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  • Laboratory of Neurodegeneration: Kuznicki Laboratory

Laboratory of Neurodegeneration: Kuznicki Laboratory

Jacek Kuźnicki, PhD, Professor 

Correspondence address:
Laboratory of Neurodegeneration
International Institute of Molecular and Cell Biology
4 Ks. Trojdena Street, 02-109 Warsaw, Poland
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Degrees:
1993    Professor, awarded by the President of the Republic of Poland
1987    DSc. Habil., Nencki Institute of Experimental Biology PAS, Warsaw, Poland
1980    PhD in biochemistry, Nencki Institute of Experimental Biology PAS, Warsaw, Poland
1976    MSc in biochemistry, Faculty of Biology, University of Warsaw, Poland

Postdoctoral Training:
Jul 2018        Special Volunteer in Harold Burgess Lab, National Institute of Child Health and Human Development, Bethesda
Jul 2015        Visiting Professor, partnership Laboratory (Prof. William Harris) within the FishMed project, University of Cambridge  
Jul 2014        Visiting Professor, partnership Laboratory (Prof. B. E. Snaar-Jagalska) within the FishMed project, Leiden University 
1992-1995     Visiting Scientist in David Jacobowitz’s Lab, National Institute of Mental Health, Lab of Clinical Science, Bethesda
1981-1984     Visiting Fellow (postdoc in Ed D. Korn laboratory), National Institute of Heart, Lung and Blood, Laboratory of Cell Biology, Bethesda

Professional Employment:
2017-present   Deputy Chair of the Council of Provosts, 2nd Division: Biological and Agricultural Sciences, Polish Academy of Sciences
2001-2018        Director of the International Institute of Molecular and Cell Biology in Warsaw
2001-present    Head of the Laboratory of Neurodegeneration at IIMCB
2000-2001        Director, Centre of Excellence for Studies on Mechanisms of Neurodegeneration Phare Sci-Tech II at the Nencki Inst.
1999-2001        Acting Director, IIMCB; Organizer and Director of Centenarian Program
1996-2002        Head of the Calcium Binding Proteins Lab., professor 2002-2014, Nencki Inst.
1992-1995        Visiting Professor, NIMH, Bethesda, MD, USA
1991-1992        Deputy Scientific Director, Nencki Institute
1986-1992        Associate Professor, Head of the Calcium Binding Proteins Lab., Nencki Inst.
1984-1985        Research Associate, Nencki Institute
1981-1984        Visiting Fellow, NIH, Bethesda, MD, USA
1980-1981        Post-doctoral Fellow, Nencki Institute
1976-1980        PhD Student, Nencki Institute of Experimental Biology PAN, Warsaw

Membership in Scientific Societies, Organizations and Panels:
•    Panel for activity evaluation of the Committee for Spatial Economy and Regional Planning, Polish Academy of Sciences, member 2017
•    Advisory panel for the PAS President scholarships for outstanding achievements for PhD students in the PAS units, member 2017
•    Consultative Council, II Faculty of Medicine with English Language Division at the Medical University of Lublin, member since 2017
•    Program Committee of the 44th FEBS Congress From molecules to living systems in 2019, member since 2017
•    Steering Committee of the InnoNeuroPharm Sectorial Program, National Centre for Research and Development Centre, member 2016-2018
•    Advisory panel for developing rules and regulation on membership in the Polish Academy of Sciences, member since 2016
•    International Advisory Board of the Malopolska Centre of Biotechnology (MCB), Jagiellonian University in Cracow, member since 2016
•    Forecast Committee Polska 2000 Plus of the Presidium of the Polish Academy of Sciences, member since 2016
•    Centre of Pre-clinical Research and Technology (CePT), rotating president: 4.03.2016-31.12.2016
•    Program Board of the Polish Academy of Sciences Scientific Station in Rome, member 2015-2018
•    Ministry of Economy in Poland - Working Group for National Smart Specializations, member since 2014
•    European Cooperation in Science and Technology (COST), Management Committee Member in Action BM1406, acronym: IONCHAN-IMMUNRESPON, since 2014
•    EU Joint Programme - Neurodegenerative Disease Research (JPND), reviewer 2014
•    Consortium Biocentrum Ochota, rotating president 1.07.2010-31.12.2010, 1.07.2013-31.12.2013 and 1.07.2016-31.12.2016, member since 2008
•    Federation of European Neuroscience Societies, member since 2013
•    Expert in panels of Life Sciences at the National Science Centre, 2012-2014, 2016-2017, 2018
•    Scientific Council of the Nencki Institute of Experimental Biology, member until 2014, and the Mossakowski Medical Research Centre PAS, member 2002-2014
•    M. Nencki’s Foundation to Support the Biological Sciences, board member since 2012
•    Scientific Policy Committee in the Ministry of Science and Higher Education, rotating president 07.2012-12.2012, member 2011-2014
•    Research and Education Center State Key Laboratory of Molecular and Cellular Biology (SKL) in Ukraine, International Expert Council since 2011
•    BIO-IMAGINE Steering Committee, FP7 at the Nencki Institute, member 2011-2014
•    Society for Neuroscience, member since 2010
•    Polish Alzheimer’s Society, member of the Scientific Council since 2009
•    European Calcium Society (ECS), Board member since 2008
•    Head of the Scientific & Organizing Committees of the 11th ECS Conference, Warsaw 2010
•    Prime Minister Award Committee, member 2007-2009
•    Advisory Group of the FP7 for Health, EC, Brussels, member 2006-2011
•    Corresponding member of the Polish Academy of Sciences, since 2004
•    American Society for Biochemistry & Molecular Biology (ASBMB), 2003-2017
•    Centre for Innovative Bioscience Education, head of the Program Board since 2002
•    Biochemical Society (Royal Society) in England, member 1995-2010
•    Polish Neuroscience Society, member since 1991
•    Society for the Advancement of Science, member 1991-2009
•    Committees of the Polish Academy of Sciences: Committee on Cell Biology, member 1990-1992, 1995-2002, 2007-2015; Committee on Neurobiology, member 1999-2002, 2005-2015; Biotechnology Committee, vice-president 1996-1998, 2000-2002, member 1990-2002; Committee of Biochemistry and Biophysics, member 1999-2002
•    Journals: Pharmacological Reports, Advisory Board member since 2017; Biochemical Journal, editorial advisor 1995-2010; Advances in Biochemistry, member of the Editorial Board 1989-1992; Acta Biochimica Polonica, member of the Editorial Board since 1997; Advances in Cell Biology, member of the Editorial Board 2000
•    Polish Biochemical Society, member since 1977, General Secretary 1989-1991

Honors, Prizes and Awards:
• 2018 Commemorative medal for the 100th Anniversary of the Nencki Institute for outstanding contribution to the development of this Institute, supporting its activities, shaping the image and building a mutual success
• 2013 Crystal Brussels Prize for outstanding achievements in 7th Framework Programme of the European Union for Research and Development
• 2013 Award of the 2nd Division of Biological and Agricultural Sciences of the Polish Academy of Sciences for Dr. Marta B. Wisniewska, Dr. Katarzyna Misztal, Andrzej Nagalski - PhD student and Prof. Jacek Kuznicki for a series of research papers on β-catenin
• 2011 Konorski Award for the best Polish research work in neurobiology awarded by the Polish Neuroscience Society and Committee on Neurobiology of the PAS, Warsaw
• 2008 Officer's Cross of the Order of Polonia Restituta awarded by the President of Poland
• 2004-2007 Professorial Subsidy Program Award from Foundation for Polish Science
• 2003 Prime Minister Award for the scientific achievements, Warsaw
• 2001 Award from the Division of Biological Sciences of the PAS for the work on CaBP
• 1998 Knight's Cross of the Order of Polonia Restituta awarded by the President of  Poland
• 1987 Polish Anatomical Society Award for the article in “Advances in Cell Biology”
• 1986 Skarzynski Award from the Polish Biochemical Society for the best review article in “Advances in Biochemistry”, Warsaw
• 1977 Mozolowski Award, Polish Biochem. Society for outstanding Polish young biochemist
• 1977 Parnas Award from Polish Biochem. Society for the best paper in biochemical research
• 1976 MSc, Magna cum laude, University of Warsaw

Research focus

We are interested in the molecular mechanisms that are involved in neurodegeneration, with a special emphasis on the role of Ca2+ homeostasis and signaling. These processes are being studied at the genomic, proteomic, and cellular levels using zebrafish, rats, and mice as model organisms. The projects are focused on proteins that are involved in store-operated calcium entry (SOCE), the involvement of potassium channels in the brain ventricular system, and the in vivo analysis of calcium homeostasis in neurons using zebrafish models.

