DESCRIPTION OF CURRENT RESEARCH

Mammalian/mechanistic target of rapamycin (mTOR) is a serine-threonine kinase that is involved in almost every aspect of mammalian cell function. It forms two protein complexes, initially identified as regulating translation (mTOR complex 1 [mTORC1]) or influencing the actin cytoskeleton (mTORC2). My postdoctoral work showed that the regulation of mTOR-dependent translation contributes to dendritogenesis (Jaworski et al., J Neurosci, 2005). This was subsequently confirmed by our recent work in which we identified the GluA2 subunit of glutamate receptors as a protein that is both translated in an mTORC1-dependent manner and vital for dendritogenesis (Koscielny et al., Mol Neurobiol, 2018). However, the list of cellular processes that involve both mTORCs has expanded, and new ways of regulating mTORC activity, novel mTOR partners, and mTOR effectors have been discovered. Nonetheless, their contribution to the neuronal functions of mTOR and neuropathology is still poorly understood. Therefore, since the inception of our laboratory, we have sought to identify mTOR partners and regulated proteins that are involved in neuronal development and characterize mTOR dysfunction in neuropathology.

To reach our scientific objectives, we have been primarily using a well-established, relatively simple, and robust model of the dendritogenesis of neurons that are cultured in vitro. Using this approach, we performed both proof-of-principle experiments and unbiased screens that clearly demonstrated mTOR functions during neuronal development beyond the canonical control of translation (e.g., regulation of the cytoskeleton and transcription). These experiments also extended our general knowledge of molecular mechanisms downstream of mTOR and new mechanisms that underlie dendritogenesis (Swiech et al., J Neurosci, 2011; Urbanska et al., J Biol Chem, 2012 & Sci Rep, 2017; Malik et al., J Biol Chem, 2013).

Progress in meeting our research goals allowed us to merge some objectives and hone our main focus toward identification of the cellular compartment-specific regulation and functions of mTOR in developing neurons, with a particular focus on intracellular trafficking events, which were at the center of our research efforts during the last 6 years (Main Research Objective 1). Notably, both the role of mTOR in intracellular trafficking control and the role of membrane trafficking in neuronal development and disease are still understudied topics.

Therefore, focusing on these areas (e.g., the interplay between mTORCs and molecular motors, such as the dynein-dynactin complex and kinesins, and small GTPases of the Rab family and their regulators) creates an opportunity to successfully proceed with our research in otherwise extremely crowded fields of the molecular biology of mTOR and mTOR-related disorders.

An important part of our work during the last 6 years has been to develop and characterize new approaches to study mTOR functions in vivo beyond dendritogenesis (i.e., in utero brain electroporation in rodents and transgenic zebrafish) and in clinically relevant material (e.g., patient samples, primary cultures, induced pluripotent stem cells, and organoids). These modern techniques, together with newly identified mTOR-controlled molecular processes, are critically important for our second main objective, namely understanding the molecular pathology of mTORopathies (Main Research Objective 2), which are diseases that are related to mTOR dysregulation (e.g., tuberous sclerosis complex (TSC) and epilepsy).

By studying mTOR in the context of the control of dendritic arbor morphology, we identified a significant gap in the literature about this phenomenon. Dendritic arbor morphology is unique for different types of neurons and reflects their precise adjustment to functions they perform within particular neuronal networks. Although dendrites must remain intact for more than 80% of a neuron’s lifespan, little is known about the molecular mechanisms that underlie this phenomenon. To date, very few proteins have been identified to be essential for the stability of mature dendritic arbors. Disturbances in dendritic arbor stability in the mature brain are related to prolonged stress and mood disorders (e.g., depression). At later stages of brain aging, when cognitive decline develops, dendrites may also deteriorate. Intriguingly, recent studies reported changes in mTOR signaling in mood disorders and aging. Thus, our new Main Research Objective 3 seeks to understand the molecular mechanisms of dendrite stability and their disruption in mood disorders and the aging brain.

