- Badania
- Laboratorium Biologii Strukturalnej
Laboratorium Biologii Strukturalnej
2021
Bochtler M, Fernandes H.
DNA adenine methylation in eukaryotes: Enzymatic mark or a form of DNA damage?
Pastor M, Czapinska H, Helbrecht I, Krakowska K, Lutz T, Xu S, Bochtler M.
Crystal structures of the EVE-HNH endonuclease VcaM4I in the presence and absence of DNA.
Czapinska H, Winiewska-Szajewska M, Szymaniec-Rutkowska A, Piasecka A, Bochtler M, Poznański J.
Halogen Atoms in the Protein-Ligand System. Structural and Thermodynamic Studies of the Binding of Bromobenzotriazoles by the Catalytic Subunit of Human Protein Kinase CK2.
Bochtler M.
Distinction between self and non-self in restriction modification: The mysterious case of type IIL enzymes.
2020
Bochtler M.
Arrhenius-law-governed homo- and heteroduplex dissociation.
Skowronek KJ, Bochtler M.
In Vitro Directed Evolution of a Restriction Endonuclease With More Stringent Specificity.
Kisiala M, Kowalska M, Pastor M, Korza HJ, Czapinska H, Bochtler M.
Restriction endonucleases that cleave RNA/DNAheteroduplexes bind dsDNA in A-like conformation.
Lutz T, Czapinska H, Fomenkov A, Potapov V, Heiter DF, Cao B, Dedon P, Bochtler M, Xu S.
Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases.
Fricke T, Smalakyte D, Lapinski M, Pateria A, Weige C, Pastor M, Kolano A, Winata C, Siksnys V, Tamulaitis G, Bochtler M.
Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish.
Xu G-L, Bochtler M.
Reversal of nucleobase methylation by dioxygenases.
2019
Mitkowski P, Jagielska E, Nowak E, Bujnicki JM, Stefaniak F, Niedziałek D, Bochtler M, Sabała I.
Structural bases of peptidoglycan recognition by lysostaphin SH3b domain.
Czapinska H, Siwek W, Szczepanowski RH, Bujnicki JM, Bochtler M, Skowronek KJ.
Crystal Structure and Directed Evolution of Specificity of NlaIV Restriction Endonuclease.
Lutz T, Flodman K, Copelas A, Czapinska H, Mabuchi M, Fomenkov A, He X, Bochtler M, Xu S.
A protein architecture guided screen for modification dependent restriction endonucleases.
Slyvka A, Zagorskaitė E, Czapinska H, Sasnauskas G, Bochtler M.
Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping.
2018
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M.
Activity and structure of EcoKMcrA.
Kisiala M, Copelas A, Czapinska H, Xu S, Bochtler M.
Crystal structure of the modification-dependent SRA-HNH endonuclease TagI
Fernandes H, Czapinska H, Grudziaz K, Bujnicki JM, Nowacka M.
Crystal structure of human Acinus RNA recognition motif domain.
Stroynowska-Czerwinska A, Piasecka A, Bochtler M.
Specificity of MLL1 and TET3 CXXC domains towards naturally occurring cytosine modifications.
Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V.
Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.
Bochtler M, Mizgalska D, Veillard F, Nowak ML, Houston J, Veith P, Reynolds EC, Potempa J.
The Bacteroidetes Q-Rule: Pyroglutamate in Signal Peptidase I Substrates.
Bennabi I, Quéguiner I, Kolano A, Boudier T, Mailly P, Verlhac MH, Terret ME.
Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment.
Piasecka A, Czapinska H, Vielberg MT, Szczepanowski RH, Kiefersauer R, Reed S, Groll M, Bochtler M.
The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.
2017
Perycz M, Krwawicz J, Bochtler M.
A TALE-inspired computational screen for proteins that contain approximate tandem repeats.
Slyvka A, Mierzejewska K, Bochtler M.
Nei-like 1 (NEIL1) excises 5-carboxylcytosine directly and stimulates TDG-mediated 5-formyl and 5-carboxylcytosine excision.
