All publications


Ravichandran M, Rafalski D, Davies CI, Ortega-Recalde O, Nan X, Glanfield CR, Kotter A, Misztal K, Wang AH, Wojciechowski M, Rażew M, Mayyas IM, Kardailsky O, Schwartz U, Zembrzycki K, Morison IM, Helm M, Weichenhan D, Jurkowska RZ, Krueger F, Plass C, Zacharias M, Bochtler M, Hore TA, Jurkowski TP.

Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function.

Sci Adv.

Niescierowicz K#, Pryszcz L#, Navarrete C#, Tralle E#, Sulej A#, Abu Nahia K, Kasprzyk ME, Misztal K, Pateria A, Pakuła A, Bochtler M, Winata C.

# Contributed equally

Adar-mediated A-to-I editing is required for embryonic patterning and innate immune response regulation in zebrafish.

Nat Commun.

Czapińska H, Bochtler M.

The Nε-rule for serine, but not cysteine catalytic triads.

Angew Chem Int Ed Engl.


Bochtler M.

Distinction between self and non-self in restriction modification: The mysterious case of type IIL enzymes.


Czapinska H, Winiewska-Szajewska M, Szymaniec-Rutkowska A, Piasecka A, Bochtler M, Poznański J.

Halogen Atoms in the Protein-Ligand System. Structural and Thermodynamic Studies of the Binding of Bromobenzotriazoles by the Catalytic Subunit of Human Protein Kinase CK2.

J Phys Chem B.

Pastor M, Czapinska H, Helbrecht I, Krakowska K, Lutz T, Xu S, Bochtler M.

Crystal structures of the EVE-HNH endonuclease VcaM4I in the presence and absence of DNA.

Nucleic Acids Res.

Bochtler M, Fernandes H.

DNA adenine methylation in eukaryotes: Enzymatic mark or a form of DNA damage?



Xu G-L, Bochtler M.

Reversal of nucleobase methylation by dioxygenases.

Nat Chem Biol

Fricke T, Smalakyte D, Lapinski M, Pateria A, Weige C, Pastor M, Kolano A, Winata C, Siksnys V, Tamulaitis G, Bochtler M.

Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish.


Lutz T, Czapinska H, Fomenkov A, Potapov V, Heiter DF, Cao B, Dedon P, Bochtler M, Xu S.

Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases.

Front. Microbiol.

Kisiala M, Kowalska M, Pastor M, Korza HJ, Czapinska H, Bochtler M.

Restriction endonucleases that cleave RNA/DNAheteroduplexes bind dsDNA in A-like conformation.

Nucleic Acids Res.

Skowronek KJ, Bochtler M.

In Vitro Directed Evolution of a Restriction Endonuclease With More Stringent Specificity.

J Vis Exp

Bochtler M.

Arrhenius-law-governed homo- and heteroduplex dissociation.

Phys Rev E


Slyvka A, Zagorskaitė E, Czapinska H, Sasnauskas G, Bochtler M.

Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping.

Nucleic Acids Res.

Lutz T, Flodman K, Copelas A, Czapinska H, Mabuchi M, Fomenkov A, He X, Bochtler M, Xu S.

A protein architecture guided screen for modification dependent restriction endonucleases.

Nucleic Acids Res.

Czapinska H, Siwek W, Szczepanowski RH, Bujnicki JM, Bochtler M, Skowronek KJ.

Crystal Structure and Directed Evolution of Specificity of NlaIV Restriction Endonuclease.

J Mol Biol.

Mitkowski P, Jagielska E, Nowak E, Bujnicki JM, Stefaniak F, Niedziałek D, Bochtler M, Sabała I.

Structural bases of peptidoglycan recognition by lysostaphin SH3b domain.

Sci Rep.


Bennabi I, Quéguiner I, Kolano A, Boudier T, Mailly P, Verlhac MH, Terret ME.

Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment.


Bochtler M, Mizgalska D, Veillard F, Nowak ML, Houston J, Veith P, Reynolds EC, Potempa J.

