DESCRIPTION OF CURRENT RESEARCH

We are interested in the molecular mechanisms that are involved in neurodegeneration, with a special emphasis on the role of Ca2+ homeostasis and signaling. These processes are being studied at the genomic, proteomic, and cellular levels using mostly zebrafish, rats, and mice as model organisms. The projects are focused on proteins that are involved in store-operated Ca2+ entry (SOCE) and Ca2+ homeostasis in mitochondria, the involvement of potassium channels in the brain ventricular system, and the in vivo analysis of Ca2+ homeostasis in neurons using zebrafish models. For recent reviews, see Wegierski and Kuznicki (Cell Calcium, 2018) and Winata and Korzh (FEBS Lett, 2018).

ROLE OF STIM PROTEINS IN STORE-OPERATED Ca2+ ENTRY IN NEURONS

We previously showed that STIM1 is involved in a thapsigargin-induced SOCE-like process, whereas STIM2 is mostly active after the EGTA-driven depletion of extracellular Ca2+ (Gruszczynska-Biegala et al., PLoS One, 2011; Gruszczynska-Biegala and Kuznicki, J Neurochem, 2013). We searched for new partners of STIMs other than ORAI channels and found that endogenous STIMs associate with GluA subunits of AMPA receptors (Gruszczynska-Biegala et al., Front Cell Neurosci, 2016). STIM proteins also associate in vitro with NMDA receptors. The results suggest cross-talk between STIM proteins and NMDA receptors and their effect on Ca2+ influx through NMDA receptors (Gruszczynska-Biegala et al., Cells, 2020). Using zebrafish as a model we study STIM2 functions in vivo. We evaluated the expression of Calcium Toolkit genes in zebrafish brain and established the level of SOCE-components (Wasilewska et al., Genes, 2019). We generated stim2a, stim2b, and stim2a:2b knock-out zebrafish lines and analyze them using in vivo calcium imaging in cytosols and mitochondria, behavioral tests and scRNA-Seq.

DYSREGULATION OF Ca2+ HOMEOSTASIS IN COMMON NEURODEGENERATIVE DISEASES

We have been testing the hypothesis that brain dysfunction during aging is induced by changes in Ca2+ homeostasis, which may predispose the brain to SAD pathologies. Transgenic mice that overexpressed key SOCE proteins (STIM1, STIM2, and ORAI1) specifically in brain neurons under the Thy1 promoter were generated. Characterization of the STIM1 line (Majewski et al., BBA Mol Cell Res, 2017, Gruszczynska-Biegała et. al., Cells, 2020), STIM2/ORAI1 (Majewski et al., IJMS, 2020) and ORAI1 lines (Maciag et al., BBA Mol Cell Res, 2019; Majewski et. al., IJMS, 2019) has been reported. Strikingly, aged transgenic ORAI1 mice developed spontaneous seizure-like events that could be observed only in females, suggesting a novel, sex-dependent role of ORAI1 in neural function (Maciag, et al., BBA-MCR 2019). Furthermore, based on RNAseq gene expression profiling analysis and ddPCR conducted on hippocampus we identified a downregulation of Arx gene. The loss of function mutations of ARX are known to be implicated in human cases of epilepsy (Majewski et al., IJMS, 2019).

FAD mutations in presenilins were shown to alter both endoplasmic reticulum (ER) calcium signaling and SOCE, but the role of amyloid precursor protein (APP) and APP FAD mutants in intracellular calcium homeostasis is controversial. Our data indicate that APP and APP FAD mutants are not directly involved in SOCE (Wegierski et al., Biochem Biophys Res Commun, 2016). Instead, we found that APP knockdown resulted in an elevation of the resting levels of ER Ca2+, reduced Ca2+ leakage, and delayed the translocation of STIM1 to Orai1 upon ER Ca2+ store depletion. Our data suggest a regulatory role for APP in ER Ca2+ (Gazda et al., Sci Rep, 2017).

Using quantitative PCR, we compared microRNA (miRNA) profiles in blood plasma from mild cognitive impairment-AD patients (whose diagnoses were confirmed by cerebrospinal fluid [CSF] biomarkers) with AD patients and non-demented, age-matched controls. We adhered to standardized blood and CSF assays that are recommended by the JPND BIOMARKAPD consortium. Six miRNAs (three not yet reported in the context of AD and three reported in AD blood) were selected as the most promising biomarker candidates that can differentiate early AD from controls with the highest fold changes (Nagaraj et al., Oncotarget, 2017; patent pending: PCT/IB2016/052440).

A loss-of-function mutation of PINK1 causes early-onset Parkinson’s disease in humans. In collaboration with Oliver Bandmann (University of Sheffield), we used a pink1 mutant (pink1-/-) zebrafish line to study alterations of Ca2+ homeostasis (Flinn et al., Ann Neurol, 2013; Soman et al., Eur J Neurosci, 2016). We generated mcu knock-out fish, which is viable and fertile. The pink1-/-/mcu-/- double-knockout line shows no loss of dopaminergic neurons, suggesting that Ca2+ that enters mitochondria via the mitochondrial Ca2+ uniporter is involved in the pathology of the pink1 mutant. We expressed a mitochondrial Ca2+ probe (CEPIA2mt) under a pan-neuronal promoter (elavl3) to visualize Ca2+ levels in the mitochondrial matrix of a zebrafish (Tg[elavl3:CEPIA2mt]). Lightsheet fluorescence microscopy enabled us to visualize chemically inducible Ca2+ flux in zebrafish neurons in vivo. Inactivation of mitochondrial calcium uniporter (mcu) is protective in both genetic and chemical models of Parkinson disease (Soman et al., Biol Open. 2019). Hence, regulating the mcu function could be an effective therapeutic target in managing pathological changes in the Parkinson disease.

RARE AND ULTRA-RARE DISEASES WITH NEUROLOGICAL PRESENTATION

We previously showed that a mutation of huntingtin (HTT) in YAC128 mice (i.e., a model of Huntington’s disease) resulted in the higher expression of some components of the calcium signalosome, including huntingtin-associated protein 1 (HAP1) and calcyclin (S100A6) binding protein (CacyBP/SIP; Czeredys et al., Front Mol Neurosci, 2013). We detected greater activity of SOC channels in medium spiny neurons (MSNs) from YAC128 mice and found that some tetrahydrocarbazoles restored the disturbances in Ca2+ homeostasis and stabilized SOCE in YAC128 MSN cultures (Czeredys et al., Biochem Biophys Res Commun, 2017). The overexpression of HAP1 protein isoform A in MSNs from YAC128 mice was found to enhance the activity of SOC channels after the activation of IP3R1 (Czeredys at al., Front Cell Neurosci, 2018).

We also study Ca2+ homeostasis and its impact on the progression of neurodegeneration, behavioral changes, seizures, and sleep disturbances in several rare neurodegenerative diseases affecting children. Using CRISPR-Cas9 technology, we have created zebrafish lines with a mutation in the npc2, sgsh, ppp3ca, ptpn4a, and ptpn4b genes. We use those lines for identification of new diagnostic markers for early diagnosis and monitoring progression of two lysosomal storage diseases - the Niemann-Pick type C (NPC), and mucopolisaccharidosis type III (MPS-III), as well better understanding mechanisms leading to pathological changes in two ultra-rare conditions affecting just a few children world-wide (Rydzanicz et al., Eur J Hum Genet., 2018; Szczałuba et al., Clin Genet, 2019). The Zebrafish model is especially suited for drug screens. As NPC and MPS-III share many futures with Alzheimer's disease, hence drug targets that will be identified might find application in the management of a broad range of common neurodegenerative diseases.