Role of STIM proteins in store-operated calcium entry in neurons

We previously showed that STIM1 is involved in thapsigargin-induced SOCE, whereas STIM2 is mostly active after the EGTA-driven depletion of extracellular calcium (Gruszczynska-Biegala et al., PLoS One, 2011; Gruszczynska-Biegala and Kuznicki, J Neurochem, 2013). We searched for new partners of STIM proteins other than ORAI channels and found that endogenous STIMs physically associate with GluA subunits of AMPA receptors. The SOCE inhibitors reduced AMPA-induced Ca2+ influx, and AMPA antagonists decreased neuronal SOCE. These results suggested the involvement of AMPA receptors in neuronal SOCE (Gruszczynska-Biegala et al., Front Cell Neurosci, 2016).

Dysregulation of calcium homeostasis in neurodegenerative diseases

The vast majority of available animal models of Alzheimer’s disease (AD) are based on the β-amyloid/tau hypothesis. These mice overexpress one or more mutated proteins that are known to be responsible for early-onset familial Alzheimer’s disease (FAD). Models of FAD, representing less than 5% of all human cases, appear to have little value for understanding the mechanisms of sporadic AD (SAD; for review, see Wojda and Kuznicki, J Alzheimers Dis, 2013). Thus, new models need to be developed that incorporate some features of SAD. We tested the hypothesis that brain dysfunction during ageing is induced by changes in Ca2+ homeostasis, which may predispose the brain to SAD pathologies. Transgenic mice that overexpressed key SOCE proteins (STIM1, STIM2, and Orai1) specifically in brain neurons under the Thy1 promoter were generated. Characterization of the STIM1 line (Majewski et al., BBA Mol Cell Res, 2017) and Orai1 line (Majewski et al., Society for Neuroscience, 2017, abstract no. 477.06/P6) has been reported. The double transgenic line that expressed STIM2 and Orai1 proteins in neurons was generated, and its phenotype is being characterized.

FAD mutations in presenilins were shown to alter both endoplasmic reticulum (ER) calcium signaling and SOCE, but the role of amyloid precursor protein (APP) and APP FAD mutants in intracellular calcium homeostasis is controversial. Our data indicate that APP and APP FAD mutants are not directly involved in SOCE (Wegierski et al., Biochem Biophys Res Commun, 2016). Instead, we found that APP knockdown resulted in an elevation of the resting levels of ER Ca2+, reduced Ca2+ leakage, and delayed the translocation of STIM1 to Orai1 upon ER Ca2+ store depletion. Our data suggest a regulatory role for APP in ER Ca2+ (Gazda et al., Sci Rep, 2017).

To explore calcium homeostasis during the early stages of SAD and mild cognitive impairment (MCI), we investigated SOCE and inositol triphosphate receptor 3-mediated calcium release into the cytoplasm in unmodified B-lymphocytes from MCI subjects and SAD patients and compared them with non-demented subjects. We observed perturbed calcium homeostasis in peripheral cells from MCI and SAD patients, supporting the hypothesis that SAD is a systemic disease, and MCI is a risk factor for AD (Jaworska et al., BBA Mol Cell Res, 2013; for review, see Majewski and Kuznicki, BBA Mol Cell Res, 2015).

Using qRT-PCR, we compared microRNA (miRNA) profiles in blood plasma from MCI-AD patients (whose diagnoses were confirmed by cerebrospinal fluid [CSF] biomarkers) with AD patients and non-demented, age-matched controls. We adhered to standardized blood and CSF assays that are recommended by the JPND BIOMARKAPD consortium, and we employed commercially available Exiqonq RT-PCR-assays. Six miRNAs (three not yet reported in the context of AD and three reported in AD blood) were selected as the most promising biomarker candidates that can differentiate early AD from controls with the highest fold changes (Nagaraj et al., Oncotarget, 2017; patent pending, PCT/IB2016/052440).

We previously showed that a mutation of huntingtin (HTT) in YAC128 mice (i.e., a model of Huntington’s disease) resulted in the higher expression of some components of the calcium signalosome, including huntingtin-associated protein 1 (HAP1) and calcyclin (S100A6) binding protein (CacyBP/SIP; Czeredys et al., Front Mol Neurosci, 2013). We detected greater activity of SOC channels in medium spiny neurons (MSNs) from YAC128 mice and found that some tetrahydrocarbazoles restored the disturbances in Ca2+ homeostasis and stabilized SOCE in YAC128 MSN cultures (Czeredys et al., Biochem Biophys Res Commun, 2017). The overexpression of HAP1 protein isoform A in MSNs from YAC128 mice was found to enhance the activity of SOC channels after the activation of IP3R1.

In collaboration with Prof. Oliver Bandmann (University of Sheffield), we used a pink1 mutant (pink-/-) zebrafish line to study possible alterations of Ca2+ homeostasis (Flinn et al., Ann Neurol, 2013). A loss-of-function mutation of pink1 causes early-onset Parkinson’s disease in humans. We found that both genetic and pharmacological inhibition of the mitochondrial Ca2+ uniporter rescued dopaminergic neurons in pink1-/- zebrafish by reversing mitochondrial respiratory chain function (Soman et al., Eur J Neurosci, 2016). This work is being continued to establish the role of calcium homeostasis in mitochondria in pink1-/- fish.

Brain ventricle project

Formation of the brain ventricular system (BVS) occurs during the early neural development of vertebrates (Korzh, Cell Mol Life Sci, 2018). Deficiency of the BVS has been linked to numerous neurodegenerative diseases. The BVS depends on many factors, including the crucial role of ependyma (i.e., cells that line the BVS cavity and circumventricular organs, including the choroid plexus; Garcia-Lecea et al., Front Neuroanat, 2017). We previously found that ependyma expresses subunits of the voltage-gated potassium channels Kcnb1 (Kv2.1) and Kcng4 (Kv6.4), which antagonize each other. The deficiency of Kcnb1 in zebrafish causes microcephaly, and the gain-of-function of Kcnb1 causes hydrocephalus. Kcng4 acts in a reverse manner (Shen et al., Sci Rep, 2016). To study the role of K+ channels in the development of ependyma, we are currently designing and developing transgenic knockin zebrafish that express fluorescent proteins in ependyma under the control of Kcng4b regulatory elements and mutants of Kcng4b using the CRISPR-Cas9 mutagenesis system.

Lab Leader:
JACEK KUŹNICKI, PhD, Professor

Vice Head:
ŁUKASZ MAJEWSKI, PhD

Senior Staff Scientists:
VLADIMIR KORZH, PhD, Professor
TOMASZ WĘGIERSKI, PhD (part-time)

Senior Scientists:
MAGDA CZEREDYS, PhD
JOANNA GRUSZCZYŃSKA-BIEGAŁA, PhD
MAŁGORZATA WIWEGER, PhD

Postdoctoral Fellows:
GASANOV EVGENY, PhD
OKSANA PALCHEVSKA, PhD
SERGEY PALCHEVSKY, PhD (jointly with M. Nowotny’s Lab)

