- Badania
- Laboratorium Struktury Białka
Laboratorium Struktury Białka
2022
Labedzka-Dmoch K, Razew M, Gapinska M, Piatkowski J, Kolondra A, Salmonowicz H, Wenda JM, Nowotny M, Golik P.
The Pet127 protein is a mitochondrial 5'-to-3' exoribonuclease from the PD-(D/E)XK superfamily involved in RNA maturation and intron degradation in yeasts.
Corona A, Wycisk K, Talarico C, Manelfi C, Milia J, Cannalire R, Esposito F, Gribbon P, Zaliani A, Iaconis D, Beccari AR, Summa V, Nowotny M, Tramontano E.
Natural Compounds Inhibit SARS-CoV-2 nsp13 Unwinding and ATPase Enzyme Activities.
2021
Warminski M, Kowalska J, Nowak E, Kubacka D, Tibble R, Kasprzyk R, Sikorski PJ, Gross JD, Nowotny M, Jemielity J.
Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect.
Nowacka M, Nowak E, Czarnocki-Cieciura M, Jackiewicz J, Skowronek K, Szczepanowski R, Wöhrl BM, Nowotny M.
Structures of substrate complexes of foamy viral protease-reverse transcriptase.
Das A, Thapa P, Santiago U, Shanmugam N, Banasiak K, Dabrowska K, Nolte H, Szulc NA, Gathungu RM, Cysewski D, Krüger M, Dadlez M, Nowotny M, Camacho CJ, Hoppe T, Pokrzywa W.
Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity.
2020
Jaciuk M, Swuec P, Gaur V, Kasprzak JM, Renault L, Dobrychłop M, Nirwal S, Bujnicki JM, Costa A, Nowotny M.
A combined structural and biochemical approach reveals translocation and stalling of UvrB on the DNA lesion as a mechanism of damage verification in bacterial nucleotide excision repair.
Hyjek-Składanowska M, Vickers T, Napiórkowska A, Anderson B, Tanowitz M, Crooke ST, Liang XH, Seth PP, Nowotny M.
Origins of the increased affinity of phosphorothioate-modified therapeutic nucleic acids for proteins.
Szewczyk M, Malik D, Borowski LS, Czarnomska SD, Kotrys AV, Klosowska-Kosicka K, Nowotny M, Szczesny RJ.
Human REXO2 Controls Short Mitochondrial RNAs Generated by mtRNA Processing and Decay Machinery to Prevent Accumulation of Double-Stranded RNA.
Kaus-Drobek M, Mücke N, Szczepanowski RH, Wedig T, Czarnocki-Cieciura M, Polakowska M, Herrmann H, Wysłouch-Cieszyńska A, Dadlez M.
Vimentin S-glutathionylation at Cys328 Inhibits Filament Elongation and Induces Severing of Mature Filaments in Vitro.
Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine..
Kuciński J, Chamera S, Kmera A, Rowley MJ, Fujii S, Khurana P, Nowotny M, Wierzbicki AT.
Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana.
Hyjek-Składanowska M, Stasińska AR, Napiórkowska-Gromadzka A, Bartłomiejczak A, Seth PP, Chmielewski MK, Nowotny M.
Disulfide bridge cross-linking between protein and the RNA backbone as a tool to study RNase H1.
Koralewski R, Dymek B, Mazur M, Sklepkiewicz P, Olejniczak S, Czestkowski W, Matyszewski K, Andryianau G, Niedziejko P, Kowalski M, Gruza M, Borek B, Jedrzejczak K, Bartoszewicz A, Pluta E, Rymaszewska A, Kania M, Rejczak T, Piasecka S, Mlacki M, Mazurkiewicz M, Piotrowicz M, Salamon M, Zagozdzon A, Napiorkowska-Gromadzka A, Bartlomiejczak A, Mozga W, Dobrzański P, Dzwonek K, Golab J, Nowotny M, Olczak J, Golebiowski A.
Discovery of OATD-01, a First-in-Class Chitinase Inhibitor as Potential New Therapeutics for Idiopathic Pulmonary Fibrosis.
Cieśla M, Turowski TW, Nowotny M, Tollervey D, Boguta M.
The expression of Rpb10, a small subunit common to RNA polymerases, is modulated by the R3H domain-containing Rbs1 protein and the Upf1 helicase.
2019
Hyjek M, Figiel M, Nowotny M.
RNases H: Structure and mechanism.
Górecka KM, Krepl M, Szlachcic A, Poznański J, Šponer J, Nowotny M.
RuvC uses dynamic probing of the Holliday junction substrate to achieve sequence specificity and efficient resolution.
Gaur V, Ziajko W, Nirwal S, Szlachcic A, Gapińska M, Nowotny M
Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease.
Nowotny M.
Crosslink and shield: protecting abasic sites from error-prone repair.
2018
Figiel M, Krepl M, Park S, Poznański J, Skowronek K, Gołąb A, Ha T, Šponer J, Nowotny M.
Mechanism of polypurine tract primer generation by HIV-1 reverse transcriptase.
Razew M, Warkocki Z, Taube M, Kolondra A, Czarnocki-Cieciura M, Nowak E, Labedzka-Dmoch K, Kawinska A, Piatkowski J, Golik P, Kozak M, Dziembowski A, Nowotny M.
Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.
Wojtczak BA, Sikorski PJ, Fac-Dabrowska K, Nowicka A, Warminski M, Kubacka D, Nowak E, Nowotny M, Kowalska J, Jemielity J.
5′-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes.