DEVELOPMENT OF HOLLOW ORGANS

Subunits of the voltage-gated potassium channels Kcnb1 (Kv2.1) and Kcng4 (Kv6.4) are expressed in several hollow organs (e.g., brain ventricular system [BVS], ear, and eye) where they form tetrameric K+ channels and antagonize each other’s activity. The deficiency of Kcnb1 in zebrafish causes microcephaly, and the gain-of-function of Kcnb1 causes hydrocephalus. Kcng4 acts in a reverse manner (Shen et al., Sci Rep, 2016). The deficiency of BVS in human causes epilepsy (Jedrychowska and Korzh, Dev Dynam, 2019). Formation of the BVS occurs during the early neural development of vertebrates (Korzh, Cell Mol Life Sci, 2018). Deficiency of the BVS has been linked to numerous neurodegenerative diseases. Formation of the BVS depends on many factors, including the ependyma (i.e., cells that line the BVS cavity) and circumventricular organs, including the choroid plexus (Garcia-Lecea et al., Front Neuroanat, 2017; Korzh and Kondrychyn, Semin Cell Dev Biol, 2019). To study the role of K+ channels in the development of hollow organs, we generated a mutant of Kcnb1 and two mutants of kcng4b with deficiency in the BVS and ear. To distinguish the mechanism of action of KCNB1 mutations in human we initiated an analysis of developmental defects caused by mRNA of these mutants in the developing brain and ear of zebrafish

 jkuznicki

Jacek Kuźnicki, PhD, Professor 

Correspondence address:
Laboratory of Neurodegeneration
International Institute of Molecular and Cell Biology
4 Ks. Trojdena Street, 02-109 Warsaw, Poland
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

DEGREES

1993 - Professor, awarded by the President of the Republic of Poland
1987 - DSc. Habil., Nencki Institute of Experimental Biology PAS, Warsaw, Poland
1980 - PhD in biochemistry, Nencki Institute of Experimental Biology PAS, Warsaw, Poland
1976 - MSc in biochemistry, Faculty of Biology, University of Warsaw, Poland

PROFESSIONAL EXPERIENCE

2001-present - Professor, Head of Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland
2020-2022 - President of the National Science Centre Council, member since 2018
2001-2018 - Director, International Institute of Molecular and Cell Biology in Warsaw, Poland; Feb-Dec 2018 Acting Director, International Institute of Molecular and Cell Biology in Warsaw, Poland
2017-2018 - Deputy Chair of the Council of Provosts, 2nd Division: Biological and Agricultural Sciences, Polish Academy of Sciences
2000-2001 - Director, Centre of Excellence Phare Sci-Tech II, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1999-2001 - Acting Director, International Institute of Molecular and Cell Biology in Warsaw, Poland; Organizer and Director, Centenarian Program
1996-2002 - Head, Laboratory of Calcium Binding Proteins, professor 2002- 2014 Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1992-1995 - Visiting Professor, NIMH, Bethesda, MD, USA
1991-1992 - Deputy Scientific Director, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1986-1992 - Associate Professor and Head of Laboratory of Calcium Binding Proteins, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1984-1985 - Research Associate, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1981-1984 - Visiting Fellow, NIH, Bethesda, MD, USA
1980-1981 - Post-doctoral Fellow, Nencki Institute
1976-1980 - PhD Student, Nencki Institute of Experimental Biology PAN, Warsaw

PROFESSIONAL TRAINING

2018 - Visiting Professor, Laboratory of H. Burgess, National Institute of Child Health and Human Development, Bethesda, MD, USA
2015 - Visiting Professor, Laboratory of W. Harris, University of Cambridge, UK 
2014 - Visiting Professor, Laboratory of B.E. Snaar-Jagalska, Leiden University, The Netherlands
1992-1995 - Visiting Professor, Laboratory of D. Jacobowitz, National Institute of Mental Health, Bethesda, MD, USA
1981-1984 - Visiting Fellow (postdoc), Laboratory of E.D. Korn, National Institute of Heart, Lung and Blood, Bethesda, MD, USA

MEMBERSHIP IN SCIENTIFIC SOCIETIES, ORGANIZATIONS, AND PANELS

2020-present - External expert in biotechnology, Łukasiewcz Research Network - PORT Polish Center for Technology Development
2020-present
- Ordinary Member, Polish Academy of Sciences
2017-2018 - Deputy Chair, Council of Provosts, 2nd Division, Polish Academy of Sciences
2016-present - Member, International Advisory Board, Małopolska Centre of Biotechnology, Jagiellonian University
2011-present - Member, International Expert Council of the Research and Education Center, State Key Laboratory of Molecular and Cellular Biology, Ukraine
2011-2014 - Member, Science Policy Committee, and Rotating President (Jul-Dec 2012), Ministry of Science and Higher Education
2008-present - Board Member, European Calcium Society
2008-2018 - Member, Board of Directors, and Rotating President (Jul-Dec 2016, Jul-Dec 2013, Jul-Dec 2010), Biocentrum-Ochota Consortium
2006-2011 - Member, Advisory Group, 7FP HEALTH, European Commission
2004-2019 - Corresponding Member, Polish Academy of Sciences
2004-present - Honorary Chair and co-founder, BioEducation Foundation
2002-present - Head of Program Board, Centre for Innovative Bioscience Education
1993-2014 - Member, Scientific Council, Nencki Institute of Experimental Biology, Polish Academy of Sciences
1996-1998 & 2000-2002 - Vice-President, Biotechnology Committee, Polish Academy of Sciences
1989 - 1991 - General Secretary, Polish Biochemical Society

HONORS, PRIZES AND AWARDS

2018 - Commemorative medal for the 100th Anniversary of the Nencki Institute for outstanding contribution to the development of this Institute, supporting its activities, shaping the image and building a mutual success
2013 - Crystal Brussels Prize for outstanding achievements in 7th Framework Programme of the European Union for Research and Development
2013 - Award from the 2nd Division of Biological and Agricultural Sciences, Polish Academy of Sciences for series of works on β-catenin
2011 - Konorski Award for the best Polish research work in neurobiology awarded by the Polish Neuroscience Society and Committee on Neurobiology of the PAS, Warsaw
2008 - Officer's Cross of the Order of Polonia Restituta awarded by the President of Poland
2004-2007 - Professorial Subsidy Program Award from Foundation for Polish Science
2003 - Prime Minister Award for the scientific achievements, Warsaw
2001 - Award from the Division of Biological Sciences of the PAS for the work on CaBP
1998 - Knight's Cross of the Order of Polonia Restituta awarded by the President of  Poland
1987 - Polish Anatomical Society Award for the article in “Advances in Cell Biology”
1986 - Skarzynski Award from the Polish Biochemical Society for the best review article in “Advances in Biochemistry”, Warsaw
1977 - Mozolowski Award, Polish Biochem. Society for outstanding Polish young biochemist
1977 - Parnas Award from Polish Biochem. Society for the best paper in biochemical research
1976 - MSc, Magna cum laude, University of Warsaw

DOCTORATES DEFENDED UNDER LAB LEADER’S SUPERVISION

A. Filipek, J. Kordowska, U. Wojda, J. Hetman, M. Palczewska, M. Nowotny, K. Billing-Marczak, Ł. Bojarski, W. Michowski, K. Misztal,    M. Figiel, K. Honarnejad, A. Jaworska, K. Gazda, F. Maciąg, J. Jędrychowska, I. Wasilewska.

LN team 2021

Lab Leader
Jacek Kuźnicki, PhD, Professor

Senior Researchers
Magdalena Czeredys, PhD
Małgorzata Korzeniowska (formerly Wiweger), PhD
Vladimir Korzh, PhD
Łukasz Majewski, PhD

Research Specialist
Sergii Palchevskyi, PhD (part-time)
Iga Wasilewska, PhD

PhD Students
Rishikesh Kumar Gupta, MSc Tech
Ewelina Latoszek, MSc Eng.

Volunteers
Justyna Jędrychowska, PhD
Monika Kwiatkowska, MSc Eng.
Anna Sarosiak, MSc

Lab Technician
Monika Matuszczyk (part-time)

Laboratory Support Specialist
Dominika Dubicka-Boroch, MSc

 

2022

Higher ATM expression in lymphoblastoid cell lines from centenarian compared with younger women.

Hadar A, Voinsky I, Parkhomenko O, Puzianowska-Kuźnicka M, Kuźnicki J, Gozes I, Gurwitz D.

Drug Dev Res. . 2022

Loss of Unconventional Myosin VI Affects cAMP/PKA Signaling in Hindlimb Skeletal Muscle in an Age-Dependent Manner.

Lehka L, Wojton D, Topolewska M, Chumak V, Majewski Ł, Rędowicz MJ.

Front Physiol. 2022

Generation of three human iPSC lines from patients with Huntington’s disease with different CAG lengths and human control iPSC line from a healthy donor.

Latoszek E, Piechota M, Liszewska E, Hansíkova ́H, Klempír J,
Mühlback A, Landwehrmeyer GB, Kuznicki J, Czeredys M.

Stem Cell Res.. 2022

Siah‑1‑interacting protein regulates mutated huntingtin protein aggregation in Huntington’s disease models.

Latoszek E, Wiweger M, Ludwiczak J, Dunin‑Horkawicz S, Kuznicki J, Czeredys M.

Cell & Bioscience. 2022

Benchtop X-band electron paramagnetic resonance detection of melanin and Nitroxyl spin probe in zebrafish.

Makarova K, Zawada K, Wiweger M.

Free Radical Biology and Medicine. 2022

2021

Camel regulates development of the brain ventricular system.

Yang S, Emelyanov A, You MS, Sin M, Korzh V.

Cell Tissue Res.. 2021

An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique.

Gasanov EV, Jędrychowska J, Pastor M, Wiweger M, Methner A, Korzh VP.

Mol Biol Rep.. 2021

Kcnb1 plays a role in development of the inner ear.

Jedrychowska J, Gasanov EV, Korzh V.

Dev Biol.. 2021

Molecular Components of Store-Operated Calcium Channels in the Regulation of Neural Stem Cell Physiology, Neurogenesis, and the Pathology of Huntington’s Disease.

Latoszek E, Czeredys M.

Front Cell Dev Biol.. 2021

Evolutionary context can clarify gene names: Teleosts as a case study.

Gasanov EV, Jędrychowska J, Kuźnicki J, Korzh V.

BioEssays. 2021

npc2-Deficient Zebrafish Reproduce Neurological and Inflammatory Symptoms of Niemann-Pick Type C Disease.

Wiweger M, Majewski L, Adamek-Urbanska D, Wasilewska I, Kuznicki J.

Front Cell Neurosci.. 2021

Caffeine-Cyclodextrin Complexes as Solids: Synthesis, Biological and Physicochemical Characterization.

Szmeja S, Gubica T, Ostrowski A, Zalewska A, Szeleszczuk Ł, Zawada K, Zielińska-Pisklak M, Skowronek K, Wiweger M.

Int J Mol Sci.. 2021

The development of zebrafish pancreas affected by deficiency of Hedgehog signaling.

Korzh S, Winata CL, Gong Z, Korzh V.

Gene Expr Patterns.. 2021

High behavioural variability mediated by altered neuronal excitability in auts2 mutant zebrafish.

Jha U, Kondrychyn I, Korzh V, Thirumalai V.

bioRxiv. 2021

Annexin A3: a newly identified player in store‑operated calcium entry.

Dyrda A, Kuznicki J, Majewski L.

Acta Neurobiol Exp . 2021

2020

STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons.

Gruszczynska-Biegala J, Strucinska K, Maciag F, Majewski L, Sladowska M, Kuznicki J.

Cells. 2020

Transgenic Mice Overexpressing Human STIM2 and ORAI1 in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration.

Majewski L, Maciąg F, Boguszewski PM, Kuznicki J.

Int. J. Mol. Sci.. 2020

miR-7 Controls the Dopaminergic/Oligodendroglial Fate through Wnt/β-catenin Signaling Regulation.

Adusumilli L, Facchinello N, Teh C, Busolin G, Le MT, Yang H, Beffagna G, Campanaro S, Tam WL, Argenton F, Lim B, Korzh V, Tiso N.

Cells . 2020

Targeting mitochondrial calcium pathways as a potential treatment against Parkinson’s disease.

Dey K, Bazala MA, Kuznicki J.

Cell Calcium. 2020

The effects of temperature on the proxies of visual detection of Danio rerio larvae: observations from the optic tectum.

Babkiewicz E, Bazała M, Urban P, Maszczyk P, Markowska M, Gliwicz M.

Biology Open . 2020

stim2b Knockout Induces Hyperactivity and Susceptibility to Seizures in Zebrafish Larvae.

Wasilewska I, Gupta RK, Wojtaś B, Palchevska O, Kuźnicki J.

Cells. 2020

Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing.

Gupta RK, Kuznicki J.

Cells. 2020

Origin and development of circumventricular organs in living vertebrate.

Korzh V, Kondrychyn I.

Semin Cell Dev Biol.. 2020

Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae.

Gupta RK, Wasilewska I, Palchevska O, Kuźnicki J.

Int. J. Mol. Sci.. 2020

Dysregulation of Neuronal Calcium Signaling via Store-Operated Channels in Huntington’s Disease.

Czeredys M.

Front Cell Dev Biol.. 2020

Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions.

Veerapathiran S, Teh C, Zhu S, Kartigayen I, Korzh V, Matsudaira PT, Wohland T.

eLife. 2020

Respiratory Syndrome Coronavirus Infections: Possible Mechanisms of Neurological Implications-A Systematic Review.

de Assis GG, Murawska-Cialowicz E, Cieszczyk P, Gasanov EV.

Front Neurol.. 2020

BDNF Val66Met Polymorphism, the Allele-Specific Analysis by qRT-PCR - a Novel Protocol.

de Assis GG, Hoffman JR, Gasanov EV.

Int J Med Sci.. 2020

Facultative Parthenogenesis in a Diamondback Water Snake (Nerodia rhombifer).

Gasanov EV, Katz AV.

Russ J Herpetol.. 2020

β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice.

Balatskyi VV, Palchevska OL, Bortnichuk L, Gan AM, Myronova A, Macewicz LL, Navrulin VO, Tumanovska LV, Olichwier A, Dobrzyn P, Piven OO.

Life (Basel). 2020

2019

Novel calcineurin A (PPP3CA) variant associated with epilepsy, constitutive enzyme activation and downregulation of protein expression.

Rydzanicz M, Wachowska M, Cook EC, Lisowski P, Kuźniewska B, Szymańska K, Diecke S, Prigione A, Szczałuba K, Szybińska A, Koppolu A, Murcia Pienkowski V, Kosińska J, Wiweger M, Kostrzewa G, Brzozowska M, Domańska-Pakieła D, Jurkiewicz E, Stawiński P, Gromadka A, Zielenkiewicz P, Demkow U, Dziembowska M, Kuźnicki J, Creamer TP, Płoski R.

Eur J Hum Genet.. 2019

Polyelectrolyte Membrane Scaffold Sustains Growth of Neuronal Cells.

Grzeczkowicz A, Gruszczynska-Biegala J, Czeredys M, Kwiatkowska A, Strawski M, Szklarczyk M, Koźbiał M, Kuźnicki J, Granicka LH.

J Biomed Mater Res A.. 2019

Behavioral and electrophysiological changes in female mice overexpressing ORAI1 in neurons.

Maciąg F, Majewski Ł, Boguszewski P.M, Gupta R.K, Wasilewska I, Wojtas B, Kuznicki J.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2019

Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

Wasilewska I, Gupta RK, Palchevska O, Kuźnicki J.

Genes (Basel). 2019

Glomerular permeability is not affected by heparan sulfate glycosaminoglycan deficiency in zebrafish embryos.

Khalil R, Lalai RA, Wiweger MI, Avramut CM, Koster AJ, Spaink HP, Bruijn JA, Hogendoorn PCW, Baelde HJ.

Am J Physiol Renal Physiol.. 2019

Kv2.1 voltage‐gated potassium channels in developmental perspective.

Jędrychowska J, Korzh V.

Dev Dyn.. 2019

Restriction of mitochondrial calcium overload by mcu inactivation renders neuroprotective effect in Zebrafish models of Parkinson's disease.

Soman SK, Bazała M, Keatinge M, Bandmann O, Kuznicki J.

Biol Open.. 2019

Changes in Calcium Homeostasis and Gene Expression Implicated in Epilepsy in Hippocampi of Mice Overexpressing ORAI1.

Majewski L, Wojtas B, Maciąg F, Kuznicki J.

Int J Mol Sci. . 2019

2018

Neuronal calcium signaling via store-operated channels in health and disease.

Wegierski T, Kuznicki J.

Cell Calcium.. 2018

Huntingtin-Associated Protein 1A Regulates Store-Operated Calcium Entry in Medium Spiny Neurons From Transgenic YAC128 Mice, a Model of Huntington’s Disease.

Czeredys M, Vigont VA, Boeva VA, Mikoshiba K, Kaznacheyeva EV, Kuznicki J.

Front Cell Neurosci.. 2018

Efficacy of chronic BACE1 inhibition in PS2APP mice depends on the regional Aβ deposition rate and plaque burden at treatment initiation.

Brendel M, Jaworska A, Overhoff F, Blume T, Probst F, Gildehaus FJ, Bartenstein P, Haass C, Bohrmann B, Herms J, Willem M, Rominger A.

Theranostics. 2018

Brain derived neutrophic factor, a link of aerobic metabolism to neuroplasticity.

De Assis GG, Gasanov EV, de Sousa MBC, Kozacz A, Murawska-Cialowicz E. Brain derived neutrophic factor, a link of aerobic metabolism to neuroplasticity.

J Physiol Pharmacol. . 2018

Development of brain ventricular system

Korzh V

Cell Mol Life Sci. 2018

SIRT1, miR-132 and miR-212 link human longevity to Alzheimer's Disease.

Hadar A, Milanesi E, Walczak M, Puzianowska-Kuźnicka M, Kuźnicki J, Squassina A, Niola P, Chillotti C, Attems J, Gozes I, Gurwitz D.

Sci Rep.. 2018

2017

Tetrahydrocarbazoles decrease elevated SOCE in medium spiny neurons from transgenic YAC128 mice, a model of Huntington's disease

Czeredys M, Maciag F, Methner A, Kuznicki J

Biochem Biophys Res Commun. 2017

TCF7L2 mediates the cellular and behavioral response to chronic lithium treatment in animal models

Misztal K, Brozko N, Nagalski A, Szewczyk LM, Królak M, Brzozowska K, Kuznicki J, Wisniewska MB

Neuropharmacology. 2017

Inhibition of the mitochondrial calcium uniporter (MCU) rescues dopaminergic neurons in pink1-/- zebrafish

Soman S, Keatinge M, Moein M, DaCosta M, Mortiboys H, Skupin A, Sugunan S, Bazala M, Kuznicki J, Bandmann O

Eur J Neurosci.. 2017

Increase of TREM2 during Aging of an Alzheimer’s Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis

Brendel M, Kleinberger G, Probst F, Jaworska A, Overhoff F, Blume T, Albert NL, Carlsen J, Lindner S, Gildehaus FJ, Ozmen L, Suárez-Calvet M, Bartenstein P, Baumann K, Ewers M, Herms J, Haass C, Rominger A

Front. Aging Neurosci.. 2017

Profile of 6 microRNA in blood plasma distinguish early stage Alzheimer's disease patients from non-demented subjects

Nagaraj S, Laskowska-Kaszub K, Dębski KJ, Wojsiat J, Dąbrowski M, Gabryelewicz T, Kuźnicki J, Wojda U

Oncotarget. 2017

Responsive mesoporous silica nanoparticles for sensing of hydrogen peroxide and simultaneous treatment toward heart failure

Tan SY, Teh C, Ang CY, Li M, Li P, Korzh V, Zhao Y

Nanoscale. 2017

Identification of a Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides

Koh CH, Wu J, Chung YY, Liu Z, Zhang RR, Chong K, Korzh V, Ting S, Oh S, Shim W, Tian HY, Wei H

Sci Rep.. 2017

Ikk2 regulates cytokinesis during vertebrate development

Shen H, Shin EM, Lee S, Mathavan S, Koh H, Osato M, Choi H, Tergaonkar V, Korzh V

Sci Rep. 2017

P53 Dysfunction in Neurodegenerative Diseases – The Cause or Effect of Pathological Changes?

Szybinska A, Lesniak W

Aging Dis.. 2017

Knockdown of amyloid precursor protein increases calcium levels in the endoplasmic reticulum.

Gazda K, Kuznicki J, Wegierski T.

Sci Rep. 2017

Overexpression of STIM1 in neurons in mouse brain improves contextual learning and impairs long-term depression.

Majewski Ł, Maciąg F, Boguszewski PM, Wasilewska I, Wiera G, Wójtowicz T, Mozrzymas J, Kuznicki J.

Biochim Biophys Acta. 2017

Time Courses of Cortical Glucose Metabolism and Microglial Activity Across the Life Span of Wild-Type Mice: A PET Study.

Brendel M, Focke C, Blume T, Peters F, Deussing M, Probst F, Jaworska A, Overhoff F, Albert N, Lindner S, von Ungern-Sternberg B, Bartenstein P, Haass C, Kleinberger G, Herms J, Rominger A.

J Nucl Med.. 2017

PET/CT evaluation of 18F-FDG uptake in pericoronary adipose tissue in patients with stable coronary artery disease: Independent predictor of atherosclerotic lesions' formation?

Mazurek T, Kobylecka M, Zielenkiewicz M, Kurek A, Kochman J, Filipiak KJ, Mazurek K, Huczek Z, Królicki L, Opolski G.

J Nucl Cardiol. 2017

Mikroskopowe obrazowanie jonów wapnia za pomocą genetycznie kodowanych sond (Microscopic imaging of calcium ions with genetically encoded calcium indicators).

Gazda K, Bazała M, Węgierski T.

Postepy Biochem.. 2017

ZnO@Gd2O3 core/shell nanoparticles for biomedical applications: Physicochemical, in vitro and in vivo characterization.

Woźniak A, Grześkowiak BF, Babayevska N, Zalewski T, Drobna M, Woźniak-Budych M, Wiweger M, Słomski R, Jurga S.

Mater Sci Eng C Mater Biol Appl. . 2017

Development of Circumventricular Organs in the Mirror of Zebrafish Enhancer-Trap Transgenics.

García-Lecea M, Gasanov E, Jedrychowska J, Kondrychyn I, Teh C, You MS, Korzh V.

Front Neuroanat. . 2017

2016

Molecular anatomy of the thalamic complex and the underlying transcription factors

Nagalski A, Puelles L, Dabrowski M, Wegierski T, Kuznicki J, Wisniewska MB

Brain Struct Funct. 2016

Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein

Wegierski T, Gazda K, Kuznicki J

Biochem Biophys Res Commun. 2016

AMPA Receptors Are Involved in Store-Operated Calcium Entry and Interact with STIM Proteins in Rat Primary Cortical Neurons

Gruszczynska-Biegala J, Sladowska M, Kuznicki J

Frontiers in Cellular Neuroscience. 2016

Be Healthy as a Fish Educational Program at the International Institute of Molecular and Cell Biology in Warsaw, Poland.

Goś D, Szymańska E, Białek-Wyrzykowska U, Wiweger M, Kuźnicki J.

Zebrafish. 2016

2015

SOCE in neurons: Signaling or just refilling?

Majewski L, Kuznicki J

BBA-Mol Cell Res. 2015

Amyloid-PET predicts inhibition of de novo plaque formation upon chronic γ-secretase modulator treatment

Brendel M, Jaworska A, Herms J, Trambauer J, Rötzer C, Gildehaus FJ, Carlsen J, Cumming P, Bylund J, Luebbers T, Bartenstein P, Steiner H, Haass C, Baumann K, Rominger A

Mol Psychiatry.. 2015

Research reforms: Ukrainian science needs elixir of youth.

Vassetzky Y, Gout I, Kuznicki J.

Nature. 2015

2014

Identification of tetrahydrocarbazoles as novel multifactorial drug candidates for treatment of Alzheimer's disease

Honarnejad K, Daschner A, Gehring AP, Szybinska A, Giese A, Kuznicki J, Bracher F, Herms J

Transl Psychiatry.. 2014

Cognitive Performance and Functional Status Are the Major Factors Predicting Survival of Centenarians in Poland

Mossakowska M , Broczek K, Wieczorowska-Tobis K, Klich-Raczka A, Jonas M, Pawlik-Pachucka E, Safranow K, Kuznicki J , Puzianowska-Kuznicka M

J Gerontol A Biol Sci Med Sci. 2014

Cognitive flexibility and long-term depression (LTD) are impaired following β-catenin stabilization in vivo

Mills F, Bartlett TE, Dissing-Olesen L, Wisniewska MB, Kuznicki J, Macvicar BA, Wang YT, Bamji SX

Proc Natl Acad Sci U S A.. 2014

2013

Postnatal isoform switch and protein localization of LEF1 and TCF7L2 transcription factors in cortical, thalamic, and mesencephalic regions of the adult mouse brain

Nagalski A, Irimia M, Szewczyk L, Ferran JL, Misztal K, Kuznicki J, Wisniewska MB

Brain Struct Funct. 2013

Downregulation of extracellular signal-regulated kinase 1/2 activity by calmodulin KII modulates p21Cip1 levels and survival of immortalized lymphocytes from Alzheimer's disease patients

Esteras N, Alquézar C, Bermejo-Pareja F, Bialopiotrowicz E, Wojda U, Martín-Requero A

Neurobiol Aging. 2013

Hsp90 and its co-chaperone, Sgt1, as autoantigens in dilated cardiomyopathy

Kapustian LL, Vigontina OA, Rozhko OT, Ryabenko DV, Michowski W, Lesniak W, Filipek A, Kroupskaya IV, Sidorik LL

Heart Vessels. 2013

CYR61, a matricellular protein, is needed for dendritic arborization of hippocampal neurons

Malik AR, Urbanska M, Gozdz A, Swiech LJ, Nagalski A, Perycz M, Blazejczyk M, Jaworski J

J Biol Chem. 2013

Physiological Role of β-Catenin/TCF Signaling in Neurons of the Adult Brain

Wisniewska MB

Neurochem Res. 2013

APBB2 genetic polymorphisms are associated with severe cognitive impairment in centenarians

Golanska E, Sieruta M, Gresner SM, Pfeffer A, Chodakowska-Zebrowska M, Sobow TM, Klich I, Mossakowska M, Szybinska A, Barcikowska M, Liberski PP

Exp Gerontol. 2013

Analysis of calcium homeostasis in fresh lymphocytes from patients with sporadic Alzheimer's disease or mild cognitive impairment

Jaworska A, Dzbek J, Styczynska M, Kuznicki J

Biochim Biophys Acta. 2013

The prion protein M129V polymorphism: Longevity and cognitive impairment among Polish centenarians

Golanska E, Sieruta M, Corder E, Gresner SM, Pfeffer A, Chodakowska-Zebrowska M, Sobow TM, Klich I, Mossakowska M, Szybinska A, Barcikowska M, Liberski PP

Prion. 2013

Alzheimer's disease modeling: ups, downs, and perspectives for human induced pluripotent stem cells

Wojda U, Kuznicki J

J Alzheimers Dis. 2013

Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons

Gruszczynska-Biegala J, Kuznicki J

J Neurochem. 2013

FRET-Based Calcium Imaging: A Tool for High-Throughput/Content Phenotypic Drug Screening in Alzheimer Disease

Honarnejad K, Kirsch AK, Daschner A, Szybinska A, Kuznicki J, Herms J

J Biomol Screen. 2013

Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington’s disease

Czeredys M, Gruszczynska-Biegala J, Schacht T, Methner A, Kuznicki J

Front. Mol. Neurosci. 2013

Development and Implementation of a High-Throughput Compound Screening Assay for Targeting Disrupted ER Calcium Homeostasis in Alzheimer's Disease

Honarnejad K, Daschner A, Giese A, Zall A, Schmidt B, Szybinska A, Kuznicki J, Herms J

PLoS One. 2013

Mikroskopowe metody rejestracji wewnątrzkomórkowych odpowiedzi wapniowych

Wegierski T

Kosmos. 2013

2012

Lack of Pur-alpha alters postnatal brain development and causes megalencephaly

Hokkanen S, Feldmann HM, Ding H, Jung CK, Bojarski L, Renner-Müller I, Schüller U, Kretzschmar H, Wolf E, Herms J

Hum Mol Genet.. 2012

Conformational altered p53 as an early marker of oxidative stress in Alzheimer's disease

Buizza L, Cenini G, Lanni C, Ferrari-Toninelli G, Prandelli C, Govoni S, Buoso E, Racchi M, Barcikowska M, Styczynska M, Szybinska A, Butterfield DA, Memo M, Uberti D

PLoS One. 2012

Highly Pathogenic Alzheimer's Disease Presenilin 1 P117R Mutation Causes a specific Increase in p53 and p21 Protein Levels and Cell Cycle Dysregulation in Human Lymphocytes

Bialopiotrowicz E, Szybinska A, Kuzniewska B, Buizza L, Uberti D, Kuznicki J, Wojda U

J Alzheimers Dis. 2012

Novel beta-catenin target genes identified in thalamic neurons encode modulators of neuronal excitability

Wisniewska MB, Nagalski A, Dabrowski M, Misztal K, Kuznicki J

BMC Genomics. 2012

2011

WNT protein-independent constitutive nuclear localization of beta-catenin protein and its low degradation rate in thalamic neurons.

Misztal K, Wisniewska MB, Ambrozkiewicz M, Nagalski A, Kuznicki J

J Biol Chem.. 2011

Store-operated calcium entry modulates neuronal network activity in a model of chronic epilepsy

Steinbeck JA, Henke N, Opatz J, Gruszczynska-Biegala J, Schneider L, Theiss S, Hamacher N, Steinfarz B, Golz S, Brüstle O, Kuznicki J, Methner A

Exp Neurol.. 2011

Cell cycle regulation distinguishes lymphocytes from sporadic and familial Alzheimer's disease patients

Bialopiotrowicz E, Kuzniewska B, Kachamakova-Trojanowska N, Barcikowska M, Kuznicki J, Wojda U

Neurobiol Aging. 2011

Calmyrin1 binds to SCG10 protein (stathmin2) to modulate neurite outgrowth

Sobczak A, Debowska K, Blazejczyk M, Kreutz MR, Kuznicki J, Wojda U

Biochim Biophys Acta. 2011

Differential Roles for STIM1 and STIM2 in Store-Operated Calcium Entry in Rat Neurons

Gruszczynska-Biegala J, Pomorski P, Wisniewska MB, Kuznicki J

PLoS One. 2011

2010

In vitro findings of alterations in intracellular calcium homeostasis in schizophrenia.

Bojarski L, Debowska K, Wojda U.

Prog Neuropsychopharmacol Biol Psychiatry. 2010

Increased CD44 gene expression in lymphocytes derived from Alzheimer disease patients.

Uberti D, Cenini G, Bonini SA, Barcikowska M, Styczynska M, Szybinska A, Memo M.

Neurodegener Dis. 2010

Resistance of the dopamine D4 receptor to agonist-induced internalization and degradation.

Spooren A, Rondou P, Debowska K, Lintermans B, Vermeulen L, Samyn B, Skieterska K, Debyser G, Devreese B, Vanhoenacker P, Wojda U, Haegeman G, Van Craenenbroeck K.

Cell Signal. 2010

Fizjologia i molekularny mechanizm działania glikokortykoidów.

Nagalski A, Kiersztan A.

Postepy Hig Med Dosw. 2010

LEF1/beta-catenin complex regulates transcription of the Cav3.1 calcium channel gene (Cacna1g) in thalamic neurons of the adult brain.

Wisniewska MB, Misztal K, Michowski W, Szczot M, Purta E, Lesniak W, Klejman ME, Dabrowski M, Filipkowski RK, Nagalski A, Mozrzymas JW, Kuznicki J.

J Neurosci. 2010

Morgana/CHP-1 is a novel chaperone able to protect cells from stress.

Michowski W, Ferretti R, Wisniewska MB, Ambrozkiewicz M, Beresewicz M, Fusella F, Skibinska-Kijek A, Zablocka B, Brancaccio M, Tarone G, Kuznicki J.

Biochim Biophys Acta. 2010

Mitochondrial genotype in vulvar carcinoma - cuckoo in the nest

Klemba A, Kowalewska M, Kukwa W, Tonska K, Szybinska A, Mossakowska M, Scinska A, Golik P, Koper K, Radziszewski J, Kukwa A, Czarnecka AM, Bartnik E

J Biomed Sci. 2010

An age-related decrease in factor V Leiden frequency among Polish subjects

Adler G, Parczewski M, Czerska E, Loniewska B, Kaczmarczyk M, Gumprecht J, Grzeszczak W, Szybinska A, Mossakowska M, Ciechanowicz A

J Appl Genet. 2010

Decreased expression and the Lys751Gln polymorphism of the XPD gene are associated with extreme longevity

Polosak J, Roszkowska-Gancarz M, Kurylowicz A, Owczarz M, Dobosz P, Mossakowska M, Szybinska A, Puzianowska-Kuznicka M

Biogerontology. 2010

Mitochondrial genotype and breast cancer predisposition

Czarnecka AM, Krawczyk T, Plak K, Klemba A, Zdrozny M, Arnold RS, Kofler B, Golik P, Szybinska A, Lubinski J, Mossakowska M, Bartnik E, Petros JA

Oncol Rep. 2010

2009

The ER and ageing II: calcium homeostasis.

Puzianowska-Kuznicka M, Kuznicki J.

Ageing Res Rev. 2009

Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2) in brain indicates its function in NMDA receptor mediated Ca2+ signaling.

Blazejczyk M, Sobczak A, Debowska K, Wisniewska MB, Kirilenko A, Pikula S, Jaworski J, Kuznicki J, Wojda U.

Arch Biochem Biophys. 2009

Presenilin-dependent expression of STIM proteins and dysregulation of capacitative Ca2+ entry in familial Alzheimer's disease.

Bojarski L, Pomorski P, Szybinska A, Drab M, Skibinska-Kijek A, Gruszczynska-Biegala J, Kuznicki J

Biochim Biophys Acta. 2009

Immunolocalization of STIM1 in the mouse brain.

Skibinska-Kijek A, Wisniewska MB, Gruszczynska-Biegala J, Methner A, Kuznicki J

Acta Neurobiol Exp (Wars). 2009

Expression of STIM1 in brain and puncta-like co-localization of STIM1 and ORAI1 upon depletion of Ca(2+) store in neurons.

Klejman ME, Gruszczynska-Biegala J, Skibinska-Kijek A, Wisniewska MB, Misztal K, Blazejczyk M, Bojarski L, Kuznicki J.

Neurochem Int. 2009

2008

The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1.

Sbroggiò M, Ferretti R, Percivalle E, Gutkowska M, Zylicz A, Michowski W, Kuznicki J, Accornero F, Pacchioni B, Lanfranchi G, Hamm J, Turco E, Silengo L, Tarone G, Brancaccio M.