2016
Haniewicz P, Floris D, Farci D, Kirkpatrick J, Loi MC, Büchel C, Bochtler M, Piano D
Isolation of Plant Photosystem II Complexes by Fractional Solubilization
Mierzejewska K, Bochtler M, Czapinska H
On the role of steric clashes in methylation control of restriction endonuclease activity
Bochtler M., Piasecka A
Haloferax volcanii UbaA, catalytic engine for sampylation and sulfur transfer
Szychowska M, Siwek W, Pawolski D, Kazrani AA, Pyrc K, Bochtler M
Type III CRISPR complexes from Thermus thermophilus
Bochtler M, Kolano A, Xu G-L
DNA demethylation pathways: Additional players and regulators
2015
Burmistrz M, Dudek B, Staniec D, Rodriguez Martinez JI, Bochtler M, Potempa J, Pyrc K
Functional Analysis of Porphyromonas gingivalis W83 CRISPR-Cas Systems
Grabowska M, Jagielska E, Czapinska H, Bochtler M, Sabala I
High resolution structure of an M23 peptidase with a substrate analogue
2014
Gallagher JM, Yamak A, Kirilenko P, Black S, Bochtler M, Lefebvre C, Nemer M, Latinkić BV
Carboxy terminus of GATA4 transcription factor is required for its cardiogenic activity and interaction with CDK4
Jaremko M, Jaremko L, Nowakowski M, Wojciechowski M, Szczepanowski RH, Panecka R, Zhukov I, Bochtler M, Ejchart A
NMR structural studies of the first catalytic half-domain of ubiquitin activating enzyme
Kazrani AA, Kowalska M, Czapinska H, Bochtler M
Crystal structure of the 5hmC specific endonuclease PvuRts1I
Wojciechowski M, Rafalski D, Kucharski R, Misztal K, Maleszka J, Bochtler M, Maleszka R
Insights into DNA hydroxymethylation in the honeybee from in-depth analyses of TET dioxygenase
Mierzejewska K, Siwek W, Czapinska H, Kaus-Drobek M, Radlinska M, Skowronek K, Bujnicki JM, Dadlez M, Bochtler M
Structural basis of the methylation specificity of R.DpnI
Sabala I, Jagielska E, Bardelang PT, Czapinska H, Dahms SO, Sharpe JA, James R, Than ME, Thomas NR, Bochtler M
Crystal structure of the antimicrobial peptidase lysostaphin from Staphylococcus simulans
2013
Wojciechowski M, Czapinska H, Bochtler M
CpG Underrepresentation and the Bacterial CpG Specific DNA Methyltransferase M.MpeI
Haniewicz P, De Sanctis D, Büchel C, Schröder WP, Loi MC, Kieselbach T, Bochtler M, Piano D
Isolation of monomeric photosystem II that retains the subunit PsbS
2012
Chojnowski G, Bujnicki JM, Bochtler M
RIBER/DIBER: a software suite for crystal content analysis in the studies of protein-nucleic acid complexes
Siwek W, Czapinska H, Bochtler M, Bujnicki JM, Skowronek K
Crystal structure and mechanism of action of the N6-methyladenine dependent type IIM restriction endonuclease
Sabala I, Jonsson IM, Tarkowski A, Bochtler M
Anti-staphylococcal activities of lysostaphin and LytM catalytic domain
Bochtler M
Structural basis of the TAL effector-DNA interaction
2011
Sokolowska M, Czapinska H, Bochtler M
Hpy188I-DNA pre- and post-cleavage complexes--snapshots of the GIY-YIG nuclease mediated catalysis
Braun S, Humphreys C, Fraser E, Brancale A, Bochtler M, Dale TC
Amyloid-associated nucleic acid hybridisation
Antonczak AK, Simova Z, Yonemoto IT, Bochtler M, Piasecka A, Czapinska H, Brancale A, Tippmann EM
Importance of single molecular determinants in the fidelity of expanded genetic codes
Firczuk M, Wojciechowski M, Czapinska H, Bochtler M
DNA intercalation without flipping in the specific ThaI-DNA complex
2010
Piano D, El Alaoui S, Korza HJ, Filipek R, Sabala I, Haniewicz P, Buechel C, De Sanctis D, Bochtler M
Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotiana tabacum
Chojnowski G, Breer K, Narczyk M, Wielgus-Kutrowska B, Czapinska H, Hashimoto M, Hikishima S, Yokomatsu T, Bochtler M, Girstun A, Staroń K, Bzowska A
1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state
Gentsch M, Kaczmarczyk A, van Leeuwen K, de Boer M, Kaus-Drobek M, Dagher MC, Kaiser P, Arkwright PD, Gahr M, Rösen-Wolff A, Bochtler M, Secord E, Britto-Williams P, Saifi GM, Maddalena A, Dbaibo G, Bustamante J, Casanova JL, Roos D, Roesler J
Alu-repeat-induced deletions within the NCF2 gene causing p67-phox-deficient chronic granulomatous disease (CGD)
Chojnowski G, Bochtler M
DIBER: protein, DNA or both?
2009
Sokolowska M, Czapinska H, Bochtler M
Crystal structure of the beta beta alpha-Me type II restriction endonuclease Hpy99I with target DNA
2008
Lipka M, Filipek R, Bochtler M
Crystal structure and mechanism of the Staphylococcus cohnii virginiamycin B lyase (Vgb)
Sukackaite R, Grazulis S, Bochtler M, Siksnys V.
The recognition domain of the BpuJI restriction endonuclease in complex with cognate DNA at 1.3-A resolution
Breer K, Wielgus-Kutrowska B, Hashimoto M, Hikishima S, Yokomatsu T, Szczepanowski RH, Bochtler M, Girstun A, Starón K, Bzowska A
Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors
Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V
How PspGI, catalytic domain of EcoRII and Ecl18kI acquire specificities for different DNA targets
Szczepanowski RH, Carpenter MA, Czapinska H, Zaremba M, Tamulaitis G, Siksnys V, Bhagwat AS, Bochtler M
Central base pair flipping and discrimination by PspGI
2007
Tamulaitis G, Zaremba M, Szczepanowski RH, Bochtler M, Siksnys V
Nucleotide flipping by restriction enzymes analyzed by 2-aminopurine steady-state fluorescence
Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M
Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.
Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Siksnys V, Bochtler M
Restriction endonucleases that resemble a component of the bacterial DNA repair machinery
Kaus-Drobek M, Czapinska H, Sokołowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M
Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically
Firczuk M, Bochtler M
Mutational analysis of peptidoglycan amidase MepA
Firczuk M, Bochtler M
Folds and activities of peptidoglycan amidases
Chojnowski G, Bochtler M
The statistics of the highest E value
Bochtler M, Chojnowski G
The highest reflection intensity in a resolution shell
2006
Bochtler M, Szczepanowski RH, Tamulaitis G, Grazulis S, Czapinska H, Manakova E, Siksnys V
Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease
2005
Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M
Peptydoglycan amidase MepA is a LAS metallopeptidase
Potempa J, Golonka E, Filipek R, Shaw LN
Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases
Szczepanowski RH, Filipek R, Bochtler M
Crystal structure of a fragment of mouse ubiquitin-activating enzyme.
Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M
Substrate access to the active sites in aminopeptidase T, a representative of a new metallopeptidase clan.
Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M
Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan
Korza HJ, Bochtler M
Pseudomonas aeruginosa LD-carboxypeptidase, a serine peptidase with a Ser-His-Glu triad and a nucleophilic elbow.
Groll M, Bochtler M, Brandstetter H, Clausen T, Huber R
Molecular machines for protein degradation.
Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V
Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.
Firczuk M, Mucha A, Bochtler M
Crystal structures of active LytM
Filipek R, Potempa J, Bochtler M
A comparison of staphostatin B with standard mechanism serine protease inhibitors
Dandanell G,Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M
Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene
Azim MK, Goehring W, Song HK, Ramachandran R, Bochtler M, Goettig P
Characterization of the HslU chaperone affinity for HslV protease
2004
Golonka E, Filipek R, Sabat A, Sinczak A, Potempa J
Genetic characterization of staphopain genes in Staphylococcus aureus
Odintsov SG, Sabala I, Marcyjaniak M, Bochtler M
Latent LytM at 1.3A resolution
Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M
Peptidoglycan amidase MepA is a LAS metallopeptidase
Filipek R, Szczepanowski R, Sabat A, Potempa J, Bochtler M
Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition
Bochtler M, Odintsov SG, Marcyjaniak M, Sabala I
Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases.
Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M
Peptidoglycan amidase MepA is a LAS metallopeptidase.
2003
Rzychon M, Filipek R, Sabat A, Kosowska K, Dubin A, Potempa J, Bochtler M
Staphostatins resemble lipocalins, not cystatins in fold
Filipek R, Rzychon M, Oleksy A, Gruca M, Dubin A, Potempa J, Bochtler M
The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease.
Dubin G, Krajewski M, Popowicz G, Stec-Niemczyk J, Bochtler M, Potempa J, Dubin A, Holak TA
A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus.
2002
Ramachandran R, Hartmann C, Song HK, Huber R, Bochtler M
Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY)
2001
Palczewska M, Groves P, Ambrus A, Kaleta A, Kövér KE, Batta G, Kuźnicki J.
Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93)