PhD Students:
JUSTYNA JĘDRYCHOWSKA, MSc
RISHIKESH KUMAR GUPTA, M Tech.
FILIP MACIĄG, MSc
IGA WASILEWSKA, MSc

Technical Assistant:
MICHAŁ BAZAŁA, MSc

Technician:
MONIKA MATUSZCZYK

Current affiliations of former employees:
• Urszula Wojda PhD, Professor, Head of Laboratory of Preclinical Testing of Higher Standard, Nencki Institute of Experimental Biology, Warsaw
• Marta Barbara Wiśniewska PhD, Professor, Head of Laboratory of Molecular Neurobiology, Center of New Technologies, Warsaw
• Emilia Białopiotrowicz PhD, postdoctoral research fellow, Laboratory of Experimental Hematology, Institute of Hematology and Transfusion Medicine, Warsaw
• Łukasz Bojarski PhD, Centralized Monitoring Therapy Area Lead at AstraZeneca
• Magdalena Błażejczyk PhD, postdoctoral research fellow, Laboratory of Molecular and Cellular Neurobiology, IIMCB
• Wojciech Michowski PhD, postdoctoral research fellow, Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA
• Katarzyna Misztal PhD, Senior Staff Scientists, IIMCB
• Andrzej Nagalski PhD, Laboratory of Molecular Neurobiology, Center of New Technologies, Warsaw
• Katarzyna Potrzebowska (Dębowska) PhD, Research Engineer, Leukocyte Migration Group, Immunology Section, Department of Experimental Medical Sciences, Lund University, Lund, Sweden
• Adam Sobczak PhD, postdoctoral research fellow, Institute of Biochemistry and Biophysics Polish Academy of Sciences, and Bio&Technology Innovations Platform of BioCentrum Ochota (BioTech-IP)
• Łukasz Szewczyk PhD, Laboratory of Molecular Neurobiology, Center of New Technologies, Warsaw
• Mateusz Ambrożkiewicz, MSc/PhD Program in Neurosciences, Georg-August University and European Neurosciences Institute at Gottingen/ International Max Planck Research School
• Bożena Kuźniewska, PhD student in the laboratory of Prof. Leszek Kaczmarek, Laboratory of Neurobiology, Nencki Institute of Experimental Biology, Warsaw, present researcher of Laboratory of Molecular Basis of Synaptic Plasticity, Center of New Technologies, Warsaw
• Aleksandra Mężydło (Kurek) Msc, PhD student in the Institute of Clinical Neuroimmunology Ludwig-Maximilians-Universität München
• Danuta Korona, student at Deutsches Krebsforschungszentrum (DKFZ), Studying Cancer Biology at University of Heidelberg, Germany
• Maria Śladowska Msc, PhD student in the Laboratory of Mitochondrial Biogenesis, IIMCB

2018

Wegierski T, Kuznicki J.

Neuronal calcium signaling via store-operated channels in health and disease.

Rydzanicz M, Wachowska M, Cook EC, Lisowski P, Kuźniewska B, Szymańska K, Diecke S, Prigione A, Szczałuba K, Szybińska A, Koppolu A, Murcia Pienkowski V, Kosińska J, Wiweger M, Kostrzewa G, Brzozowska M, Domańska-Pakieła D, Jurkiewicz E, Stawiński P, Gromadka A, Zielenkiewicz P, Demkow U, Dziembowska M, Kuźnicki J, Creamer TP, Płoski R.

Novel calcineurin A (PPP3CA) variant associated with epilepsy, constitutive enzyme activation and downregulation of protein expression.

Czeredys M, Vigont VA, Boeva VA, Mikoshiba K, Kaznacheyeva EV, Kuznicki J.

Huntingtin-Associated Protein 1A Regulates Store-Operated Calcium Entry in Medium Spiny Neurons From Transgenic YAC128 Mice, a Model of Huntington’s Disease.

Hadar A, Milanesi E, Walczak M, Puzianowska-Kuźnicka M, Kuźnicki J, Squassina A, Niola P, Chillotti C, Attems J, Gozes I, Gurwitz D.

SIRT1, miR-132 and miR-212 link human longevity to Alzheimer's Disease.

2017

Szewczyk LM, Brozko N, Nagalski A, Rӧckle I, Werneburg S, Hildebrandt H, Wisniewska MB, Kuznicki J free access

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons

Czeredys M, Maciag F, Methner A, Kuznicki J

Tetrahydrocarbazoles decrease elevated SOCE in medium spiny neurons from transgenic YAC128 mice, a model of Huntington's disease

Misztal K, Brozko N, Nagalski A, Szewczyk LM, Królak M, Brzozowska K, Kuznicki J, Wisniewska MB

TCF7L2 mediates the cellular and behavioral response to chronic lithium treatment in animal models

Soman S, Keatinge M, Moein M, DaCosta M, Mortiboys H, Skupin A, Sugunan S, Bazala M, Kuznicki J, Bandmann O

Inhibition of the mitochondrial calcium uniporter (MCU) rescues dopaminergic neurons in pink1-/- zebrafish

Brendel M, Kleinberger G, Probst F, Jaworska A, Overhoff F, Blume T, Albert NL, Carlsen J, Lindner S, Gildehaus FJ, Ozmen L, Suárez-Calvet M, Bartenstein P, Baumann K, Ewers M, Herms J, Haass C, Rominger A

Increase of TREM2 during Aging of an Alzheimer’s Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis

Nagaraj S, Laskowska-Kaszub K, Dębski KJ, Wojsiat J, Dąbrowski M, Gabryelewicz T, Kuźnicki J, Wojda U

Profile of 6 microRNA in blood plasma distinguish early stage Alzheimer's disease patients from non-demented subjects

Tan SY, Teh C, Ang CY, Li M, Li P, Korzh V, Zhao Y

Responsive mesoporous silica nanoparticles for sensing of hydrogen peroxide and simultaneous treatment toward heart failure

Koh CH, Wu J, Chung YY, Liu Z, Zhang RR, Chong K, Korzh V, Ting S, Oh S, Shim W, Tian HY, Wei H

Identification of a Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides

Shen H, Shin EM, Lee S, Mathavan S, Koh H, Osato M, Choi H, Tergaonkar V, Korzh V

Ikk2 regulates cytokinesis during vertebrate development

Korzh V

Development of brain ventricular system

Szybinska A, Lesniak W

P53 Dysfunction in Neurodegenerative Diseases – The Cause or Effect of Pathological Changes?

Gazda K, Kuznicki J, Wegierski T.

Knockdown of amyloid precursor protein increases calcium levels in the endoplasmic reticulum.

Majewski Ł, Maciąg F, Boguszewski PM, Wasilewska I, Wiera G, Wójtowicz T, Mozrzymas J, Kuznicki J.

Overexpression of STIM1 in neurons in mouse brain improves contextual learning and impairs long-term depression.

Brendel M, Focke C, Blume T, Peters F, Deussing M, Probst F, Jaworska A, Overhoff F, Albert N, Lindner S, von Ungern-Sternberg B, Bartenstein P, Haass C, Kleinberger G, Herms J, Rominger A.

Time Courses of Cortical Glucose Metabolism and Microglial Activity Across the Life Span of Wild-Type Mice: A PET Study.

Mazurek T, Kobylecka M, Zielenkiewicz M, Kurek A, Kochman J, Filipiak KJ, Mazurek K, Huczek Z, Królicki L, Opolski G.

PET/CT evaluation of 18F-FDG uptake in pericoronary adipose tissue in patients with stable coronary artery disease: Independent predictor of atherosclerotic lesions' formation?

Gazda K, Bazała M, Węgierski T.

Mikroskopowe obrazowanie jonów wapnia za pomocą genetycznie kodowanych sond (Microscopic imaging of calcium ions with genetically encoded calcium indicators).

Woźniak A, Grześkowiak BF, Babayevska N, Zalewski T, Drobna M, Woźniak-Budych M, Wiweger M, Słomski R, Jurga S.

ZnO@Gd2O3 core/shell nanoparticles for biomedical applications: Physicochemical, in vitro and in vivo characterization.

García-Lecea M, Gasanov E, Jedrychowska J, Kondrychyn I, Teh C, You MS, Korzh V.

Development of Circumventricular Organs in the Mirror of Zebrafish Enhancer-Trap Transgenics.

2016

Nagalski A, Puelles L, Dabrowski M, Wegierski T, Kuznicki J, Wisniewska MB

Molecular anatomy of the thalamic complex and the underlying transcription factors

Wegierski T, Gazda K, Kuznicki J

Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein

Gruszczynska-Biegala J, Sladowska M, Kuznicki J

AMPA Receptors Are Involved in Store-Operated Calcium Entry and Interact with STIM Proteins in Rat Primary Cortical Neurons

Goś D, Szymańska E, Białek-Wyrzykowska U, Wiweger M, Kuźnicki J.

Be Healthy as a Fish Educational Program at the International Institute of Molecular and Cell Biology in Warsaw, Poland.