FEBS Lett. 2008

Factfile: Calmyrin 1 (review)

Blazejczyk M, Wojda U

Calcium Binding Proteins. 2008

Involvement of S100A6 (calcyclin) and its binding partners in intracellular signaling pathways

Filipek A, Michowski W, Kuznicki J

Adv Enzyme Regul. 2008

Sgt1 has co-chaperone properties and is up-regulated by heat shock.

Zabka M, Leśniak W, Prus W, Kuźnicki J, Filipek A

Biochem Biophys Res Commun. 2008

Conformationally altered p53: a novel Alzheimer's disease marker?

Lanni C, Racchi M, Mazzini G, Ranzenigo A, Polotti R, Sinforiani E, Olivari L, Barcikowska M, Styczynska M, Kuznicki J, Szybinska A, Govoni S, Memo M, Uberti D

Mol Psychiatry. 2008

Calcium ions in neuronal degeneration

Wojda U, Salinska E, Kuznicki J

IUBMB Life. 2008

Calcium dysregulation in Alzheimer's disease

Bojarski L, Herms J, Kuznicki J

Neurochem Int. 2008

Polish Centenarians Programme. Multidisciplinary studies of successful ageing: aims, methods, and preliminary results

Mossakowska M, Barcikowska M, Broczek K, Grodzicki T, Klich-Raczka A, Kupisz-Urbanska M, Podsiadly-Marczykowska T, Sikora E, Szybinska A, Wieczorowska-Tobis K, Zyczkowska J, Kuznicki J

Exp Gerontol. 2008

Statins impair antitumor effects of rituximab by inducing conformational changes of CD20.

Winiarska M, Bil J, Wilczek E, Wilczynski GM, Lekka M, Engelberts PJ, Mackus WJ, Gorska E, Bojarski L, Stoklosa T, Nowis D, Kurzaj Z, Makowski M, Glodkowska E, Issat T, Mrowka P, Lasek W, Dabrowska-Iwanicka A, Basak GW, Wasik M, Warzocha K, Sinski M, Gaciong Z, Jakobisiak M, Parren PW, Golab J.

PLoS Med. 2008

Variation in NPC1, the gene encoding Niemann-Pick C1, a protein involved in intracellular cholesterol transport, is associated with Alzheimer disease and/or aging in the Polish population

Erickson RP, Larson-Thomé K, Weberg L, Szybinska A, Mossakowska M, Styczynska M, Barcikowska M, Kuznicki J

Neurosci Lett. 2008

2007

Association study of cholesterol-related genes in Alzheimer's disease

Wollmer MA, Sleegers K, Ingelsson M, Zekanowski C, Brouwers N, Maruszak A, Brunner F, Huynh KD, Kilander L, Brundin RM, Hedlund M, Giedraitis V, Glaser A, Engelborghs S, De Deyn PP, Kapaki E, Tsolaki M, Daniilidou M, Molyva D, Paraskevas GP, Thal DR, Barcikowska M, Kuznicki J, Lannfelt L, Van Broeckhoven C, Nitsch RM, Hock C, Papassotiropoulos A.

Neurogenetics. 2007

Hsp70 is a new target of Sgt1-an interaction modulated by S100A6

Spiechowicz M, Zylicz A, Bieganowski P, Kuznicki J,Filipek A

Biochem Biophys Res Commun. 2007

CacyBP/SIP interacts with tubulin in neuroblastoma NB2a cells and induces formation of globular tubulin assemblies

Schneider G, Nieznanski K, Kilanczyk E,Bieganowski P, Kuznicki J, Filipek A

Biochim Biophys Acta. 2007

Epigenetic control of the S100A6 (calcyclin) gene expression

Leśniak W, Słomnicki ŁP, Kuźnicki J

J Invest Dermatol. 2007

Biochemical properties of endogenous presenilin 1 and presenilin 2 in cultured human B-lymphocytes

Bojarski L, Lewandowicz A, Blazejczyk M, Sobczak A, Kuznicki J, Wojda U

Clin Chem Lab Med. 2007

2006

Ca2+-independent binding and cellular expression profiles question a significant role of calmyrin in transduction of Ca2+-signals to Alzheimer's disease-related presenilin 2 in forebrain

Blazejczyk M, Wojda U, Sobczak A, Spilker C, Bernstein HG, Gundelfinger ED, Kreutz MR, Kuznicki J

Biochim Biophys Acta. 2006

The relationship between adiponectin levels and metabolic status in centenarian, early elderly, young and obese women

Bik W, Baranowska-Bik A, Wolinska-Witort E, Martynska L, Chmielowska M, Szybinska A, Broczek K, Baranowska B

Neuro Endocrinol Lett. 2006

Neuroendocrine control of metabolic homeostasis in Polish centenarians

Baranowska B, Bik W, Baranowska-Bik A, Wolinska-Witort E, Szybinska A, Martynska L, Chmielowska M

J Physiol Pharmacol. 2006

Density of Sgt1-immunopositive neurons is decreased in the cerebral cortex of Alzheimer's disease brain.

Spiechowicz M, Bernstein HG, Dobrowolny H, Leśniak W, Mawrin C, Bogerts B, Kuźnicki J, Filipek A

Neurochem Int. 2006

Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter.

Leśniak W, Kuźnicki J

J Cell Biochem. 2006

Calretinin, an EF-hand calcium-binding proteins, binds zinc and Cooper

Groves P, Palczewska M, Kuznicki J

Calcium Binding Proteins. 2006

Two novel presenilin 1 gene mutations connected with frontotemporal dementia-like clinical phenotype: genetic and bioinformatic assessment.

Zekanowski C, Golan MP, Krzyśko KA, Lipczyńska-Łojkowska W, Filipek S, Kowalska A, Rossa G, Pepłońska B, Styczyńska M, Maruszak A, Religa D, Wender M, Kulczycki J, Barcikowska M, Kuźnicki J.

Exp Neurol.. 2006

2005

The -22c/t polymorphism in presenilin 1 gene is not connected with late-onset and early-onset familial Alzheimer's disease in Poland

Zekanowski C, Religa D, Safranow K, Maruszak A, Dziedziejko V, Styczyńska M, Gacia M, Golan M, Pepłońska B, Chlubek D, Kuźnicki J, Barcikowska M

J Neural Transm. 2005

Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form.

Sobczak A, Blazejczyk M, Piszczek G, Zhao G, Kuznicki J, Wojda U.

Acta Biochim Pol. 2005

Genetic alterations in accelerated ageing syndromes. Do they play a role in natural ageing?

Puzianowska-Kuznicka M, Kuznicki J.

Int J Biochem Cell Biol. 2005

Characterization of calretinin I-II as an EF-hand, Ca2+, H+-sensing domain

Palczewska M, Batta G, Groves P, Linse S, Kuznicki J

Protein Sci. 2005

Calcyclin (S100A6) expression is stimulated by agents evoking oxidative stress via the antioxidant response element.

Leśniak W, Szczepańska A, Kuźnicki J.

