Education and Degrees:
2014  Professor of Biological Sciences, nomination by the President of the Republic of Poland
2008  DSc Habil, Institute of Biochemistry and Biophysics, Warsaw, Poland
2000  PhD in Biochemistry, Institute of Biochemistry and Biophysics, Warsaw
1993  Master Degree in Molecular Biology, University of Warsaw
1988-1993  Biology, University of Warsaw, Poland


Awards:
2016 Award from the Premiere for the scientific achievements
2016 Elected EMBO member
2016 Laureate together with Peter Rehling of Nicolaus Copernicus Polish-German Research Award given jointly by the Foundation for Polish Science (FNP)
         and the German Research Foundation (DFG)
2015 Award from the Minister of Science and Higher Education for scientific achievements that led to the title of Professor
2015 Award from the President of Polish Academy of Science for scientific achievement
2010  EMBO Installation Grant
2009  Welcome Programme, Foundation for Polish Science
2008  Eugen-Graetz Prize for Research, University of Freiburg, Germany
2001-2003  Long-term FEBS fellowship
2001  Award for PhD thesis, Institute of Biochemistry and Biophysics, Warsaw
1997  Grant for young scientists, Polish State Committee for Scientific Research
1996  Short-term FEBS fellowship


Research experience and appointments:
2015 – present Deputy Director of Development at the International Institute of Molecular and Cell Biology in Warsaw, Poland
2009 - present  Professor and Leader of Laboratory of Mitochondrial Biogenesis, International Institute of Molecular and Cell Biology, Warsaw, Poland
2008-2009  Associate Member of Excellence Cluster BIOSS, Centre for Biological Signalling Studies, University of Freiburg, Germany
2007-2009  Member of the Board, Collaborative Research Centre (SFB 746)
2007-2010  Project Leader in Collaborative Research Centre (SFB 746), Germany
2004-2009  Group Leader (German equivalent of Assistant Professor), Institute for  Biochemistry and Molecular Biology, University of Freiburg, Germany
2001-2004  Postdoctoral Fellow, Laboratory of Prof. Nikolaus Pfanner, University of Freiburg, Germany
1999  Visiting Scientist, Laboratory of Prof. Sabine Rospert, Max Planck Research Unit, Halle, Germany
1997  Visiting Scientist, Laboratory of Prof. Gottfried Schatz, Biozentrum, University of Basel, Switzerland
1994-2000  Doctoral research with Prof. Magdalena Boguta, Institute of Biochemistry and Biophysics, Warsaw, Poland

 
Agnieszka Chacinska's profile in Science:
http://sciencecareers.sciencemag.org/career_magazine/previous_issues/articles/2010_03_19/caredit.a1000027

 

For recent publications see Research Focus and Publications

  
Pubmed search for Chacinska A

 

Selected publications from my group in Freiburg:

 

Chacinska, A., Koehler, C.M., Milenkovic, D., Lithgow, T., Pfanner, N. (2009) Importing mitochondrial proteins: machineries and mechanisms. Cell 138, 628-644. 

Milenkovic, D., Ramming, T., Müller, J.M., Wenz, L.S., Gebert, N., Schulze-Specking, A., Stojanovski, D., Rospert, S., Chacinska, A.  (2009) Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria. Mol. Biol. Cell 20, 2530-2539.

Stojanovski, D., Milenkovic, D., Müller, J.M., Gabriel, K., Schulze-Specking, A., Baker, M.J., Ryan, M.T., Guiard, B., Pfanner, N., Chacinska, A. (2008) Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase. J. Cell Biol. 183, 195-202.

Müller, J.M., Milenkovic, D., Guiard, B., Pfanner, N., Chacinska A. (2008) Precursor oxidation by Mia40 and Erv1 promotes vectorial transport of proteins into the mitochondrial intermembrane space. Mol. Biol Cell. 19, 226-236.

Stojanovski, D., Müller, JM, Milenkovic, D., Guiard, B., Pfanner, N., Chacinska, A. (2008) The MIA system for protein import into the mitochondrial intermembrane space. Biochim. Biophys. Acta, 1783, 610-617.