2017
Figiel M, Krepl M, Poznanski J, Golab A, Šponer J, Nowotny M
Coordination between the polymerase and RNase H activity of HIV-1 reverse transcriptase
Figiel M, Nowotny M
Chapter Six - Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein–Nucleic Acid Complexes
Rydzik AM, Warminski M, Sikorski PJ, Baranowski MR, Walczak S, Kowalska J, Zuberek J, Lukaszewicz M, Nowak E, W Claridge TD, Darzynkiewicz E, Nowotny M, Jemielity J.
mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation.
Rydzik AM, Warminski M, Sikorski PJ, Baranowski MR, Walczak S, Kowalska J, Zuberek J, Lukaszewicz M, Nowak E, W Claridge TD, Darzynkiewicz E, Nowotny M, Jemielity J.
mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation.
Charbonnel C, Niazi AK, Elvira-Matelot E, Nowak E, Zytnicki M, de Bures A, Jobet E, Opsomer A, Shamandi N, Nowotny M, Carapito C, Reichheld J-P, Vaucheret H, Saez-Vasquez J.
The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.
2016
Nowotny M, Gaur V
Structure and mechanism of nucleases regulated by SLX4
Stracy M, Jaciuk M, Uphoff S, Kapanidis AN, Nowotny M, Sherratt DJ, Zawadzki P
Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli
Kalisiak K, Kuliński TM, Tomecki R, Cysewski D, Pietras Z, Chlebowski A, Kowalska K, Dziembowski A
A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans
2015
Gaur V, Wyatt HDM, Komorowska W, Szczepanowski RH, de Sanctis D, Gorecka KM, West SC, Nowotny M
Structural and Mechanistic Analysis of the Slx1-Slx4 Endonuclease
Roszczenko P, Grzeszczuk M, Kobierecka P, Wywial E, Urbanowicz P, Wincek P, Nowak E, Jagusztyn-Krynicka EK
Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231
Bocian-Ostrzycka KM, Łasica AM, Dunin-Horkawicz S, Grzeszczuk MJ, Drabik K, Dobosz AM, Godlewska R, Nowak E, Collet J-F, Jagusztyn-Krynicka EK
Functional and evolutionary analyses of Helicobacter pylori HP0231 (DsbK) protein with strong oxidative and chaperone activity characterized by a highly diverged dimerization domain
2014
Smietanski M, Werner M, Purta E, Kaminska KH, Stepinski J, Darzynkiewicz E, Nowotny M, Bujnicki JM
Structural analysis of human 2′-O-ribose methyltransferases involved in mRNA cap structure formation
Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SFJ, Nowotny M
Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry
Tomecki R, Drazkowska K, Kucinski I, Stodus K, Szczesny RJ, Gruchota J, Owczarek EP, Kalisiak K, Dziembowski A
Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target
Majorek KA, Dunin-Horkawicz S, Steczkiewicz K, Muszewska A, Nowotny M, Ginalski K, Bujnicki JM
The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification
Mathys H, Basquin J, Ozgur S, Czarnocki-Cieciura M, Bonneau F, Aartse A, Dziembowski A, Nowotny M, Conti E, Filipowicz W.
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
Figiel M, Nowotny M.
Crystal structure of RNase H3-substrate complex reveals parallel evolution of RNA/DNA hybrid recognition.
Miętus M, Nowak E, Jaciuk M, Kustosz P, Studnicka J, Nowotny M
Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding
2013
Nowak E, Potrzebowski W, Konarev PV, Rausch JW, Bona MK, Svergun DI, Bujnicki JM, Le Grice SF, Nowotny M
Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid
Chon H, Sparks JL, Rychlik M, Nowotny M, Burgers PM, Crouch RJ, Cerritelli SM
RNase H2 roles in genome integrity revealed by unlinking its activities
Górecka KM, Komorowska W, Nowotny M
Crystal structure of RuvC resolvase in complex with Holliday junction substrate
2012
Sulej AA, Tuszynska I, Skowronek KJ, Nowotny M, Bujnicki JM
Sequence-specific cleavage of the RNA strand in DNA-RNA hybrids by the fusion of ribonuclease H with a zinc finger
2011
Rosta E, Nowotny M, Yang W, Hummer G
Catalytic mechanism of RNA backbone cleavage by ribonuclease H from quantum mechanics/molecular mechanics simulations
Figiel M, Chon H, Cerritelli SM, Cybulska M, Crouch RJ, Nowotny M
The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects
Jaciuk M, Nowak E, Skowronek K, Tańska A, Nowotny M
Structure of UvrA nucleotide excision repair protein in complex with modified DNA.
2010
Rychlik MP, Chon H, Cerritelli SM, Klimek P, Crouch RJ, Nowotny M
Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.
2009
Nowotny M
Retroviral integrase superfamily: the structural perspective.
Nowotny M, Yang W
Structural and functional modules in RNA interference
2004
Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ.
Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.
2003
Nowotny M, Spiechowicz M, Jastrzebska B, Filipek A, Kitagawa K, Kuznicki J
Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins
2002
Filipek A, Jastrzebska B, Nowotny M, Kuznicki J
CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family
Filipek A, Jastrzebska B, Nowotny M, Kwiatkowska K, Hetman M, Surmacz L, Wyroba E, Kuznicki J
Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells.
Filipek A, Jastrzebska B, Nowotny M, Kuznicki J
CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family
2000
Nowotny M, Bhattacharya S, Filipek A, Krezel AM, Chazin W, Kuznicki J.
Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein.