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International Institute of Molecular and Cell Biology
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About IIMCB Authorities
Jacek Kuznicki

Jacek Kuznicki, PhD, Professor

b.1952; MSc in biochemistry, Warsaw University, 1976; PhD in biochemistry, Nencki Institute, 1980; post-doctoral 1981-1984, NIH, Bethesda, MD, USA; 1986-2002, head of the laboratory, Nencki Institute; habilitation 1987; Scientific Director at the Nencki Institute 1990-1992; Secretary of the Polish Biochemical Society, 1989-1992; Full Professor 1993; Co-Editor of “Advances in Biochemistry” (in Polish), 1989-1992; Visiting Professor 1992-1995, NIH, Bethesda, MD, USA; Member of Advisory Boards of Biochem J and Acta Biochem Pol, Acting Director at the International Institute of Molecular and Cell Biology in Warsaw 1998-2001; Director of Center of Excellence for Studies on Mechanisms of Neurodegeneration located at the Nencki Institute, 1999-2000; Member of Polish Academy of Sciences, 2004; Member of the 7th FP European Commission – Health Research Advisory Group, since 2006, Member of the European Calcium Society Board, since 2008; Member of Prime Minister Award Committee, 2007 – 2009; Member of the Polish Alzheimer’s Society, since 2009, Member of the Society for Neuroscience since 2010; Director at the International Institute of Molecular and Cell Biology in Warsaw, Head of the Laboratory of Neurodegeneration, 2002-present.

Current Research:

  • Alzheimer’s disease and other neurodegenerative diseases
  • Ageing and longevity
  • Protein structure-function analysis
  • Calcium regulation and homeostasis
  • Calcium-binding proteins
  • Cell specific gene expression

Chief achievements:

  • Description of a new role of beta-catenin in neurons as activator of transcription Cacna1g gene (2010),
  • Identification of STIM-ORAI in neurons and involvement in Capacitative Calcium Entry (2008-2009),
  • Characterization of calmyrin and its protein partners (2005-2007),
  • Characterization of Sgt1 protein, homolog of CacyBP/SIP (2003-2009),
  • Identification of novel FAD and FTD mutations in presenilins (2003-2006),
  • Organization of the Polish Centenarians Program (1998-2002),
  • Description of mechanisms involved in expression of calcyclin gene (1996-2006),
  • Discovery and characterization of a novel brain protein involved in ubiquitination (CacyBP/SIP) (1993-2009),
  • Presenting evidence for calcium dependent conformational changes of calretinin (1993-1996),
  • Discovery, purification, and characterization of calcyclin (S100A6) (1985-1992),
  • Presenting evidence for supramolecular regulation of actin-activated myosin II ATPase from Acanthamoeba castellani (1982-1984),
  • Characterization of calcium-dependent conformational changes of calmodulin, its distribution, and discovery of calmodulin in Protozoa (1976-1981).


Supervisor of 9 Ph.D. theses defended in 1990, 1992, 1995, 2001, 2002(2x), 2003, 2008, 2009.

Author of (113) articles cited by Medline and published in J Biol Chem (9 papers), FEBS Lett (7), Biochim Biophys Acta (10), J Neurochem (3), Biochem J (3), Biochemistry (3), Acta Biochimica Polonica (4), Prot Exp Purif (2), Eur J Biochem (2), Prot Sci (2), Neurochem Int (2), Exp Neurol (2), Neurosci Lett (2), Int J Biochem (4), Cancer Res, Exp Cell Res, NeuroReport, Eur J Cancer, Mol Brain Res, Neurogenetics, Mol Psychiatry, J Histochem Cytochem, Adv Enzyme Regul., Adv Exp Med Biol., Ann N Y Acad. Sci., Biochem Mol Biol Int., Brain Res Mol Brain Res., Acta Neurobiol Exp (Wars), J Neurosci. and other.