Milenkovic, D., Gabriel, K., Guiard, B., Schulze-Specking, A., Pfanner, N., Chacinska, A. (2007) Biogenesis of the essential Tim9-Tim10 chaperone complex of mitochondria: site-specific recognition of cysteine residues by the intermemembrane space receptor Mia40. J. Biol. Chem. 282, 22472-22480.

Wiedemann, N., Pfanner, N., and Chacinska, A. (2006). Chaperoning through the mitochondrial intermembrane space. Mol. Cell 21, 145-148.

Rissler, M., Wiedemann, N., Pfannschmidt, S., Gabriel, K., Guiard, B., Pfanner, N., and Chacinska, A. (2005). The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins. J. Mol. Biol. 353, 485-492.

Selected publications arising from my post-doctoral research:

Chacinska, A.*, Lind, M.*, Frazier, A.E., Dudek, J., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2005). Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829 (*equal contribution).

van der Laan, M.*, Chacinska, A.*, Lind, M., Perschil, I., Sickmann, A., Meyer, H.E., Guiard, B., Meisinger, C., Pfanner, N., and Rehling, P. (2005). Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol. Cell. Biol. 25, 7449-7458 (*equal contribution).

Chacinska, A., Pfannschmidt, S., Wiedemann, N., Kozjak, V., Sanjuán Szklarz, L.K., Schulze-Specking, A., Truscott, K.N., Guiard, B., Meisinger, C., and Pfanner, N. (2004). Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J. 23, 3735-3746.

Chacinska, A., Rehling, P., Guiard, B., Frazier, A.E., Schulze-Specking, A., Pfanner, N., Voos, W., and Meisinger, C. (2003). Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 22, 5370-5381.

Geissler, A.*, Chacinska, A.*, Truscott, K.N., Wiedemann, N., Brandner, K., Sickmann, A., Meyer, H.E., Meisinger, C., Pfanner, N., and Rehling, P. (2002). The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111, 507-518 (*equal contribution).

Other important contributions:

Meinecke, M., Wagner, R., Kovermann, P., Guiard, B., Mick, D.U., Hutu, D.P., Voos, W., Truscott, K.N., Chacinska, A., Pfanner, N., and Rehling, P. (2006). Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 312, 1523-1526.

Meisinger, C., Rissler, M., Chacinska, A., Sanjuán Szklarz, L.K., Milenkovic, D., Kozjak, V., Schönfisch, B., Lohaus, C., Meyer, H.E., Yaffe, M.P., Guiard, B., Wiedemann, N., and Pfanner, N. (2004). The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane. Dev. Cell 7, 61-71.

Sickmann, A., Reinders, J., Wagner, Y., Joppich, C. Zahedi, R., Meyer, H.E., Schönfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., and Meisinger, C. (2003). The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. USA 100, 13207-13212.

Wiedemann, N., Kozjak, V., Chacinska, A., Schönfisch, B., Rospert, S., Ryan, M.T., Pfanner, N., and Meisinger, C. (2003). Machinery for protein sorting and assembly in the mitochondrial outer membrane. Nature 424, 565-571.

The most important paper from my PhD studies:

Chacinska A., Boguta M., Krzewska J. and Rospert S. (2000) Prion-dependent switching between respiratory competence and deficiency in the yeast nam9-1 mutant. Mol. Cell. Biol. 20, 7220-9.

Description of Current Research

   Mitochondria play an important role in metabolism and regulatory processes in the cell. Thus, the formation of mitochondria is essential for cellular function in the entire eukaryotic kingdom, from unicellular organisms to mammals. Mitochondria comprise 1000-1500 cellular proteins, which are synthesized outside of the mitochondria in the cytosol.

   The biogenesis of mitochondria relies on the efficient import, sorting, and maturation of proteins governed by conserved protein translocases and other complex machineries. We identified a novel mitochondrial intermembrane space assembly (MIA) pathway that utilizes the transfer of disulfide bonds and is dedicated to the import and biogenesis of intermembrane space proteins that lack a classical mitochondrial leader sequence.

Our research explores novel and exciting links between disulfide bond formation mechanisms and mitochondrial protein homeostasis. We postulate the presence of unique mechanisms involved in protein biogenesis that involve crosstalk between the cytosol and mitochondrial compartments. Our research addresses three major and related issues:
•    Redox-related biogenesis events of mitochondrial proteins in yeast and higher eukaryotes.
•    The impact of the MIA pathway on mitochondrial and cellular protein homeostasis.
•    The biological consequences of oxidative protein biogenesis failure.

