2014. Mech Dev, 134 31-41. details
  • Mierzejewska K, Siwek W, Czapinska H, Kaus-Drobek M, Radlinska M, Skowronek K, Bujnicki JM, Dadlez M, Bochtler M. Structural basis of the methylation specificity of R.DpnI. 2014. Nucleic Acids Res., 42(13) 8745-54. details
  • Wojciechowski M, Rafalski D, Kucharski R, Misztal K, Maleszka J, Bochtler M, Maleszka R. Insights into DNA hydroxymethylation in the honeybee from in-depth analyses of TET dioxygenase. 2014. Open Biol, 4(8) 140110. details
  • Kazrani AA, Kowalska M, Czapinska H, Bochtler M. Crystal structure of the 5hmC specific endonuclease PvuRts1I. 2014. Nucleic Acids Res., 42(9) 5929-36. details
  • Jaremko M, Jaremko L, Nowakowski M, Wojciechowski M, Szczepanowski RH, Panecka R, Zhukov I, Bochtler M, Ejchart A. NMR structural studies of the first catalytic half-domain of ubiquitin activating enzyme. 2014. J Struct Biol, 185(1) 69-78. details
  • 2013
  • Haniewicz P, De Sanctis D, Büchel C, Schröder WP, Loi MC, Kieselbach T, Bochtler M, Piano D. Isolation of monomeric photosystem II that retains the subunit PsbS. 2013. Photosynth Res, 118(3) 199-207. details
  • Wojciechowski M, Czapinska H, Bochtler M. CpG Underrepresentation and the Bacterial CpG Specific DNA Methyltransferase M.MpeI. 2013. Proc Natl Acad Sci U S A., 110(1) 105-10. details
  • 2012
  • Bochtler M. Structural basis of the TAL effector-DNA interaction. 2012. Biol Chem, 393(10) 1055-66. details
  • Sabala I, Jonsson IM, Tarkowski A, Bochtler M. Anti-staphylococcal activities of lysostaphin and LytM catalytic domain. 2012. BMC Microbiol, 12 97. details
  • 2011
  • Antonczak AK, Simova Z, Yonemoto IT, Bochtler M, Piasecka A, Czapinska H, Brancale A, Tippmann EM. Importance of single molecular determinants in the fidelity of expanded genetic codes. 2011. Proc Natl Acad Sci U S A., 108(4) 1320-1325. details
  • Braun S, Humphreys C, Fraser E, Brancale A, Bochtler M, Dale TC. Amyloid-associated nucleic acid hybridisation. 2011. PLoS One, 6(5) e19125. details
  • Firczuk M, Wojciechowski M, Czapinska H, Bochtler M. DNA intercalation without flipping in the specific ThaI-DNA complex. 2011. Nucleic Acids Res, 39 744-54. details
  • Sokolowska M, Czapinska H, Bochtler M. Hpy188I-DNA pre- and post-cleavage complexes--snapshots of the GIY-YIG nuclease mediated catalysis. 2011. Nucleic Acids Res, 39(4) 1554-64. details
  • 2010
  • Chojnowski G, Bochtler M. DIBER: protein, DNA or both?. 2010. Acta Crystallogr D Biol Crystallogr, 66 643-53. details
  • Gentsch M, Kaczmarczyk A, van Leeuwen K, de Boer M, Kaus-Drobek M, Dagher MC, Kaiser P, Arkwright PD, Gahr M, Rösen-Wolff A, Bochtler M, Secord E, Britto-Williams P, Saifi GM, Maddalena A, Dbaibo G, Bustamante J, Casanova JL, Roos D, Roesler J. Alu-repeat-induced deletions within the NCF2 gene causing p67-phox-deficient chronic granulomatous disease (CGD). 2010. Hum Mutat, 31 151-8. details
  • Chojnowski G, Breer K, Narczyk M, Wielgus-Kutrowska B, Czapinska H, Hashimoto M, Hikishima S, Yokomatsu T, Bochtler M, Girstun A, Staroń K, Bzowska A. 1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state. 2010. Biochem Biophys Res Commun, 391 703-8. details
  • Piano D, El Alaoui S, Korza HJ, Filipek R, Sabala I, Haniewicz P, Buechel C, De Sanctis D, Bochtler M. Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotiana tabacum. 2010. Photosynth Res, 39(4) 221-6. details
  • 2009
  • Sokolowska M, Czapinska H, Bochtler M. Crystal structure of the beta beta alpha-Me type II restriction endonuclease Hpy99I with target DNA. 2009. Nucleic Acids Res, 37 3799-810. details
  • 2008
  • Szczepanowski RH, Carpenter MA, Czapinska H, Zaremba M, Tamulaitis G, Siksnys V, Bhagwat AS, Bochtler M. Central base pair flipping and discrimination by PspGI. 2008. Nucleic Acids Res, 36 6109-17. details
  • Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V. How PspGI, catalytic domain of EcoRII and Ecl18kI acquire specificities for different DNA targets. 2008. Nucleic Acids Res, 36 6101-8. details
  • Breer K, Wielgus-Kutrowska B, Hashimoto M, Hikishima S, Yokomatsu T, Szczepanowski RH, Bochtler M, Girstun A, Starón K, Bzowska A. Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors. 2008. Nucleic Acids Symp Ser (Oxf), 663-4. details
  • Sukackaite R, Grazulis S, Bochtler M, Siksnys V.. The recognition domain of the BpuJI restriction endonuclease in complex with cognate DNA at 1.3-A resolution. 2008. J Mol Biol, 378(5) 1084-93. details
  • Lipka M, Filipek R, Bochtler M. Crystal structure and mechanism of the Staphylococcus cohnii virginiamycin B lyase (Vgb). 2008. Biochemistry, 47 4257-65. details
  • 2007
  • Bochtler M, Chojnowski G. The highest reflection intensity in a resolution shell. 2007. Acta Crystallogr A, 63 146-55. details
  • Chojnowski G, Bochtler M. The statistics of the highest E value. 2007. Acta Crystallogr A, 63 297-305. details