Selected publications since 1995:

Wisniewska M, Misztal K, Michowski W, Szczot M, Purta E, Lesniak W, Klejman M, D¹browski M, Filipkowski R, Nagalski A, Mozrzymas J, Kuznicki J. LEF1/beta-catenin complex regulates transcription of the Cav3.1 calcium channel gene (Cacna1g) in thalamic neurons of the adult brain. Journal of Neuroscience. 2010, accepted

Blazejczyk M, Sobczak A, Debowska K, Wisniewska MB, Kirilenko A, Pikula S, Jaworski J, Kuznicki J, Wojda U. Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2)in brain indicates its function in NMDA receptor mediated Ca2+ signaling. Arch Biochem Biophys. 2009 Jul 1;487(1):66-78. Epub 2009 May 9;

Puzianowska-Kuznicka M, Kuznicki J. The ER and Ageing II: Calcium homeostasis. Ageing Res Rev. 2009 Jul;8(3):160-72. Epub 2009 May 7;

Bojarski L, Pomorski P, Szybinska A, Drab M, Skibinska-Kijek A, Gruszczynska-Biegala J, Kuznicki J. Presenilin-dependent expression of STIM proteins and dysregulation of capacitative Ca(2+) entry in familial Alzheimer's disease. Biochim Biophys Acta. Mol Cell Res. 2008 Dec 6;

Klejman ME, Gruszczynska-Biegala J, Skibinska-Kijek A, Wisniewska MB, Misztal K, Blazejczyk M, Bojarski L, Kuznicki J. Expression of STIM1 in brain and puncta-like co-localization of STIM1 and ORAI1 upon depletion of Ca(2+) store in neurons. Neurochem Int. 2009 Jan;54(1):49-55. Epub 2008 Nov 1;

Erickson RP, Larson-Thomé K, Weberg L, Szybinska A, Mossakowska M, Styczynska M, Barcikowska M, Kuznicki J. Variation in NPC1, the gene encoding Niemann-Pick C1, a protein involved in intracellular cholesterol transport,is associated with Alzheimer disease and/or aging in the Polish population. Neurosci Lett. 2008 Dec 12;447(2-3):153-7. Epub 2008 Sep 24;

Wojda U, Salinska E, Kuznicki J. Calcium ions in neuronal degeneration. IUBMB Life. 2008 Sep;60(9):575-90. Review;

Sbroggiò M, Ferretti R, Percivalle E, Gutkowska M, Zylicz A, Michowski W, Kuznicki J, Accornero F, Pacchioni B, Lanfranchi G, Hamm J, Turco E, Silengo L, Tarone G, Brancaccio M. The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1. FEBS Lett. 2008 Jun 11;582(13):1788-94. Epub 2008 May 12;

Zabka M, Lesniak W, Prus W, Kuznicki J, Filipek A. Sgt1 has co-chaperone properties and is up-regulated by heat shock. Biochem Biophys Res Commun. 2008 May 23;370(1):179-83. Epub 2008 Mar 19;

Filipek A, Michowski W, Kuznicki J. Involvement of S100A6 (calcyclin) and its binding partners in intracellular signaling pathways. Adv Enzyme Regul. 2008;48:225-39. Epub 2007 Nov 19. Review;

Mossakowska M, Barcikowska M, Broczek K, Grodzicki T, Klich-Raczka A, Kupisz-Urbanska M, Podsiadly-Moczydlowska T, Sikora E, Szybinska A, Wieczorowska-Tobis K, Zyczkowska J, Kuznicki J. Polish Centenarians Programme. Multidisciplinary studies of successful ageing: aims, methods, and preliminary results. Exp Gerontol. 2008 Mar;43(3):238-44. Epub 2007 Nov 6;

Bojarski L, Herms J, Kuznicki J. Calcium dysregulation in Alzheimer's disease. Neurochem Int. 2008 Mar-Apr;52(4-5):621-33. Epub 2007 Oct 5. Review.

Lanni C, Racchi M, Mazzini G, Ranzenigo A, Polotti R, Sinforiani E, Olivari L, Barcikowska M, Styczynska M, Kuznicki J, Szybinska A, Govoni S, Memo M, Uberti D. Conformationally altered p53: a novel Alzheimer's disease marker? Mol Psychiatry. 2008 Jun;13(6):641-7. Epub 2007 Aug 7;

Bojarski L, Lewandowicz A, Blazejczyk M, Sobczak A, Kuznicki J, Wojda U. Biochemical properties of endogenous presenilin 1 and presenilin 2 in cultured human B-lymphocytes. Clin Chem Lab Med, 2007; 45:1273-6;

Lesniak W, Slomnicki LP, Kuznicki J. Epigenetic control of the S100A6 (calcyclin) gene expression. J Invest Dermatol. 2007 Oct;127(10):2307-14. Epub 2007 May 10;

Wollmer MA, Sleegers K, Ingelsson M, Zekanowski C, Brouwers N, Maruszak A, Brunner F, Huynh KD, Kilander L, Brundin RM, Hedlund M, Giedraitis V, Glaser A, Engelborghs S, De Deyn PP, Kapaki E, Tsolaki M, Daniilidou M, Molyva D, Paraskevas GP, Thal DR, Barcikowska M, Kuznicki J, Lannfelt L, Van Broeckhoven C, Nitsch RM, Hock C, Papassotiropoulos A. Association study of cholesterol-related genes in Alzheimer's disease. Neurogenetics. 2007 Aug;8(3):179-88. Epub 2007 Mar 27;

Schneider G, Nieznanski K, Kilanczyk E, Bieganowski P, Kuznicki J, Filipek A. CacyBP/SIP interacts with tubulin in neuroblastoma NB2a cells and induces formation of globular tubulin assemblies. Biochim Biophys Acta. 2007 Nov;1773(11):1628-36;

Spiechowicz M, Zylicz A, Bieganowski P, Kuznicki J, Filipek A. Hsp70 is a new target of Sgt1--an interaction modulated by S100A6. Biochem Biophys Res Commun.2007Jun15;357(4):1148-53;

Spiechowicz M, Bernstein HG, Dobrowolny H, Lesniak W, Mawrin C, Bogerts B, Kuznicki J, Filipek A, Density of Sgt1-immunopositive neurons is decreased in the cerebral cortex of Alzheimer's disease brain: Neurochem Int. 2006 Oct;49(5):487-93;

Golan M.P, Krzysko K.A, Lipczynska-Lojkowska W, Filipek S, Kowalska A, Rossa G, Peplonska B, Styczynska M, Maruszak A, Religa D, Wender M, Kulczycki J, Barcikowska M, Kuznicki J. Two novel presenilin 1 gene mutations connected with frontotemporal dementia-like clinical phenotype: genetic and bioinformatic assessment. Exp Neurol. 2006 Jul;200(1):82-88;

Blazejczyk M, Wojda U, Sobczak A, Spilker C, Bernstein H-G, Gundelfinger ED, Kreutz MR, Kuznicki J, (2006) Ca2+-independent binding and cellular expression profiles question a significant role of calmyrin in transduction of Ca2+-signals to Alzheimer's disease-related presenilin 2 in forebrain. Biochim Biophys Acta - Molecular Basis of Disease 1762(1):66-72;

Lesniak W, Kuznicki J. Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter. J Cell Biochem. 2006 Apr 1;97(5):1017-24;

Sobczak A, Blazejczyk M, Piszczek G, Zhao G, Kuznicki J, Wojda U (2005) Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form. Acta Biochim Pol. 52(2):469-76;

Bernstein HG, Blazejczyk M, Rudka T, Gundelfinger ED, Dobrowolny H, Bogerts B, Kreutz MR, Kuznicki J, Wojda U (2005) The Alzheimer disease-related calcium-binding protein calmyrin is prominently expressed in human forebrain with an altered distribution in Alzheimer's as compared to normal aging brains. Neuropathol Appl Neurobiol 31(3):314-24;

Bhattacharya S, Lee YT, Michowski W, Jastrzebska B, Filipek A, Kuznicki J, Chazin WJ (2005) The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies. Biochemistry. 44(27):9462-71;

Lesniak W, Szczepanska A, Kuznicki J (2005) Calcyclin (S100A6) expression is stimulated by agents evoking oxidative stress via the antioxidant response element. Biochim Biophys Acta. 2005 May 15;1744(1):29-37;

Palczewska M, Batta G, Groves P, Linse S, Kuznicki J (2005) Characterization of calretinin I-II as an EF-hand, Ca2+, H+-sensing domain. Protein Sci. 2005 Jul;14(7):1879-87;

Puzianowska-Kuznicka M, Kuznicki J (2005) Genetic alterations in accelerated ageing syndromes. Do they play a role in natural ageing? Int J Biochem Cell Biol. 37(5):947-60;

Zekanowski C, Religa D, Safranow K, Maruszak A, Dziedziejko V, Styczynska M, Gacia M, Golan M, Peplonska B, Chlubek D, Kuznicki J, Barcikowska M (2005) The -22c/t polymorphism in presenilin 1 gene is not connected with late-onset and early-onset familial Alzheimer's disease in Poland. J Neural Transm. 112(6):839-45;

Witt M, Ziemka A, Kuznicki J (2005) International Institute of Molecular and Cell Biology in Warsaw (in Polish). Postepy Biochemii 353-6


Billing-Marczak K, Zieminska E, Lesniak W, Lazarewicz JW, Kuznicki J (2004) Calretinin gene promoter activity is differently regulated in neurons and cancer cells. Role of AP2-like cis element and zinc ions. Biochim Biophys Acta 16; 1678(1): 14-21

Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ (2004) Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain. J Biol Chem 279: 16511-16517

Zekanowski C, Peplonska B, Styczynska M, Religa D, Pfeffer A, Czyzewski K, Gabryelewicz T, Szybinska A, Kijanowska-Haladyna B, Kotapka-Minc S, Luczywek E, Barczak A, Wasiak B, Chodakowska-Zebrowska M, Przekop I, Kuznicki J, Barcikowska M (2004) The E318G substitution in PSEN1 gene is not connected with Alzheimer's disease in a large Polish cohort. Neurosci. Lett 357(3): 167-170

Zekanowski C, Religa D, Graff C, Filipek S, Kuznicki J (2004) Genetic aspects of Alzheimer’s disease. Acta Neurobiol Exp 64: 19-31

M. Nowotny, M. Spiechowicz, B. Jastrzebska, A. Filipek, K Kitagawa, J. Kuznicki (2003) Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins. J. Biol. Chem. 278(29): 26923-26928

B. Peplonska, C. Zekanowski, D. Religa, K. Czyzewski, M. Styczynska, A. Pfeffer, T. Gabryelewicz, M. Golebiowski, E. Luczywek, B. Wasiak, A. Barczak, M. Chodakowska, M. Barcikowska, J. Kuznicki (2003) Strong association between Saitohin gene polymorphism and tau haplotype in the Polish population. Neurosci. Lett. 348(3): 163-166

C. Zekanowski, M. Styczynska, B. Peplonska, T. Gabryelewicz, D. Religa, J. Ilkowski, B. Kijanowska-Haladyna, S. Kotapka-Minc, S. Mikkelsen, A. Pfeffer, A. Barczak, E. Luczywek, B. Wasiak, M. Chodakowska-Zebrowska, K. Gustaw, J. Laczkowski, T. Sobow, J. Kuznicki, M. Barcikowska (2003) Mutations in presenilin 1, presenilin 2 and amyloid precursor protein genes in patients with familial Alzheimer disease in Poland. Exp. Neurol. 184: 991-996

C. Zekanowski , B. Peplonska, M. Styczynska, K. Gustaw, J. Kuznicki, M. Barcikowska (2003) Mutation screening of the MAPT and the STH genes in Polish patients with clinically diagnosed frontotemporal dementia (FTD). Dement. Geriatr. Cogn. Disord. 16(3): 126-131


M. Palczewska, P. Groves, G. Batta, B. Heise, J. Kuznicki (2003) Calretinin and calbindin D28k have different domain organizations. Prot. Sci. 12: 180-184

K. Billing-Marczak, L. Buzanska, L. Winsky, M. Nowotny, T. Rudka, K. Isaacs, M.F. Belin, J. Kuznicki (2002) AP2-Like element is required for calretinin gene promoter activity in cells of neuronal phenotype differentiated from multipotent human cell line DEV. Biochim. Biophys. Acta 1577: 412-420