Our goal is to better understand the complex and dynamic processes involved in the formation of functional organelles, the maintenance of mitochondrial protein homeostasis, and their failure, which results in pathology.

 

We are always interested in qualified and highly motivated students and postdocs.  Please inquire: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Our recent publication:

Wrobel L, Topf U, Bragoszewski P , Wiese S, Sztolsztener ME , Oeljeklaus S, Varabyova A, Lirski M, Chroscicki P, Mroczek S, Januszewicz E, Dziembowski A, Koblowska M, Warscheid B, Chacinska A. Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol. 2015. Nature, 524(7566), 485-8.
 

UPRam_scheme

images/labs/chacinska/banner_2014_2e_v2-01.png
 
 
Lab Leader:Laboratory of Mitochondrial Biogenesis
Agnieszka Chacińska, PhD, Professor
This email address is being protected from spambots. You need JavaScript enabled to view it.
+48 22 59 70 779
 
Lab and Office Manager:
Maria Łepkowska, BSc
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+48 22 59 70 605

Postdoctoral Fellows:
Piotr Brągoszewski, PhD
Katarzyna Chojnacka, PhD
Łukasz Samluk, PhD
Anna Sokół, PhD
Ulrike Topf, PhD
Michał Wasilewski, PhD
Michal Turek, PhD
Paulina Sakowska, PhD

PhD Students:
Magdalena Chojnacka, MSci
Piotr Chrościcki, MSc
Łukasz Kowalski, MSc
Karthik Mohanraj, MSc
Maria Śladowska, MSci
Anna Antosiewicz, MSc
Martyna Pietrzyk, Msci

FishMed Research Assistant:
Michał Bazała, MSc

Research Technician:
Elżbieta Grzelak

Sabbatical Professor:
Dr. Carlo Vascotto

Former students and co-workers of Laboratory of Mitochondrial Biogenesis:
Arianna Barchiesi, MSc
Anita Brewińska, MSc
Tomasz Czerwik, MSc
Magdalena Długołęcka, MSci
Beata Drabarek, PhD
Aleksandra Fergin, MSc
Edyta Głów, MSc
Agnieszka Górnicka, PhD
Elżbieta Januszewicz, PhD
Magdalena Kaus-Drobek, PhD
Adrianna Łoniewska-Lwowska, PhD
Aleksandra Matusiak, MSci
Inmaculada Mora Espi, MSc
Kamila Ornoch, MSc
Małgorzata Sztolsztener, PhD
Krzysztof Tarasiuk, MSci
Agata Trojanowska, MSc
Aksana Varabyova, PhD
Jakub Dominowski, MSci
Lidia Wróbel, PhD
Sabine Poerschke

 

 

2019

Cytosolic translational responses differ under conditions of severe short-term and long-term mitochondrial stress.

Samluk L, Urbanska M, Kisielewska K, Mohanraj K, Kim MJ, Machnicka K, Liszewska E, Jaworski J, Chacinska A.

Mol Biol Cell.. 2019

Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins.

Monteuuis G, Miścicka A, Świrski M, Zenad L, Niemitalo O, Wrobel L, Alam J, Chacinska A, Kastaniotis AJ, Kufel J.

Nucleic Acids Res.. 2019

Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.

Mohanraj K, Wasilewski M, Benincá C, Cysewski D, Poznanski J, Sakowska P, Bugajska Z, Deckers M, Dennerlein S, Fernandez-Vizarra E, Rehling P, Dadlez M, Zeviani M, Chacinska A.

EMBO Mol Med.. 2019

2018

Quantitative proteomics identifies redox switches for global translation modulation by mitochondrially produced reactive oxygen species.

Topf U, Suppanz I, Samluk L, Wrobel L, Böser A, Sakowska P, Knapp B, Pietrzyk MK, Chacinska A, Warscheid B.

Nat Commun. . 2018

Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins.

Kowalski L, Bragoszewski P, Khmelinskii A, Glow E, Knop M, Chacinska A.

BMC Biol. . 2018

Loss of the Mia40a oxidoreductase leads to hepato-pancreatic insufficiency in zebrafish.