Our major success in 2019 falls within Main Research Objective 2, which focuses on mTORrelated disorders. One such disease is TSC. It is a multiorgan disease that is caused by mutations of the TSC1 and TSC2 genes, the products of which form a complex (TSC complex) that inhibits mTORC1 (Switon et al., IUBMB Life, 2016). The most common symptoms of TSC are epilepsy, autism, the formation of benign tumors in the brain, and socalled TSC-associated neuropsychiatric disorders (TANDs). We used zebrafish with the mutated Tsc2 gene to study causal links between elementary neurodevelopment and epilepsy/TANDs. We found that the lack of Tsc2 in zebrafish resulted in heterotopias (Fig. 1A) and hyperactivation of the mTorC1 pathway in pallial regions, which are homologous to the mammalian cortex (Kedra et al., Proc Natl Acad Sci USA, 2020). We observed commissural thinning (Fig. 1B) that was responsible for brain dysconnectivity, recapitulating TSC pathology in humans (Kedra et al., Proc Natl Acad Sci USA, 2020). The mutants exhibited epileptogenesis that resulted in nonmotor seizures and an increase in anxiety-like behavior (i.e., one symptom of TANDs; Kedra et al., 2020; Fig. 1C,D), which were rescued by reducing tyrosine receptor kinase B (TrkB) signaling. TrkB inhibition also rescued brain dysconnectivity. These data provide a mechanistic link between brain anatomy and human TANDs and identify TrkB as a possible lead target in the search for TAND-specific drugs.

fig.1

fig.1. Zebrafish model of Tuberous Sclerosis Complex (tsc2vu242/vu242) can be used to study several disease symptoms. (A) Visualization of cell nuclei using DAPI shows that in TSC fish brain several cells are mislocalized causing disruption of white matter. AC - anterior commissure (B) ptf1a:GFP-positive neurons in the posterior tuberculum extend their axons dorsally and cross the brain midline through the posterior commissure (left panel). In the TSC fish these axonal tracts are disorganized and do not cross the midline as compact bundle (right panel). (C) TSC fish display absence seizure-like phenotype that can be “cured” with prolonged ethosuximide treatment used typically in clinic. (D) In open field test TSC fishes avoid center of the dish suggesting increased anxiety. For more details, see Kedra et al., Proc Natl Acad Sci USA, 2020. Photo: Justyna Zmorzyńska, PhD and Magdalena Kędra, MSc.

 

 jjaworski

Jacek Jaworski, PhD, Professor

Correspondence address:
Laboratory of Molecular and Cellular Neurobiology
International Institute of Molecular and Cell Biology
4 Ks. Trojdena Street, 02-109 Warsaw, Poland
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
tel: +48 (22) 597 0755; fax: +48 (22) 597 0715

DEGREES

2014 - Professor of Biological Sciences, nomination by the President of the Republic of Poland
2010 - DSc Habil in Molecular Biology, Warsaw University, Poland
2001 - PhD in Molecular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1996 - MSc in Biology, Department of Genetics, Warsaw University, Poland

PROFESSIONAL EXPERIENCE

2018-present - Deputy Director for Science, International Institute of Molecular and Cell Biology in Warsaw, Poland
2010 - 2013 - Deputy Director, International Institute of Molecular and Cell Biology in Warsaw, Poland
2005-present - Professor, Head of Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology in Warsaw, Poland

RESEARCH TRAINING

2016 - Research visit (3 weeks) with Prof. William Harris, Cambridge University, Cambridge, UK
2011 - Research visit (2 weeks) with Dr. Carlo Sala, CNR Institute of Neuroscience and Instituto Neurologico Carlo Besta, Milan, Italy
2006 - Research visit (1 month) with Dr. C.C. Hoogenraad, Erasmus Medical Center, Rotterdam, Holland
2002-2005 - Postdoctoral Associate with Prof. Morgan Sheng, Picower Center for Learning and Memory, Massachusetts Institute of Technology and Howard Hughes Medical Institute, Cambridge, MA, USA
2000 - Research training with Dr. J. Guzowski, ARL Division of Neural Systems, Memory and Aging, University of Arizona, Tucson, USA
1997-2001 - Research training (7 months) with Prof. J. Mallet, Laboratoire de Genetique Moleculaire de la Neurotransmission et des Processus Neurodegeneratifs (LGN), UMR 9923 CNRS, Paris, France
1996-2002 - PhD student (until 2001) and Postdoctoral Associate (until May 2002) with Prof. L. Kaczmarek, Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
1995-1996 - Master’s degree, Prof. P. Węgleński, Department of Genetics, Warsaw University, Poland

