Fluorescence spectrometry can be as much as thousands of times more sensitive than absorbence techniques. This makes it possible to analyze samples in the nanogram to picogram range.
Fluorescence is used to identify or quantify specific molecules in complex mixtures. If the compound of interest is not fluorescent by itself, it can be labeled with specific fluorescent probes. The RF-5301PC‘s synchronous scanning mode allows mixtures of fluorochromes to be analyzed. Biological activity of enzymes can be assayed using substrates that yield a fluorescent product after cleavage.
The RF-5301PC is computer driven for data acquisition and processing. Its high throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital signal processing to provide an excellent signal:noise ratio. From: Shimadzu Product Information Sheet. Contact: Roman Szczepanowski, This email address is being protected from spambots. You need JavaScript enabled to view it.