A. Filipek,B. Jastrzebska, M. Nowotny, J. Kuznicki (2002) CacyBP/SIP, a Calcyclin and Siah-1-interacting Protein, Binds EF-hand Proteins of the S100 Family. J. Biol. Chem. 277: 28848-52

A. Filipek, B. Jastrzebska, M. Nowotny, K. Kwiatkowska, M. Hetman, L. Surmacz, E. Wyroba and J. Kuznicki (2002) Ca2+-dependent translocation of the calcyclin-binding protein in Neurons and neuroblastoma NB-2a cells. J. Biol. Chem., 277: 21103-9

M. Palczewska, P. Groves, A. Ambrus, A. Kaleta, K. E. Kövér, G. Batta, J. Kuznicki (2001) Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100); comparison to homologous calbindin D28k domain I-II (residues 1-93). Eur. J. Biochem., 268: 6229-6237

M. Nowotny, S. Bhattacharya, A. Filipek, A. M. Krezel, W. Chazin, J. Kuznicki (2000) Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein. J. Biol. Chem. 275: 31178-31182

B. Jastrzebska, A. Filipek, D. Nowicka, L. Kaczmarek, J. Kuznicki. (2000) Calcyclin (S100A6) binding protein (CacyBP) is highly expressed in brain neurons. J. Histochem. Cytochem. 48: 1195-1202

W. Lesniak, A. Jezierska, J. Kuznicki (2000) Upstream stimulatory factor is involved in the regulation of the human calcyclin (S100A6) gene. Biochim. Biophys. Acta 1517: 73-81

M. Palczewska, P. Groves, J. Kuznicki (1999) Use of Pichia pastoris for expression, purification and characterization of rat calretinin “EF-hand” domains. Prot. Exp. Purif. 17: 465-464

K. Billing-Marczak, M. Przybyszewska, J. Kuznicki (1999) Measurements of [Ca2+] using fura-2 in glioma C6 cells expressing calretinin with GFP as a marker of transfection: no Ca2+-buffering provided by calretinin. Biochem. Biophys. Acta 1449: 169-177

A. Filipek, J. Kuznicki (1998) Molecular cloning and expression of a mouse brain cDNA encoding a novel protein target of calcyclin. J. Neurochem. 70: 1793-1798

P. Groves, B.E. Finn, J. Kuznicki, S. Forsen (1998) A model for target protein binding to calcium-activated S100 dimers. FEBS Lett. 421: 175-179

K.I. Strauss, J. Kuznicki, L. Winsky, M. Hammer, D.M. Jacobowitz (1996) The mouse calretinin gene promoter region: structural and functional components. Mol. Brain. Res. 49: 175-187

J. Kuznicki, K. R. Isaacs, D.M. Jacobowitz (1996) The expression of calretinin in transfected PC12 cells provides no protection against Ca2+-overload or trophic factors deprivation. Biochem. Biophys. Acta 1313: 194-200

J. Kuznicki (1996) Calcyclin. In: Guidebook to the Calcium-binding Proteins, (ed. MR Celio). A Sambrook & Tooze Publication at Oxford University Press, 143-145

L. Winsky and J. Kuznicki (1996) Antibody recognition of calcium-binding proteins depends on their calcium-binding status. J. Neurochem. 66: 764-771

J. Kuznicki, K.J. Strauss and D.J. Jacobowitz (1995) Conformational changes and calcium binding by calretinin and its recombinant fragments containing different sets of EF-hand motifs. Biochemistry 34: 15389-15394

J. Kuznicki, T.-C. L. Wang, B.M. Martin, L. Winsky, D.M. Jacobowitz (1995) Localization of Ca2+- dependent conformational changes of calretinin by limited tryptic proteolysis. Biochem. J. 308: 607-612

G.P. Tonini, G. Fabretti, J. Kuznicki, L. Massimo, P. Scaruffi, M. Brisigotti, (1995) Gene expression and protein localization of calcyclin, a calcium-binding protein of the S-100 family in fresh neuroblastomas. Eur. J. Cancer 31A: 499-504

L. Winsky and J. Kuznicki (1995) Distribution of calretinin, calbindin D28k, and parvalbumin in subcellular fractions of rat cerebellum: effects of calcium. J. Neurochem. 65: 381-388.
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