The Bacteroidetes Q-Rule: Pyroglutamate in Signal Peptidase I Substrates.

Front Microbiol.

Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V.

Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.

Nucleic Acids Res.

Stroynowska-Czerwinska A, Piasecka A, Bochtler M.

Specificity of MLL1 and TET3 CXXC domains towards naturally occurring cytosine modifications.

Biochim Biophys Acta Gene Regul Mech.

Fernandes H, Czapinska H, Grudziaz K, Bujnicki JM, Nowacka M.

Crystal structure of human Acinus RNA recognition motif domain.


Kisiala M, Copelas A, Czapinska H, Xu S, Bochtler M.

Crystal structure of the modification-dependent SRA-HNH endonuclease TagI

Nucleic Acids Res.

Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M.

Activity and structure of EcoKMcrA.

Nucleic Acids Res.

Piasecka A, Czapinska H, Vielberg MT, Szczepanowski RH, Kiefersauer R, Reed S, Groll M, Bochtler M.

The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.

J Mol Biol.


Slyvka A, Mierzejewska K, Bochtler M.

Nei-like 1 (NEIL1) excises 5-carboxylcytosine directly and stimulates TDG-mediated 5-formyl and 5-carboxylcytosine excision.

Sci Rep.

Perycz M, Krwawicz J, Bochtler M.

A TALE-inspired computational screen for proteins that contain approximate tandem repeats.

PLoS One


Bochtler M, Kolano A, Xu G-L

DNA demethylation pathways: Additional players and regulators


Szychowska M, Siwek W, Pawolski D, Kazrani AA, Pyrc K, Bochtler M

Type III CRISPR complexes from Thermus thermophilus

Acta Biochim Pol

Bochtler M., Piasecka A

Haloferax volcanii UbaA, catalytic engine for sampylation and sulfur transfer


Mierzejewska K, Bochtler M, Czapinska H

On the role of steric clashes in methylation control of restriction endonuclease activity

Nucleic Acids Res

Haniewicz P, Floris D, Farci D, Kirkpatrick J, Loi MC, Büchel C, Bochtler M, Piano D

Isolation of Plant Photosystem II Complexes by Fractional Solubilization

Front Plant Sci


Grabowska M, Jagielska E, Czapinska H, Bochtler M, Sabala I

High resolution structure of an M23 peptidase with a substrate analogue

Sci Rep

Burmistrz M, Dudek B, Staniec D, Rodriguez Martinez JI, Bochtler M, Potempa J, Pyrc K

Functional Analysis of Porphyromonas gingivalis W83 CRISPR-Cas Systems

J Bacteriol


Gallagher JM, Yamak A, Kirilenko P, Black S, Bochtler M, Lefebvre C, Nemer M, Latinkić BV

Carboxy terminus of GATA4 transcription factor is required for its cardiogenic activity and interaction with CDK4

Mech Dev

Sabala I, Jagielska E, Bardelang PT, Czapinska H, Dahms SO, Sharpe JA, James R, Than ME, Thomas NR, Bochtler M

Crystal structure of the antimicrobial peptidase lysostaphin from Staphylococcus simulans


Mierzejewska K, Siwek W, Czapinska H, Kaus-Drobek M, Radlinska M, Skowronek K, Bujnicki JM, Dadlez M, Bochtler M

Structural basis of the methylation specificity of R.DpnI

Nucleic Acids Res.

Wojciechowski M, Rafalski D, Kucharski R, Misztal K, Maleszka J, Bochtler M, Maleszka R

Insights into DNA hydroxymethylation in the honeybee from in-depth analyses of TET dioxygenase

Open Biol

Kazrani AA, Kowalska M, Czapinska H, Bochtler M

Crystal structure of the 5hmC specific endonuclease PvuRts1I

Nucleic Acids Res.

