Laboratory of Mitochondrial Biogenesis: Chacinska Laboratory

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Agnieszka Chacińska, PhD, Professor

achacinska@iimcb.gov.pl
+48 22 5970779

 

 

 

 

Education and Degrees:
2014  Professor of Biological Sciences, nomination by the President of the Republic of Poland
2008  DSc Habil, Institute of Biochemistry and Biophysics, Warsaw, Poland
2000  PhD in Biochemistry, Institute of Biochemistry and Biophysics, Warsaw
1993  Master Degree in Molecular Biology, University of Warsaw
1988-1993  Biology, University of Warsaw, Poland


Awards:
2016 Award from the Premiere for the scientific achievements
2016 Elected EMBO member
2016 Laureate together with Peter Rehling of Nicolaus Copernicus Polish-German Research Award given jointly by the Foundation for Polish Science (FNP)
         and the German Research Foundation (DFG)
2015 Award from the Minister of Science and Higher Education for scientific achievements that led to the title of Professor
2015 Award from the President of Polish Academy of Science for scientific achievement
2010  EMBO Installation Grant
2009  Welcome Programme, Foundation for Polish Science
2008  Eugen-Graetz Prize for Research, University of Freiburg, Germany
2001-2003  Long-term FEBS fellowship
2001  Award for PhD thesis, Institute of Biochemistry and Biophysics, Warsaw
1997  Grant for young scientists, Polish State Committee for Scientific Research
1996  Short-term FEBS fellowship


Research experience and appointments:
2015 – present Deputy Director of Development at the International Institute of Molecular and Cell Biology in Warsaw, Poland
2009 - present  Professor and Leader of Laboratory of Mitochondrial Biogenesis, International Institute of Molecular and Cell Biology, Warsaw, Poland
2008-2009  Associate Member of Excellence Cluster BIOSS, Centre for Biological Signalling Studies, University of Freiburg, Germany
2007-2009  Member of the Board, Collaborative Research Centre (SFB 746)
2007-2010  Project Leader in Collaborative Research Centre (SFB 746), Germany
2004-2009  Group Leader (German equivalent of Assistant Professor), Institute for  Biochemistry and Molecular Biology, University of Freiburg, Germany
2001-2004  Postdoctoral Fellow, Laboratory of Prof. Nikolaus Pfanner, University of Freiburg, Germany
1999  Visiting Scientist, Laboratory of Prof. Sabine Rospert, Max Planck Research Unit, Halle, Germany
1997  Visiting Scientist, Laboratory of Prof. Gottfried Schatz, Biozentrum, University of Basel, Switzerland
1994-2000  Doctoral research with Prof. Magdalena Boguta, Institute of Biochemistry and Biophysics, Warsaw, Poland

 
Agnieszka Chacinska's profile in Science:
http://sciencecareers.sciencemag.org/career_magazine/previous_issues/articles/2010_03_19/caredit.a1000027

 

For recent publications see Research Focus and Publications

  
Pubmed search for Chacinska A

Selected publications from my group in Freiburg:

Chacinska, A., Koehler, C.M., Milenkovic, D., Lithgow, T., Pfanner, N. (2009) Importing mitochondrial proteins: machineries and mechanisms. Cell 138, 628-644. 

Milenkovic, D., Ramming, T., Müller, J.M., Wenz, L.S., Gebert, N., Schulze-Specking, A., Stojanovski, D., Rospert, S., Chacinska, A.  (2009) Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria. Mol. Biol. Cell 20, 2530-2539.

Stojanovski, D., Milenkovic, D., Müller, J.M., Gabriel, K., Schulze-Specking, A., Baker, M.J., Ryan, M.T., Guiard, B., Pfanner, N., Chacinska, A. (2008) Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase. J. Cell Biol. 183, 195-202.

Müller, J.M., Milenkovic, D., Guiard, B., Pfanner, N., Chacinska A. (2008) Precursor oxidation by Mia40 and Erv1 promotes vectorial transport of proteins into the mitochondrial intermembrane space. Mol. Biol Cell. 19, 226-236.

Stojanovski, D., Müller, JM, Milenkovic, D., Guiard, B., Pfanner, N., Chacinska, A. (2008) The MIA system for protein import into the mitochondrial intermembrane space. Biochim. Biophys. Acta, 1783, 610-617.

Milenkovic, D., Gabriel, K., Guiard, B., Schulze-Specking, A., Pfanner, N., Chacinska, A. (2007) Biogenesis of the essential Tim9-Tim10 chaperone complex of mitochondria: site-specific recognition of cysteine residues by the intermemembrane space receptor Mia40. J. Biol. Chem. 282, 22472-22480.

Wiedemann, N., Pfanner, N., and Chacinska, A. (2006). Chaperoning through the mitochondrial intermembrane space. Mol. Cell 21, 145-148.

Rissler, M., Wiedemann, N., Pfannschmidt, S., Gabriel, K., Guiard, B., Pfanner, N., and Chacinska, A. (2005). The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins. J. Mol. Biol. 353, 485-492.

Selected publications arising from my post-doctoral research:

Chacinska, A.*, Lind, M.*, Frazier, A.E., Dudek, J., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2005). Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829 (*equal contribution).

van der Laan, M.*, Chacinska, A.*, Lind, M., Perschil, I., Sickmann, A., Meyer, H.E., Guiard, B., Meisinger, C., Pfanner, N., and Rehling, P. (2005). Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol. Cell. Biol. 25, 7449-7458 (*equal contribution).

Chacinska, A., Pfannschmidt, S., Wiedemann, N., Kozjak, V., Sanjuán Szklarz, L.K., Schulze-Specking, A., Truscott, K.N., Guiard, B., Meisinger, C., and Pfanner, N. (2004). Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J. 23, 3735-3746.

Chacinska, A., Rehling, P., Guiard, B., Frazier, A.E., Schulze-Specking, A., Pfanner, N., Voos, W., and Meisinger, C. (2003). Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 22, 5370-5381.

Geissler, A.*, Chacinska, A.*, Truscott, K.N., Wiedemann, N., Brandner, K., Sickmann, A., Meyer, H.E., Meisinger, C., Pfanner, N., and Rehling, P. (2002). The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111, 507-518 (*equal contribution).

Other important contributions:

Meinecke, M., Wagner, R., Kovermann, P., Guiard, B., Mick, D.U., Hutu, D.P., Voos, W., Truscott, K.N., Chacinska, A., Pfanner, N., and Rehling, P. (2006). Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 312, 1523-1526.

Meisinger, C., Rissler, M., Chacinska, A., Sanjuán Szklarz, L.K., Milenkovic, D., Kozjak, V., Schönfisch, B., Lohaus, C., Meyer, H.E., Yaffe, M.P., Guiard, B., Wiedemann, N., and Pfanner, N. (2004). The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane. Dev. Cell 7, 61-71.

Sickmann, A., Reinders, J., Wagner, Y., Joppich, C. Zahedi, R., Meyer, H.E., Schönfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., and Meisinger, C. (2003). The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. USA 100, 13207-13212.

Wiedemann, N., Kozjak, V., Chacinska, A., Schönfisch, B., Rospert, S., Ryan, M.T., Pfanner, N., and Meisinger, C. (2003). Machinery for protein sorting and assembly in the mitochondrial outer membrane. Nature 424, 565-571.

The most important paper from my PhD studies:

Chacinska A., Boguta M., Krzewska J. and Rospert S. (2000) Prion-dependent switching between respiratory competence and deficiency in the yeast nam9-1 mutant. Mol. Cell. Biol. 20, 7220-9.