FELLOWSHIPS AND AWARDS

2018 - TEAM, Foundation for Polish Science
2014 - Master Award, Foundation for Polish Science
2011 - Prime Minister Award for habilitation thesis
2009 - 2nd Division (Biological Sciences) of Polish Academy of Sciences Award for series of publications on MMP9 (together with teams of Prof. Kaczmarek and Dr. Wilczynski)
2005 - Konorski Award for best publication of 2004 in the field of neuroscience (Kowalczyk et al., JCB, 2004, 167:209-213), Polish Neuroscience Society and Polish Academy of Sciences
2002 - Prime Minister Award for PhD thesis
2001 - Foundation for Polish Science National Scholarship for Young Investigators (1 year scholarship)
2000 - EMBO Short Term Fellowship
1999 - Polish Network for Cell and Molecular Biology UNESCO/PAN Scholarship
1997 - Bourse de Stage du Gouvernement Francaise (French Government Scholarship)

MEMBERSHIP IN SCIENTIFIC SOCIETIES, ORGANIZATIONS, AND PANELS

2019 - Member, Scientific Advisory Board of the Institute of Pharmacology, Polish Academy of Sciences
2017 - Vice President, Polish Neuroscience Society (term 2017-2019)
2015 - Corresponding Member, Warsaw Scientific Society
2015 - Member, Scientific Advisory Board of the Nencki Institute of Experimental Biology, Polish Academy of Sciences
2011 - Member, Neurobiology Committee, Polish Academy of Sciences (terms 2011-2014; 2015-2018; 2019-2020)

DOCTORATES DEFENDED UNDER LAB LEADER’S SUPERVISION

Ł. Świech, A. Malik, M. Perycz, M. Urbańska, A. Skałecka, J. Lipka, A. Urbańska, M. Firkowska, K. Kisielewska, A. Kościelny.

team2019

 

Lab Leader:

  •  Jacek Jaworski, PhD, Professor

Senior Researchers:

  • Ewa Liszewska, PhD

  • Matylda Macias, PhD (part-time)

  • Małgorzata Urbańska, PhD

  • Justyna Zmorzyńska, PhD

  • Agnieszka Brzozowska, PhD

Postdoctoral Researchers:

  • Andrii Kopach, PhD

  • Aleksandra Tempes, PhD

  • Tomasz Dulski, PhD

  • Roberto Pagano, PhD

Research Assistant:

  •  Bartosz Frycz, PhD

Research Technicians:

  • Marek Sarnacki, MSc

PhD Students:

  • Magdalena Kędra, MSc

  • Magdalena Mlostek, MSc

  • Karolina Bogusz, MSc

  • Oliver Tkaczyk, MSc

  • Jan Węsławski, MSc

  • Juan Zeng, MSc

  • Olga Doszyń, MSc

  • Shiwani Kumari, MSc

Lab Technician:

  • Alina Zielińska, BSc (part-time)

Laboratory Support Specialist:

  • Angelika Jocek, MSc

 

2022

SRF depletion in early life contributes to social interaction deficits in the adulthood.

Roszkowska M, Krysiak A, Majchrowicz L, Nader K, Beroun A, Michaluk P, Pekala M, Jaworski J, Kondrakiewicz L, Puścian A, Knapska E, Kaczmarek L, Kalita K.

Cell Mol Life Sci.. 2022

SRF depletion in early life contributes to social interaction deficits in the adulthood.

Roszkowska M, Krysiak A, Majchrowicz L, Nader K, Beroun A, Michaluk P, Pekala M, Jaworski J, Kondrakiewicz L, Puścian A, Knapska E, Kaczmarek L, Kalita K.

Cell Mol Life Sci.. 2022

miRNAs and isomiRs: Serum-Based Biomarkers for the Development of Intellectual Disability and Autism Spectrum Disorder in Tuberous Sclerosis Complex.

Scheper M, Romagnolo A, Besharat ZM, Iyer AM, Moavero R, Hertzberg C, Weschke B, Riney K, Feucht M, Scholl T, Petrak B, Maulisova A, Nabbout R, Jansen AC, Jansen FE, Lagae L, Urbanska M, Ferretti E, Tempes A, Blazejczyk M, Jaworski J, Kwiatkowski DJ, Jozwiak S, Kotulska K, Sadowski K, Borkowska J, Curatolo P, Mills JD, Aronica E, Epistop Consortium Members.