Sokol AM, Uszczynska-Ratajczak B, Collins MM, Bazala M, Topf U, Lundegaard PR, Sugunan S, Guenther S, Kuenne C, Graumann J, Chan SSL, Stainier DYR, Chacinska A.

PLoS Genet.. 2018

2017

Control of mitochondrial biogenesis and function by the ubiquitin-proteasome system

Bragoszewski P, Turek M, Chacinska A.

Open Biol. 2017

Cell biology: Sort and destroy

Chacinska A.

Nature. 2017

Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography.

Gold VA, Chroscicki P, Bragoszewski P, Chacinska A.

EMBO Rep.. 2017

2016

Chatty Mitochondria: Keeping Balance in Cellular Protein Homeostasis

Topf U, Wrobel L, Chacinska A

Trends Cell Biol. 2016

The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly

Wrobel L, Sokol AM, Chojnacka M, Chacinska A

Sci Rep. 2016

Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential

Turakhiya U, von der Malsburg K, Gold VA, Guiard B, Chacinska A, van der Laan M, Ieva R

J Mol Biol. 2016

TIM29 is a subunit of the human carrier translocase required for protein transport

Callegari S, Richter F, Chojnacka K, Jans DC, Lorenzi I, Pacheu-Grau D, Jakobs S, Lenz C, Urlaub H, Dudek J, Chacinska A, Rehling P

FEBS Lett. 2016

Białka mitochondrialne – import, eksport, degradacja

Wasilewski M, Chojnacka K, Chacinska A

Postępy Biochemii. 2016

Protein trafficking at the crossroads to mitochondria

Wasilewski M, Chojnacka K, Chacinska A

Biochim Biophys Acta. 2016

2015

Mitochondrial translocation of APE1 relies on the MIA pathway

Barchiesi A, Wasilewski M, Chacinska A, Tell G, Vascotto C

Nucleic Acids Res. 2015

Cox17 is an auxiliary factor involved in the control of the mitochondrial contact site and cristae organizing system

Chojnacka M, Gornicka A, Oeljeklaus S, Warscheid B, Chacinska A

J Biol Chem. 2015

Retro-translocation of mitochondrial intermembrane space proteins

Bragoszewski P, Wasilewski M, Sakowska P, Gornicka A, Böttinger L, Qiu J, Wiedemann N, Chacinska A

Proc Natl Acad Sci U S A. 2015

Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol

Wrobel L, Topf U, Bragoszewski P , Wiese S, Sztolsztener ME , Oeljeklaus S, Varabyova A, Lirski M, Chroscicki P, Mroczek S, Januszewicz E, Dziembowski A, Koblowska M, Warscheid B, Chacinska A

Nature. 2015

The oxidation status of Mic19 regulates MICOS assembly

Sakowska P, Jans DC, Mohanraj K, Riedel D, Jakobs S, Chacinska A

Mol Cell Biol. 2015

Glucocerebrosidase 1 deficient Danio rerio mirror key pathological aspects of human Gaucher disease and provide evidence of early microglial activation preceding alpha-synuclein-independent neuronal cell death

Keatinge M, Bui H, Menke A, Chen YC, Sokol AM, Bai Q, Ellett F, Da Costa M, Burke D, Gegg M, Trollope L, Payne T, McTighe A, Mortiboys H, de Jager S, Nuthall H, Kuo MS, Fleming A, Schapira AH, Renshaw SA, Highley JR, Chacinska A, Panula P, Burton EA, O'Neill MJ, Bandmann O

Hum Mol Genet. 2015

2014

Mgr2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane

Ieva R, Schrempp SG, Opaliński L Wollweber F, Höß P, Heißwolf AK, Gebert M, Zhang Y, Guiard B, Rospert S, Becker T, Chacinska A, Pfanner N, van der Laan M

Mol Cell.. 2014

Uniform nomenclature for the mitochondrial contact site and cristae organizing system

Pfanner N, van der Laan M, Amati P, Capaldi RA, Caudy AA, Chacinska A, Darshi M, Deckers M, Hoppins S, Icho T, Jakobs S, Ji J, Kozjak-Pavlovic V, Meisinger C, Odgren PR, Park SK, Rehling P, Reichert AS, Sheikh MS, Taylor SS, Tsuchida N, van der Bliek AM, van der Klei IJ, Weissman JS, Westermann B, Zha J, Neupert W, Nunnari J