Biochim Biophys Acta. 2005

The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies

Bhattacharya S, Lee YT, Michowski W, Jastrzebska B, Filipek A, Kuznicki J, Chazin WJ

Biochemistry. 2005

The Alzheimer disease-related calcium-binding protein Calmyrin is present in human forebrain with an altered distribution in Alzheimer's as compared to normal ageing brains

Bernstein HG, Blazejczyk M, Rudka T, Gundelfinger ED, Dobrowolny H, Bogerts B, Kreutz MR, Kuznicki J, Wojda U

Neuropathol Appl Neurobiol. 2005

2004

Proliferation and apoptosis of human CD8(+)CD28(+) and CD8(+)CD28(-) lymphocytes during aging

Brzezińska A, Magalska A, Szybińska A, Sikora E

Exp Gerontol. 2004

Genetic aspects of Alzheimer's disease

Zekanowski C, Religa D, Graff C, Filipek S, Kuźnicki J

Acta Neurobiol Exp (Wars). 2004

The E318G substitution in PSEN1 gene is not connected with Alzheimer's disease in a large Polish cohort.

Zekanowski C, Pepłońska B, Styczyńska M, Religa D, Pfeffer A, Czyzewski K, Gabryelewicz T, Szybińska A, Kijanowska-Haładyna B, Kotapka-Minc S, Łuczywek E, Barczak A, Wasiak B, Chodakowska-Zebrowska M, Przekop I, Kuźnicki J, Barcikowska M

Neurosci Lett. 2004

Gene therapy of hematopoietic cells: development of strategies and vectors. (in Polish)

Wojda U.

Postepy Biol Kom. 2004

Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.

Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ.

J Biol Chem.. 2004

Calretinin gene promoter activity is differently regulated in neurons and cancer cells. Role of AP2-like cis element and zinc ions

Billing-Marczak K, Ziemińska E, Leśniak W, Łazarewicz JW, Kuźnicki J

Biochim Biophys Acta. 2004

2003

Molecular diagnostics of Alzheimer's disease (in Polish)

Zekanowski C

Medycyna ogólna. 2003

The sun, the genome, the internet… eugenics?

Zekanowski C

Gemellological Rev. 2003

Genetic diversity within the R408W phenylketonuria mutation lineages in Europe

Tighe O, Dunican D, O'Neill C, Bertorelle G, Beattie D, Graham C, Zschocke J, Cali F, Romano V, Hrabincova E, Kozak L, Nechyporenko M, Livshits L, Guldberg P, Jurkowska M, Zekanowski C, Perez B, Desviat LR, Ugarte M, Kucinskas V, Knappskog P, Treacy E, N

Hum Mutat. 2003

Alzheimer's disease: between diagnostic, economy and ethic (in Polish)

Zekanowski C, Przyluska-Fiszer A, Barcikowska M

Choroby otępienne. Teoria i praktyka. 2003

Mutation screening of the MAPT and STH genes in Polish patients with clinically diagnosed frontotemporal dementia

Zekanowski C, Pepłońska B, Styczyńska M, Gustaw K, Kuźnicki J, Barcikowska M

Dement Geriatr Cogn Disord. 2003

Mutations in presenilin 1, presenilin 2 and amyloid precursor protein genes in patients with early-onset Alzheimer's disease in Poland

Zekanowski C, Styczyńska M, Pepłońska B, Gabryelewicz T, Religa D, Ilkowski J, Kijanowska-Haładyna B, Kotapka-Minc S, Mikkelsen S, Pfeffer A, Barczak A, Łuczywek E, Wasiak B, Chodakowska-Zebrowska M, Gustaw K, Łaczkowski J, Sobów T, Kuźnicki J, Barcikowsk

Exp Neurol. 2003

Strong association between Saitohin gene polymorphism and tau haplotype in the Polish population

Pepłońska B, Zekanowski C, Religa D, Czyzewski K, Styczyńska M, Pfeffer A, Gabryelewicz T, Gołebiowski M, Luczywek E, Wasiak B, Barczak A, Chodakowska M, Barcikowska M, Kuźnicki J

Neurosci Lett. 2003

Calretinin and calbindin D28k have different domain organizations

Palczewska M, Groves P, Batta G, Heise B, Kuźnicki J

Protein Sci. 2003

Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins

Nowotny M, Spiechowicz M, Jastrzebska B, Filipek A, Kitagawa K, Kuznicki J

J Biol Chem. 2003

2002

AP2-like cis element is required for calretinin gene promoter activity in cells of neuronal phenotype differentiated from multipotent human cell line DEV.

Billing-Marczak K, Buzanska L, Winsky L, Nowotny M, Rudka T, Isaacs K, Belin MF, Kuznicki J.

Biochim Biophys Acta. 2002

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family

Filipek A, Jastrzebska B, Nowotny M, Kuznicki J

J Biol Chem. 2002

Genetic aspects of Alzheimer’s disease

Zekanowski C

Medycyna Ogólna. 2002

Novel PKU treatment methods

Zekanowski C, Gizewska M, Nowacka M, Cabalska B

Medycyna Wieku Rozwojowego. 2002

Atypical PKU - treatment effectiveness

Cabalska B, Nowacka M, Nowaczewska I, Zekanowski C, Zorska K

Medycyna Wieku Rozwojowego VI. 2002

Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells

Filipek A, Jastrzebska B, Nowotny M, Kwiatkowska K, Hetman M, Surmacz L, Wyroba E,Kuznicki J

J Biol Chem. 2002

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family

Filipek A, Jastrzebska B, Nowotny M, Kuznicki J

J Biol Chem. 2002

AP2-like cis element is required for calretinin gene promoter activity in cells of neuronal phenotype differentiated from multipotent human cell line DEV

Billing-Marczak K, Buzanska L, Winsky L, Nowotny M, Rudka T, Isaacs K, Belin MF, Kuznicki J

Biochim Biophys Acta. 2002

2001

Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93)

Palczewska M, Groves P, Ambrus A, Kaleta A, Kövér KE, Batta G, Kuźnicki J.

Eur J Biochem.. 2001

2000

Regulation of cell specific expression of calcyclin (S100A6) in nerve cells and other tissues.

Leśniak W, Swart GW, Bloemers HP, Kuźnicki J.

Acta Neurobiol Exp (Wars). . 2000

Upstream stimulatory factor is involved in the regulation of the human calcyclin (S100A6) gene.

Leśniak W, Jezierska A, Kuźnicki J.

Biochim Biophys Acta.. 2000

Calcyclin (S100A6) binding protein (CacyBP) is highly expressed in brain neurons.

Jastrzebska B, Filipek A, Nowicka D, Kaczmarek L, Kuznicki J.

J Histochem Cytochem.. 2000

Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein.

Nowotny M, Bhattacharya S, Filipek A, Krezel AM, Chazin W, Kuznicki J.

J Biol Chem.. 2000

Polish Centenarians Program “PolStu99” – search for longevity promoting factors (in Polish)

Mossakowska M, Puzianowska-Kuznicka M, Barcikowska M, Chiron-Jouan S, Czyzewski K, Derejczyk J, Franceschi C, Gabryelewicz T, Galus K, Grodzicki T, Gross R, Klich-Raczka A, Luczywek E, Passeri G, Pfeffer A, Pruszynski J, Radziszewska E, Sikora E, Sosnowski M, Styczynska M, Wasiak B, Wieczorowska-Tobis K, Zyczkowska J, Kuznicki J

Gerontologia. 2000

Cloning - the crossroads of biology and culture

Zekanowski C

Gemellological Review. 2000