Biomedicines. 2022

Generation of three human iPSC lines from patients with Huntington’s disease with different CAG lengths and human control iPSC line from a healthy donor.

Latoszek E, Piechota M, Liszewska E, Hansíkova ́H, Klempír J,
Mühlback A, Landwehrmeyer GB, Kuznicki J, Czeredys M.

Stem Cell Res.. 2022

2021

Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle.

Liszewska E, Majchrowicz L, Krogulec E, Kotulska K, Kaczmarek L, Kalita K, Dobrzyń A, Jaworski J.

Stem Cell Res.. 2021

mTOR controls endoplasmic reticulum-Golgi apparatus trafficking of VSVg in specific cell types.

Koscielny A, Liszewska E, Machnicka K, Wezyk M, Kotulska K, Jaworski J.

Cell Mol Biol Lett.. 2021

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Prentzell MT et al. (Jaworski J, Kedra M, Kopach A, Macias M, Zmorzynska J.)

Cell. 2021

Prevention of Epilepsy in Infants with Tuberous Sclerosis Complex in the EPISTOP Trial.

Kotulska K, Kwiatkowski DJ, Curatolo P, Weschke B, Riney K, Jansen F, Feucht M, Krsek P, Nabbout R, Jansen AC, Wojdan K, Sijko K, Głowacka-Walas J, Borkowska J, Sadowski K, Domańska-Pakieła D, Moavero R, Hertzberg C, Hulshof H, Scholl T, Benova B, Aronica E, de Ridder J, Lagae L, Jóźwiak S; EPISTOP Investigators (Jaworski J, Błażejczyk M, Tempes A, Urbańska M.)

Ann Neurol. 2021

2020

TrkB hyperactivity contributes to brain dysconnectivity, epileptogenesis, and anxiety in zebrafish model of Tuberous Sclerosis Complex.

Kedra M, Banasiak K, Kisielewska K, Wolinska-Niziol L, Jaworski J, Zmorzynska J.

Proc Natl Acad Sci U S A. . 2020

Role of dynein-dynactin complex, kinesins, motor adaptors and their phosphorylation in dendritogenesis.

Tempes A, Weslawski J, Brzozowska A, Jaworski J.

J Neurochem.. 2020

TSC2 pathogenic variants are predictive of severe clinical manifestations in TSC infants: results of the EPISTOP study.

Ogórek B, Hamieh L, Hulshof HM, Lasseter K, Klonowska K, Kuijf H, Moavero R, Hertzberg C, Weschke B, Riney K, Feucht M, Scholl T, Krsek P, Nabbout R, Jansen AC, Benova B, Aronica E, Lagae L, Curatolo P, Borkowska J, Sadowski K, Domańska-Pakieła D, Janson S, Kozlowski P, Urbanska M, Jaworski J, Jozwiak S, Jansen FE, Kotulska K; EPISTOP Consortium members, Kwiatkowski DJ.

Genet Med. . 2020

2019

ESCRT Proteins Control the Dendritic Morphology of Developing and Mature Hippocampal Neurons.

Firkowska M, Macias M, Jaworski J.

Mol Neurobiol.. 2019

GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation.

Urbanska M⁠⁠,⁠ Kazmierska-Grebowska⁠ P, Kowalczyk⁠ T., Caban B⁠, Nader⁠ K, Pijet⁠ B, Kalita ⁠K, Gozdz⁠ A, Devijver⁠e H, Lechat⁠e B, Jaworski T⁠, Grajkowska⁠ W, Sadowski⁠ K, Jozwiak⁠ S⁠,⁠ Kotulska⁠ K, Konopacki⁠ J, Van Leuven⁠ F, van Vliet⁠h E⁠, Aronica⁠ E⁠,⁠ Jaworski⁠ J.

EBioMedicine. 2019

Pathological mTOR mutations impact cortical development.

Tarkowski B, Kuchcinska K, Blazejczyk M, Jaworski J.

Hum Mol Genet.. 2019

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.

Rojek KO, Krzemień J, Doleżyczek H, Boguszewski PM, Kaczmarek L, Konopka W, Rylski M, Jaworski J, Holmgren L, Prószyński TJ.

