Our research utilizes the zebrafish model organism to study how gene expression is regulated in the developing embryo, examining its links to congenital malformations in humans. We specifically focus on heart development and diseases, employing classical genetics alongside bulk and single-cell genomics techniques.
Research Summary
Intricate embryonic patterning is achieved through highly precise regulatory mechanisms that ensure controlled gene expression in the correct time and space. Our research aims to decipher the mechanism by which gene expression is regulated by transcription factors (TFs) and the epigenetic landscape. By exploring these mechanisms, we aim to illuminate how disruptions contribute to human congenital malformations. While key genetic factors that regulate the development and function of the heart are known, understanding their regulation, interactions, and coordination with epigenetic factors at different phases of heart development remains a gap. Our research focuses on cardiomyocytes and cardiac pacemaker cells.
Scientific Impact
We have generated transcriptomics and epigenomics resources of the developing zebrafish heart, covering the cardiomyocytes as well as rare cell types such as pacemakers. Our most recent work established a single cell transcriptome atlas of the developing zebrafish heart, revealing new cell types and their molecular profile. Additionally, our investigations have also revealed putative novel regulatory elements implicated in generating the cardiovascular cell diversity.
Future Goals
We aim to generate zebrafish models of human genetic diseases which could be used for in-depth studies to elucidate disease mechanisms or screening for potential therapies. Ultimately, we hope that our research could contribute to a better understanding of the complex molecular pathways underlying human congenital diseases.
Collaborations
We collaborate with labs within the IIMCB as well as externally. We also collaborate internationally with leading labs in the genomics and clinical genetics field.
Comment
“Eventually, we believe that our research in zebrafish must benefit humans. Therefore, we are always striving to select candidates which possess clinical relevance or potential for therapy.” - Cecilia Lanny Winata, PhD, DSc Habil