Jaremko M, Jaremko L, Nowakowski M, Wojciechowski M, Szczepanowski RH, Panecka R, Zhukov I, Bochtler M, Ejchart A

NMR structural studies of the first catalytic half-domain of ubiquitin activating enzyme

J Struct Biol


Haniewicz P, De Sanctis D, Büchel C, Schröder WP, Loi MC, Kieselbach T, Bochtler M, Piano D

Isolation of monomeric photosystem II that retains the subunit PsbS

Photosynth Res

Wojciechowski M, Czapinska H, Bochtler M

CpG Underrepresentation and the Bacterial CpG Specific DNA Methyltransferase M.MpeI

Proc Natl Acad Sci U S A.


Bochtler M

Structural basis of the TAL effector-DNA interaction

Biol Chem

Sabala I, Jonsson IM, Tarkowski A, Bochtler M

Anti-staphylococcal activities of lysostaphin and LytM catalytic domain

BMC Microbiol

Siwek W, Czapinska H, Bochtler M, Bujnicki JM, Skowronek K

Crystal structure and mechanism of action of the N6-methyladenine dependent type IIM restriction endonuclease

Nucleic Acids Res

Chojnowski G, Bujnicki JM, Bochtler M

RIBER/DIBER: a software suite for crystal content analysis in the studies of protein-nucleic acid complexes



Antonczak AK, Simova Z, Yonemoto IT, Bochtler M, Piasecka A, Czapinska H, Brancale A, Tippmann EM

Importance of single molecular determinants in the fidelity of expanded genetic codes

Proc Natl Acad Sci U S A.

Braun S, Humphreys C, Fraser E, Brancale A, Bochtler M, Dale TC

Amyloid-associated nucleic acid hybridisation

PLoS One

Firczuk M, Wojciechowski M, Czapinska H, Bochtler M

DNA intercalation without flipping in the specific ThaI-DNA complex

Nucleic Acids Res

Sokolowska M, Czapinska H, Bochtler M

Hpy188I-DNA pre- and post-cleavage complexes--snapshots of the GIY-YIG nuclease mediated catalysis

Nucleic Acids Res


Chojnowski G, Bochtler M

DIBER: protein, DNA or both?

Acta Crystallogr D Biol Crystallogr

Gentsch M, Kaczmarczyk A, van Leeuwen K, de Boer M, Kaus-Drobek M, Dagher MC, Kaiser P, Arkwright PD, Gahr M, Rösen-Wolff A, Bochtler M, Secord E, Britto-Williams P, Saifi GM, Maddalena A, Dbaibo G, Bustamante J, Casanova JL, Roos D, Roesler J

Alu-repeat-induced deletions within the NCF2 gene causing p67-phox-deficient chronic granulomatous disease (CGD)

Hum Mutat

Chojnowski G, Breer K, Narczyk M, Wielgus-Kutrowska B, Czapinska H, Hashimoto M, Hikishima S, Yokomatsu T, Bochtler M, Girstun A, Staroń K, Bzowska A

1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state

Biochem Biophys Res Commun

Piano D, El Alaoui S, Korza HJ, Filipek R, Sabala I, Haniewicz P, Buechel C, De Sanctis D, Bochtler M

Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotiana tabacum

Photosynth Res


Sokolowska M, Czapinska H, Bochtler M

Crystal structure of the beta beta alpha-Me type II restriction endonuclease Hpy99I with target DNA

Nucleic Acids Res


Szczepanowski RH, Carpenter MA, Czapinska H, Zaremba M, Tamulaitis G, Siksnys V, Bhagwat AS, Bochtler M

Central base pair flipping and discrimination by PspGI

Nucleic Acids Res

Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V

How PspGI, catalytic domain of EcoRII and Ecl18kI acquire specificities for different DNA targets

Nucleic Acids Res

Breer K, Wielgus-Kutrowska B, Hashimoto M, Hikishima S, Yokomatsu T, Szczepanowski RH, Bochtler M, Girstun A, Starón K, Bzowska A

Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors

Nucleic Acids Symp Ser (Oxf)

Sukackaite R, Grazulis S, Bochtler M, Siksnys V.

