Genome Engineering Unit of the International Institute of Molecular and Cell Biology is inviting applications for a (Junior) Specialist responsible for genotyping and cloning

Genome Engineering Unit is one of the Core Facilities of the International Institute of Molecular and Cell Biology. Core facilities play an integral role in the Institute’s infrastructure, providing access to state-of-the-art research equipment and services to support the daily work of researchers from the Institute and external entities. One of the key services provided by the Genome Engineering Unit are genotyping of mouse lines generated by CRISPR/Cas9 and plasmid preparation. Key responsibilities include, but are not limited to:

• Isolation of genomic and plasmid DNA (HotShot, Miniprep, Midiprep, isolation using magnetic beads)
• Preparation of PCR reactions, post-reaction clean-up, agarose gel electrophoresis
• Restriction enzyme digestion
• Cloning using SLIC method
• Preparation of sequencing reaction using SLIC method
• Analysis of sequencing results

Requirements for the candidate:

• Holds a BSc degree in biology, biotechnology, or a related field
• English language skills allowing to communicate freely in an international environment
• Practical knowledge of molecular biology methods, especially working with DNA (DNA isolation, including DNA isolation from animal samples, PCR)
• Very good organization of work, ability to set priorities
• Practical knowledge of genome engineering methods, especially plasmid preparation methods, will be an advantage
• Snap Gene program knowledge will be an advantage

We offer:

• Civil-law contract (replacement employee), expected contract duration is 1.5 years
• Remuneration of 5200-6000 PLN per month (gross)
• Full technical, administrative, and organizational support from professional English-speaking
• Possibility to establish an individual/fixed working schedule
• Paid time off : 20/26 days (as per the Labor Code) and additional paid leave starting from the 2nd year of employment
• 13th salary
• Work in a friendly environment in a leading research institution in Poland
• Co-financing of the Multisport card
• Attractive social package
• Private medical insurance

How to apply: 

• Apply now to This email address is being protected from spambots. You need JavaScript enabled to view it.; In the subject include "Specialist_GEU"
• Deadline for applications is 10th October 2023
• Your application should contain a CV and a response to the following question:

Using restriction enzymes, plan an experiment to cut the GFP coding sequence from Addgene #47516 plasmid. Please provide:

• Names and catalog numbers of enzymes used
• Reaction composition and incubation time to digest 1 ug of plasmid (plasmid concentration: 250 ng/ul)
• Expected band sizes after electrophoresis in agarose gel

Please include the following statement in your CV: “I hereby agree to the processing of my personal data, included in the application documents by the International Institute of Molecular and Cell Biology in Warsaw, 4 Księcia Trojdena Street, 02-109 Warsaw, for the purpose of carrying out the current recruitment process.” Your personal data will be processed for the purpose of the recruitment procedure by the International Institute of Molecular and Cell Biology in Warsaw. Full information is available under the link information clause  https://bit.ly/3UFWpY2.
The competition may be extended until a suitable candidate who fulfils all requirements is found.

Chcielibyśmy poinformować, że konkurs na stanowisko Specjalista ds. genotypowania został rozstrzygnięty. Wybranym kandydatem jest Apolonia Witecka./We would like to inform that the competition for Specialist responsible for genotyping and cloning has been closed. The selected candidate is Apolonia Witecka.