PLoS Biol.. 2019

DNA polymerase ι is acetylated in response to SN2 alkylating agents.

McIntyre J, Sobolewska A, Fedorowicz M, McLenigan MP, Macias M, Woodgate R, Sledziewska-Gojska E.

Sci Rep.. 2019

Cytosolic translational responses differ under conditions of severe short-term and long-term mitochondrial stress.

Samluk L, Urbanska M, Kisielewska K, Mohanraj K, Kim MJ, Machnicka K, Liszewska E, Jaworski J, Chacinska A.

Mol Biol Cell.. 2019

2018

Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58.

Hareza A, Bakun M, Świderska B, Dudkiewicz M, Koscielny A, Bajur A, Jaworski J, Dadlez M, Pawłowski K.

PeerJ.. 2018

c-Fos and neuronal plasticity: the aftermath of Kaczmarek's theory.

Jaworski J, Kalita K, Knapska E.

Acta Neurobiol Exp (Wars). . 2018

Neural Stem Cell Dysfunction in Human Brain Disorders.

Liszewska E, Jaworski J.

Results Probl Cell Differ.. 2018

The level of microRNA 21 is upregulated by rapamycin in serum of tuberous sclerosis complex patients and subependymal giant cell astrocytoma (SEGA)-derived cell cultures.

Kuzniewska B, Sadowski K, Urbanska K, Urbanska M, Kotulska K, Liszewska E, Grajkowska W, Jóźwiak S, Dziembowska M.

Folia Neuropathol. . 2018

Adaptor Complex 2 Controls Dendrite Morphology via mTOR-Dependent Expression of GluA2

Koscielny A, Malik AR, Liszewska E, Zmorzynska J, Tempes A, Tarkowski B, Jaworski J

Mol Neurobiol. 2018

Blocking c-Fos Expression Reveals the Role of Auditory Cortex Plasticity in Sound Frequency Discrimination Learning

de Hoz L, Gierej D, Lioudyno V, Jaworski J, Blazejczyk M, Cruces-Solís H, Beroun A, Lebitko T, Nikolaev T, Knapska E, Nelken I, Kaczmarek L

Cereb Cortex. 2018

GSK3β Controls mTOR and Prosurvival Signaling in Neurons.

Urbanska M, Gozdz A, Macias M, Cymerman IA, Liszewska E, Kondratiuk I, Devijver H, Lechat B, Van Leuven F, Jaworski J.

Mol Neurobiol.. 2018

2017

GSK-3β and MMP-9 Cooperate in the Control of Dendritic Spine Morphology

Kondratiuk I, Łęski S, Urbańska M, Biecek P, Devijver H, Lechat B, Van Leuven F, Kaczmarek L, Jaworski T

Mol Neurobiol. 2017

Kainic Acid Induces mTORC1-Dependent Expression of Elmo1 in Hippocampal Neurons

Blazejczyk M, Macias M, Korostynski M, Firkowska M, Piechota M, Skalecka A, Tempes A, Koscielny A, Urbanska M, Przewlocki R, Jaworski J

Mol Neurobiol. 2017

Molecular neurobiology of mTOR

Switon K, Kotulska K, Janusz-Kaminska A, Zmorzynska J, Jaworski J

Neuroscience. 2017

Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration

Kononenko NL, Claßen GA, Kuijpers M, Puchkov D, Maritzen T, Tempes A, Malik AR, Skalecka A, Bera S, Jaworski J, Haucke V

Nat Commun. 2017

ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons

Urbanska AS, Janusz-Kaminska A, Switon K, Hawthorne AL, Perycz M, Urbanska M, Bassell GJ, Jaworski J

Sci Rep. 2017

GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation

Gozdz A, Nikolaienko O, Urbanska M, Cymerman IA, Sitkiewicz E, Blazejczyk M, Dadlez M, Bramham CR, Jaworski J

Front Mol Neurosci. 2017

2016

Dextran Nanoparticle Synthesis and Properties

Wasiak I, Kulikowska A, Janczewska M, Michalak M, Cymerman IA, Nagalski A, Kallinger P, Szymanski WW, Ciach T

