The International Institute of Molecular and Cell Biology

Speakers:

Jacek Kuźnicki HEALTH‑PROT project at the IIMCB and its international and domestic extension
Ewa Łojkowska Intercollegiate Faculty of Biotechnology UG and MUG – a unique example of the interaction between two universities
MacieJ Żylicz MDM2’s dilemma: to be an oncogene or tumour suppressor
Janusz M. Bujnicki From bioinformatics to protein engineering
Matthias Bochtler A new CG methyltransferase
Marta Miączyńska Multiple roles of endocytic proteins in the regulation of signal transduction
Jacek Jaworski Neuronal dendritic arbor, a playground for understanding non‑canonical functions of mTOR
Michał Witt Cilia biology, a key to multiple pathologies
Agnieszka Chacińska Redox‑driven protein import into mitochondria – nothing by chance
Marcin Nowotny Structural studies of a monomeric reverse transcriptase
Roman Szczepanowski Presentation of principal scientific equipment available at IIMCB
Jacek Kuźnicki Calcium and beta‑catenin signaling in the brain

[                                                                                                                                                                               

 International Institute of Molecular and Cell Biology in Warsaw

invites to the seminar and equipment presentation:

Stephen Blanke, PhD

 NanoTemper Technologies GmbH, Munich, Germany

Some like it hot  -  Biomolecule Analytics using Microscale Thermophoresis (MST)

The presentation gives an overview of Microscale Thermophoresis (MST), a new technology for the measurement of biomolecule interactions. The term Microscale Thermophoresis refers to the directed movement of molecules in optically generated microscopic temperature gradients. This thermophoretic movement is determined by the entropy of the hydration shell around the molecules. Almost all interactions between molecules and virtually any biochemical process are linked to a change in size, charge and/or conformation of molecules which alter this hydration shell and therefore can be detected and quantified by MST.

Microscale Thermophoresis allows quantification of binding affinities of proteins, nucleic acids and small molecules as well as measurement of enzymatic activities. In addition also functional studies of small molecule inhibitors are possible. The steep microscopic temperature gradient is generated by an IR-Laser, which is strongly absorbed by water. The readout method of the interaction analysis is based on fluorescence: fluorescently labeled proteins/peptides/nucleic acids can be used as well as intrinsic tryptophane fluorescence or proteins expressed with GFP/YFP/RFP.

The presentation will cover: 1) Technical details and benefits of the Microscale Thermophoresis technology platform and 2) Examples of interaction measurements ranging from protein – ribosome, protein – protein, small molecule – receptor down to protein – ion binding studies to experiments where the interactions between receptors incorporated in vesicles and soluble proteins are analyzed.

PhD Report Session #2
The International Institute of Molecular and Cell Biology in Warsaw (IIMCB)
January 12th (Thursday) 2012, the lecture hall 012,
ground floor

TOP500 Innovators “Science-Management-Commercialization” program is organized by Ministry of Science and Higher Education.

In 2011 we have visited Stanford University and now we would like to share with you our experiences, impressions and answer your questions.

discussion will be held *in Polish on January 17th (Tuesday) 2012, at 11:00 a.m.

at the International Institute of Molecular and Cell Biology in Warsaw

ul. Ks. Trojdena 4, the lecture hall 0-12, ground floor