  • Firczuk M, Bochtler M. Folds and activities of peptidoglycan amidases. 2007. FEMS Microbiol Rev, 31 676-91. details
  • Firczuk M, Bochtler M. Mutational analysis of peptidoglycan amidase MepA. 2007. Biochemistry, 46 120-8. details
  • Kaus-Drobek M, Czapinska H, Sokołowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M. Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically. 2007. Nucleic Acids Res, 35 2035-46. details
  • Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Siksnys V, Bochtler M . Restriction endonucleases that resemble a component of the bacterial DNA repair machinery. 2007. Cell Mol Life Sci, 64 2351-7. details
  • Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M. Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.. 2007. J Mol Biol, 369 722-34. details
  • Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V. Nucleotide flipping by restriction enzymes analyzed by 2-aminopurine steady-state fluorescence. 2007. Nucleic Acids Res, 35 4792-9. details
  • 2006
  • Bochtler M, Szczepanowski RH, Tamulaitis G, Grazulis S, Czapinska H, Manakova E, Siksnys V. Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease. 2006. EMBO J, 25 2219-29. details
  • 2005
  • Azim MK, Goehring W, Song HK, Ramachandran R, Bochtler M, Goettig P. Characterization of the HslU chaperone affinity for HslV protease. 2005. Protein Sci, 14 1357-62. details
  • Dandanell G,Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M. Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene. 2005. J Mol Biol, 348 113-25. details
  • Filipek R, Potempa J, Bochtler M. A comparison of staphostatin B with standard mechanism serine protease inhibitors. 2005. J Biol Chem, 280 14669-74. details
  • Firczuk M, Mucha A, Bochtler M. Crystal structures of active LytM. 2005. J Mol Biol, 354 578-90. details
  • Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V. Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.. 2005. Proc Natl Acad Sci U S A, 102 15797-802. details
  • Groll M, Bochtler M, Brandstetter H, Clausen T, Huber R. Molecular machines for protein degradation.. 2005. Chembiochem, 6 222-56. details
  • Korza HJ, Bochtler M. Pseudomonas aeruginosa LD-carboxypeptidase, a serine peptidase with a Ser-His-Glu triad and a nucleophilic elbow.. 2005. J Biol Chem, 280 40802-12. details
  • Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M. Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan. 2005. J Biol Chem, 280 27792-9. details
  • Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M. Substrate access to the active sites in aminopeptidase T, a representative of a new metallopeptidase clan.. 2005. J Mol Biol, 354 403-12. details
  • Szczepanowski RH, Filipek R, Bochtler M. Crystal structure of a fragment of mouse ubiquitin-activating enzyme.. 2005. J Biol Chem, 280 22006-11. details
  • Potempa J, Golonka E, Filipek R, Shaw LN. Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases. 2005. Mol Microbiol, 57 605-10. details
  • Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M. Peptydoglycan amidase MepA is a LAS metallopeptidase. 2005. J Biol Chem, 279 43982-89. details
  • 2004
  • Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M. Peptidoglycan amidase MepA is a LAS metallopeptidase.. 2004. J Biol Chem, 279 43982-9. details
  • Bochtler M, Odintsov SG, Marcyjaniak M, Sabala I. Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases.. 2004. Protein Sci, 13 854-61. details
  • Filipek R, Szczepanowski R, Sabat A, Potempa J, Bochtler M. Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition. 2004. Biochemistry, 43 14306-15. details
  • Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M. Peptidoglycan amidase MepA is a LAS metallopeptidase. 2004. J Biol Chem, 279 43982-9. details
  • Odintsov SG, Sabala I, Marcyjaniak M, Bochtler M. Latent LytM at 1.3A resolution. 2004. J Mol Biol, 335 775-85. details
  • Golonka E, Filipek R, Sabat A, Sinczak A, Potempa J. Genetic characterization of staphopain genes in Staphylococcus aureus. 2004. Biol Chem, 385 1059-67. details
  • 2003
  • Dubin G, Krajewski M, Popowicz G, Stec-Niemczyk J, Bochtler M, Potempa J, Dubin A, Holak TA. A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus.. 2003. Biochemistry, 42 13449-56. details
  • Filipek R, Rzychon M, Oleksy A, Gruca M, Dubin A, Potempa J, Bochtler M. The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease.. 2003. J Biol Chem, 278 40959-66. details
  • Rzychon M, Filipek R, Sabat A, Kosowska K, Dubin A, Potempa J, Bochtler M. Staphostatins resemble lipocalins, not cystatins in fold. 2003. Protein Sci, 12 2252-6. details
  • 2002
  • Ramachandran R, Hartmann C, Song HK, Huber R, Bochtler M. Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY). 2002. Proc Natl Acad Sci U S A, 99 7396-401. details
  • 2001
  • Palczewska M, Groves P, Ambrus A, Kaleta A, Kövér KE, Batta G, Kuźnicki J. Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93). 2001. Eur J Biochem, 268 6229-37. details