PLoS One. 2016

Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites

Lipka J, Kapitein LC, Jaworski J, Hoogenraad CC

EMBO J. 2016

Kinesin-Binding Protein Controls Microtubule Dynamics and Cargo Trafficking by Regulating Kinesin Motor Activity

Kevenaar JT, Bianchi S, van Spronsen M, Olieric N, Lipka J,Frias CP, Mikhaylova M, Harterink M, Keijzer N, Wulf PS, Hilbert M, Kapitein LC, de Graaff E, Ahkmanova A, Steinmetz MO, Hoogenraad CC

Curr Biol. 2016

Expression of microRNAs miR21, miR146a, and miR155 in tuberous sclerosis complex cortical tubers and their regulation in human astrocytes and SEGA-derived cell cultures

van Scheppingen J, Iyer AM, Prabowo AS, Mühlebner A, Anink JJ, Scholl T, Feucht M, Jansen FE, Spliet WG, Krsek P, Zamecnik J, Buccoliero AM, Giordano F, Genitori L, Kotulska K, Jozwiak S, Jaworski J, Liszewska E, van Vliet EA, Aronica E

Glia. 2016

mTOR kinase is needed for the development and stabilization of dendritic arbors in newly born olfactory bulb neurons

Skałecka A, Liszewska E, Bilinski R, Gkogkas C, Khoutorsky A, Malik AR, Sonenberg N, Jaworski J

Dev Neurobiol. 2016

Tuberous sclerosis complex: From molecular biology to novel therapeutic approaches

Switon K, Kotulska K, Janusz-Kaminska A, Zmorzynska J, Jaworski J

IUBMB Life. 2016

miR-132 Regulates Dendritic Spine Structure by Direct Targeting of Matrix Metalloproteinase 9 mRNA

Jasińska M, Miłek J, Cymerman IA, Łęski S, Kaczmarek L, Dziembowska M

Mol Neurobiol.. 2016

Okiem ryby - nowe spojrzenie na rozwój sieci neuronalnych i choroby neurorozwojowe.

Kędra M, Jaworski J, Zmorzyńska J.

Wszechświat . 2016

2015

Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3βMistargeted mitochondrial proteins activate a proteostatic response in the cytosol

Cymerman IA, Gozdz A, Urbanska M, Milek J, Dziembowska M, Jaworski J

PLoS One. 2015

Matricellular proteins of the Cyr61/CTGF/NOV (CCN) family and the nervous system

Malik AR, Liszewska E, Jaworski J

Front Cell Neurosci. 2015

Tuberous sclerosis complex neuropathology requires glutamate-cysteine ligase

Malik AR, Liszewska E, Skalecka A, Urbanska M, Iyer AM, Swiech LJ, Perycz M, Parobczak K, Pietruszka P, Zarebska MM, Macias M, Kotulska K, Borkowska J, Grajkowska W, Tyburczy ME, Jozwiak S, Kwiatkowski DJ, Aronica E, Jaworski J

Acta Neuropathol Commun. 2015

Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture

Esteves da Silva M, Adrian M, Schätzle P, Lipka J, Watanabe T, Cho S, Futai K, Wierenga CJ, Kapitein LC, Hoogenraad CC

Cell Rep. 2015

Molecular mechanisms of induced pluripotency.

Kulcenty K, Wróblewska J, Mazurek S, Liszewska E, Jaworski J.

Contemp Oncol (Pozn). 2015

2014

CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi

Skupien A, Konopka A, Trzaskoma P, Labus J, Gorlewicz A, Swiech L, Babraj M, Dolezyczek H, Figiel I, Ponimaskin E, Wlodarczyk J, Jaworski J, Wilczynski GM, Dzwonek J

J Cell Sci. 2014

The GRIP1/14-3-3 Pathway Coordinates Cargo Trafficking and Dendrite Development

Geiger JC, Lipka J, Segura I, Hoyer S, Schlager MA, Wulf PS, Weinges S, Demmers J, Hoogenraad CC, Acker-Palmer A

Dev Cell. 2014

2013

Beyond control of protein translation: What we have learned about the non-canonical regulation and function of mammalian target of rapamycin (mTOR)

Malik AR, Urbanska M, Macias M, Skalecka A, Jaworski J

Biochim Biophys Acta. 2013

CYR61, a matricellular protein, is needed for dendritic arborization of hippocampal neurons