The recognition domain of the BpuJI restriction endonuclease in complex with cognate DNA at 1.3-A resolution

J Mol Biol

Lipka M, Filipek R, Bochtler M

Crystal structure and mechanism of the Staphylococcus cohnii virginiamycin B lyase (Vgb)



Bochtler M, Chojnowski G

The highest reflection intensity in a resolution shell

Acta Crystallogr A

Chojnowski G, Bochtler M

The statistics of the highest E value

Acta Crystallogr A

Firczuk M, Bochtler M

Folds and activities of peptidoglycan amidases

FEMS Microbiol Rev

Firczuk M, Bochtler M

Mutational analysis of peptidoglycan amidase MepA


Kaus-Drobek M, Czapinska H, Sokołowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M

Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically

Nucleic Acids Res

Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Siksnys V, Bochtler M

Restriction endonucleases that resemble a component of the bacterial DNA repair machinery

Cell Mol Life Sci

Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M

Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.

J Mol Biol

Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V

Nucleotide flipping by restriction enzymes analyzed by 2-aminopurine steady-state fluorescence

Nucleic Acids Res


Bochtler M, Szczepanowski RH, Tamulaitis G, Grazulis S, Czapinska H, Manakova E, Siksnys V

Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease



Azim MK, Goehring W, Song HK, Ramachandran R, Bochtler M, Goettig P

Characterization of the HslU chaperone affinity for HslV protease

Protein Sci

Dandanell G,Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M

Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene

J Mol Biol

Filipek R, Potempa J, Bochtler M

A comparison of staphostatin B with standard mechanism serine protease inhibitors

J Biol Chem

Firczuk M, Mucha A, Bochtler M

Crystal structures of active LytM

J Mol Biol

Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V

Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.

Proc Natl Acad Sci U S A

Groll M, Bochtler M, Brandstetter H, Clausen T, Huber R

Molecular machines for protein degradation.


Korza HJ, Bochtler M

Pseudomonas aeruginosa LD-carboxypeptidase, a serine peptidase with a Ser-His-Glu triad and a nucleophilic elbow.

J Biol Chem

Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M

Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan

J Biol Chem

Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M

Substrate access to the active sites in aminopeptidase T, a representative of a new metallopeptidase clan.

J Mol Biol

Szczepanowski RH, Filipek R, Bochtler M

Crystal structure of a fragment of mouse ubiquitin-activating enzyme.

J Biol Chem

Potempa J, Golonka E, Filipek R, Shaw LN

Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases

Mol Microbiol

Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M

Peptydoglycan amidase MepA is a LAS metallopeptidase

J Biol Chem


Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M

Peptidoglycan amidase MepA is a LAS metallopeptidase.

J Biol Chem

Bochtler M, Odintsov SG, Marcyjaniak M, Sabala I

Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases.

Protein Sci

Filipek R, Szczepanowski R, Sabat A, Potempa J, Bochtler M

Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition


Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M

Peptidoglycan amidase MepA is a LAS metallopeptidase

J Biol Chem

Odintsov SG, Sabala I, Marcyjaniak M, Bochtler M

Latent LytM at 1.3A resolution

J Mol Biol

Golonka E, Filipek R, Sabat A, Sinczak A, Potempa J

Genetic characterization of staphopain genes in Staphylococcus aureus

Biol Chem


Dubin G, Krajewski M, Popowicz G, Stec-Niemczyk J, Bochtler M, Potempa J, Dubin A, Holak TA

A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus.


Filipek R, Rzychon M, Oleksy A, Gruca M, Dubin A, Potempa J, Bochtler M

The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease.

J Biol Chem

Rzychon M, Filipek R, Sabat A, Kosowska K, Dubin A, Potempa J, Bochtler M

Staphostatins resemble lipocalins, not cystatins in fold

Protein Sci


Ramachandran R, Hartmann C, Song HK, Huber R, Bochtler M

Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY)

Proc Natl Acad Sci U S A


Palczewska M, Groves P, Ambrus A, Kaleta A, Kövér KE, Batta G, Kuźnicki J.

Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93)

Eur J Biochem.