J Cell Biol.. 2014

Mitochondrial protein translocases for survival and wellbeing

Sokol AM, Sztolsztener ME, Wasilewski M, Heinz E, Chacinska A

FEBS Lett. 2014

Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix

Melin J, Schulz C, Wrobel L, Bernhard O, Chacinska A, Jahn O, Schmidt B, Rehling P

Mol Cell Biol. 2014

A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria

Gornicka A, Bragoszewski P, Chroscicki P, Wenz LS, Schulz C, Rehling P, Chacinska A

Mol Biol Cell. 2014

2013

Disulfide bond formation: sulfhydryl oxidase ALR controls mitochondrial biogenesis of human MIA40

Sztolsztener ME, Brewinska A, Guiard B, Chacinska A

Traffic. 2013

Mitochondrial protein homeostasis

Varabyova A, Stojanovski D, Chacinska A

IUBMB Life. 2013

Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria

Wrobel L, Trojanowska A, Sztolsztener ME, Chacinska A

Mol Biol Cell. 2013

The ubiquitin-proteasome system regulates mitochondrial intermembrane space proteins

Bragoszewski P, Gornicka A, Sztolsztener ME, Chacinska A

Mol Cell Biol. 2013

Mia40 and MINOS act in parallel with Ccs1 in the biogenesis of mitochondrial Sod1

Varabyova A, Topf U, Kwiatkowska P, Wrobel L, Kaus-Drobek M, Chacinska A

FEBS J. 2013

Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation

Qiu J, Wenz LS, Zerbes RM, Oeljeklaus S, Bohnert M, Stroud DA, Wirth C, Ellenrieder L, Thornton N, Kutik S, Wiese S, Schulze-Specking A, Zufall N, Chacinska A, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N, Becker T

Cell. 2013

2012

The MIA pathway: A tight bond between protein transport and oxidative folding in mitochondria

Stojanovski D, Bragoszewski P, Chacinska A

BBA-MOL CELL RES. 2012

Role of MINOS in protein biogenesis of the mitochondrial outer membrane

Bohnert M, Wenz LS, Zerbes RM, Horvath SE, Stroud DA, von der Malsburg K, Müller JM, Oeljeklaus S, Perschil I, Warscheid B, Chacinska A, Veenhuis M, van der Klei IJ, Daum G, Wiedemann N, Becker T, Pfanner N, van der Laan M

Mol Biol Cell.. 2012

Intermembrane space proteome of yeast mitochondria

Voegtle FN, Burkhart JM, Rao S, Gerbeth C, Hinrichs J, Martinou JC, Chacinska A, Sickmann A, Zahedi RP, Meisinger C

Mol Cell Proteomics. 2012

In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins

Böttinger L*, Gornicka A*, Czerwik T, Bragoszewski P, Loniewska-Lwowska A, Schulze-Specking A, Truscott KN, Guiard B, Milenkovic D, Chacinska A, *equally contributing first author

Mol Biol Cell. 2012

2011

The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins

Becker T, Wenz LS, Krüger V, Lehmann W, Müller JM, Goroncy L, Zufall N, Lithgow T, Guiard B, Chacinska A, Wagner R, Meisinger C, Pfanner N

J Cell Biol.. 2011

Dual role of mitofilin in mitochondrial membrane organization and protein biogenesis

von der Malsburg K, Müller JM, Bohnert M, Oeljeklaus S, Kwiatkowska P, Becker T, Loniewska-Lwowska A, Wiese S, Rao S, Milenkovic D, Hutu DP, Zerbes RM, Schulze-Specking A, Meyer HE, Martinou JC, Rospert S, Rehling P, Meisinger C, Veenhuis M et all

Dev Cell.. 2011

Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex

Schulz C, Lytovchenko O, Melin J, Chacinska A, Guiard B, Neumann P, Ficner R, Jahn O, Schmidt B, Rehling P

J Cell Biol.. 2011

A high density consensus map of rye (Secale cereale L.) based on DArT markers

Milczarski P, Bolibok-Brągoszewska H, Myśków B, Stojałowski S, Heller-Uszyńska K, Góralska M, Brągoszewski P, Uszyński G, Kilian A, Rakoczy-Trojanowska M

PLoS One. 2011