Malik AR, Urbanska M, Gozdz A, Swiech LJ, Nagalski A, Perycz M, Blazejczyk M, Jaworski J

J Biol Chem. 2013

TRAK/Milton Motor-Adaptor Proteins Steer Mitochondrial Trafficking to Axons and Dendrites

van Spronsen M, Mikhaylova M, Lipka J, Schlager MA, van den Heuvel DJ, Kuijpers M, Wulf PS, Keijzer N, Demmers J, Kapitein LC, Jaarsma D, Gerritsen HC, Akhmanova A, Hoogenraad CC

Neuron. 2013

Brain-derived neurotrophic factor induces matrix metalloproteinase-9 expression in neurons via serum response factor/c-Fos pathway

Kuzniewska B, Rejmak E, Malik AR, Jaworski J, Kaczmarek L, Kalita K

Mol Cell Biol. 2013

Myosin-V Opposes Microtubule-Based Cargo Transport and Drives Directional Motility on Cortical Actin

Kapitein LC, van Bergeijk P, Lipka J, Keijzer N, Wulf PS, Katrukha EA, Akhmanova A, Hoogenraad CC

Curr Biol. 2013

Spatiotemporal Characterization of mTOR Kinase Activity Following Kainic Acid Induced Status Epilepticus and Analysis of Rat Brain Response to Chronic Rapamycin Treatment

Macias M, Blazejczyk M, Kazmierska P, Caban B, Skalecka A, Tarkowski B, Rodo A, Konopacki J, Jaworski J

PLoS One. 2013

Adhesion, but not a specific cadherin code, is indispensable for ES cell and induced pluripotency

Bedzhov I, Alotaibi H, Basilicata MF, Ahlborn K,Liszewska E, Brabletz T, Stemmler MP

Stem Cell Res. 2013

The fragile x mental retardation protein regulates matrix metalloproteinase 9 mRNA at synapses

Janusz A, Milek J, Perycz M, Pacini L, Bagni C, Kaczmarek L, Dziembowska M

J Neurosci. 2013

Mutations in cytoplasmic dynein and its regulators cause malformations of cortical development and neurodegenerative diseases

Lipka J, Kuijpers M,Jaworski J, Hoogenraad CC

Biochem Soc Trans. 2013

Why do we need induced pluripotent stem cells in neurobiology?

Liszewska E, Jaworski J

Postepy Biochem. 2013

Uchwytny kształt pamięci

Jaworski J

Biologia w szkole. 2013

2012

Developmental plasticity of the dendritic compartment: focus on the cytoskeleton

Urbanska M, Swiech L, Jaworski J

Adv Exp Med Biol. 2012

Mammalian target of rapamycin complex 1 (MTORC1) and 2 MTORC2) control the dendritic arbor morphology of hippocampal neurons

Urbanska M, Gozdz A, Swiech LJ, Jaworski J

JBC. 2012

Functional anatomy of neural circuits regulating fear and extinction

Knapska E, Macias M, Mikosz M, Nowak A, Owczarek D, Wawrzyniak M, Pieprzyk M, Cymerman IA, Werka T, Sheng M, Maren S, Jaworski J, Kaczmarek L

Proc. Natl. Acad. Sci.. 2012

2011

2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family

Werner M, Purta E, Kaminska KH, Cymerman IA, Campbell DA, Mittra B, Zamudio JR, Sturm NR, Jaworski J, Bujnicki JM

Nucleic Acids Res. 2011

Distinct molecular regulation of glycogen synthase kinase-3alpha isozyme controlled by its N-terminal region: functional role in calcium/calpain signaling

Azoulay-Alfaguter I, Yaffe Y, Licht-Murava A, Urbanska M, Jaworski J, Pietrokovski S, Hirschberg K, Eldar-Finkelman H

J Biol Chem. 2011

CLIP-170 and IQGAP1 cooperatively regulate dendrite morphology

Swiech L, Blazejczyk M, Urbanska M, Pietruszka P, Dortland BR, Malik AR, Wulf PS, Hoogenraad CC, Jaworski J

J Neurosci. 2011

Zipcode binding protein 1 regulates the development of dendritic arbors in hippocampal neurons

Perycz M, Urbanska AS, Krawczyk PS, Parobczak K, Jaworski J

J Neurosci. 2011

NMDA Receptor Activation Suppresses Microtubule Growth and Spine Entry

Kapitein LC, Yau KW, Gouveia SM, van der Zwan WA, Wulf PS, Keijzer N, Demmers J, Jaworski J, Akhmanova A, Hoogenraad CC

J Neurosci. 2011

2010

Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.

Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K

J Neurochem. 2010

Production and characterization of recombinant protein preparations of Endonuclease G-homologs from yeast, C. elegans and humans.

Kieper J, Lauber C, Gimadutdinow O, Urbańska A, Cymerman I, Ghosh M, Szczesny B, Meiss G

Protein Expr Purif. 2010

The dissection of transcriptional modules regulated by various drugs of abuse in the mouse striatum.

Piechota M, Korostynski M, Solecki W, Gieryk A, Slezak M, Bilecki W, Ziolkowska B, Kostrzewa E, Cymerman I, Swiech L, Jaworski J, Przewlocki R

Genome Biol. 2010

2009

Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2) in brain indicates its function in NMDA receptor mediated Ca2+ signaling.

Blazejczyk M, Sobczak A, Debowska K, Wisniewska MB, Kirilenko A, Pikula S, Jaworski J, Kuznicki J, Wojda U.

Arch Biochem Biophys. 2009

Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity.

Jaworski J, Kapitein LC, Gouveia SM, Dortland BR, Wulf PS, Grigoriev I, Camera P, Spangler SA, Di Stefano P, Demmers J, Krugers H, Defilippi P, Akhmanova A, Hoogenraad CC

Neuron. 2009

2008

Inducible cAMP early repressor (ICER)-evoked delayed neuronal death in the organotypic hippocampal culture

Mioduszewska B, Jaworski J, Szklarczyk AW, Klejman A, Kaczmarek L

J Neurosci Res. 2008

Molecular basis of dendritic arborization

Urbanska M, Blazejczyk M, Jaworski J

Acta Neurobiol Exp (Wars). 2008

Injury induced dendritic plasticity in the mature central nervous system.

Macias M

Acta Neurobiol Exp (Wars). 2008

Role of mTOR in physiology and pathology of the nervous system

Swiech L, Perycz M, Malik A, Jaworski J

Biochim Biophys Acta. 2008

Microtubule plus-end tracking proteins in differentiated mammalian cells.

Jaworski J, Hoogenraad CC, Akhmanova A

Int J Biochem Cell Biol. 2008

Yin Yang 1 is a critical repressor of matrix metalloproteinase-9 expression in brain neurons

Rylski M, Amborska R, Zybura K, Mioduszewska B, Michaluk P, Jaworski J, Kaczmarek L

J Biol Chem. 2008

Organelle-specific zinc detection using zinpyr-labeled fusion proteins in live cells

Tomat E, Nolan EM, Jaworski J, Lippard SJ

J Am Chem Soc. 2008

2007

TIMP-1 abolishes MMP-9-dependent long-lasting long-term potentiation in the prefrontal cortex.

Okulski P, Jay TM, Jaworski J, Duniec K, Dzwonek J, Konopacki FA, Wilczynski GM, Sánchez-Capelo A, Mallet J, Kaczmarek L

Biol Psychiatry. 2007

Beta-dystroglycan as a target for MMP-9, in response to enhanced neuronal activity.

Michaluk P, Kolodziej L, Mioduszewska B, Wilczynski GM, Dzwonek J, Jaworski J, Gorecki DC, Ottersen OP, Kaczmarek L

J Biol Chem. 2007

ARF6 in the nervous system.

Jaworski J

Eur J Cell Biol. 2007

2006

Increased estrogen receptor beta expression correlates with decreased spine formation in the rat hippocampus

Szymczak S, Kalita K, Jaworski J, Mioduszewska B, Savonenko A, Markowska A, Merchenthaler I, Kaczmarek L

Hippocampus. 2006

Zinspy sensors with enhanced dynamic range for imaging neuronal cell zinc uptake and mobilization.

Nolan EM, Ryu JW, Jaworski J, Feazell RP, Sheng M, Lippard SJ

J Am Chem Soc. 2006

The growing role of mTOR in neuronal development and plasticity

Jaworski J, Sheng M

Mol Neurobiol. 2006