2015

Majewski L, Kuznicki J

SOCE in neurons: Signaling or just refilling?

Brendel M, Jaworska A, Herms J, Trambauer J, Rötzer C, Gildehaus FJ, Carlsen J, Cumming P, Bylund J, Luebbers T, Bartenstein P, Steiner H, Haass C, Baumann K, Rominger A

Amyloid-PET predicts inhibition of de novo plaque formation upon chronic γ-secretase modulator treatment

Vassetzky Y, Gout I, Kuznicki J.

Research reforms: Ukrainian science needs elixir of youth.

2014

Honarnejad K, Daschner A, Gehring AP, Szybinska A, Giese A, Kuznicki J, Bracher F, Herms J

Identification of tetrahydrocarbazoles as novel multifactorial drug candidates for treatment of Alzheimer's disease

Mossakowska M , Broczek K, Wieczorowska-Tobis K, Klich-Raczka A, Jonas M, Pawlik-Pachucka E, Safranow K, Kuznicki J , Puzianowska-Kuznicka M

Cognitive Performance and Functional Status Are the Major Factors Predicting Survival of Centenarians in Poland

Mills F, Bartlett TE, Dissing-Olesen L, Wisniewska MB, Kuznicki J, Macvicar BA, Wang YT, Bamji SX

Cognitive flexibility and long-term depression (LTD) are impaired following β-catenin stabilization in vivo

2013

Nagalski A, Irimia M, Szewczyk L, Ferran JL, Misztal K, Kuznicki J, Wisniewska MB

Postnatal isoform switch and protein localization of LEF1 and TCF7L2 transcription factors in cortical, thalamic, and mesencephalic regions of the adult mouse brain

Esteras N, Alquézar C, Bermejo-Pareja F, Bialopiotrowicz E, Wojda U, Martín-Requero A

Downregulation of extracellular signal-regulated kinase 1/2 activity by calmodulin KII modulates p21Cip1 levels and survival of immortalized lymphocytes from Alzheimer's disease patients

Kapustian LL, Vigontina OA, Rozhko OT, Ryabenko DV, Michowski W, Lesniak W, Filipek A, Kroupskaya IV, Sidorik LL

Hsp90 and its co-chaperone, Sgt1, as autoantigens in dilated cardiomyopathy

Malik AR, Urbanska M, Gozdz A, Swiech LJ, Nagalski A, Perycz M, Blazejczyk M, Jaworski J

CYR61, a matricellular protein, is needed for dendritic arborization of hippocampal neurons

Wisniewska MB

Physiological Role of β-Catenin/TCF Signaling in Neurons of the Adult Brain

Golanska E, Sieruta M, Gresner SM, Pfeffer A, Chodakowska-Zebrowska M, Sobow TM, Klich I, Mossakowska M, Szybinska A, Barcikowska M, Liberski PP

APBB2 genetic polymorphisms are associated with severe cognitive impairment in centenarians

Jaworska A, Dzbek J, Styczynska M, Kuznicki J

Analysis of calcium homeostasis in fresh lymphocytes from patients with sporadic Alzheimer's disease or mild cognitive impairment

Golanska E, Sieruta M, Corder E, Gresner SM, Pfeffer A, Chodakowska-Zebrowska M, Sobow TM, Klich I, Mossakowska M, Szybinska A, Barcikowska M, Liberski PP

The prion protein M129V polymorphism: Longevity and cognitive impairment among Polish centenarians

Wojda U, Kuznicki J

Alzheimer's disease modeling: ups, downs, and perspectives for human induced pluripotent stem cells

Gruszczynska-Biegala J, Kuznicki J

Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons

Honarnejad K, Kirsch AK, Daschner A, Szybinska A, Kuznicki J, Herms J

FRET-Based Calcium Imaging: A Tool for High-Throughput/Content Phenotypic Drug Screening in Alzheimer Disease

Czeredys M, Gruszczynska-Biegala J, Schacht T, Methner A, Kuznicki J

Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington’s disease

Honarnejad K, Daschner A, Giese A, Zall A, Schmidt B, Szybinska A, Kuznicki J, Herms J

Development and Implementation of a High-Throughput Compound Screening Assay for Targeting Disrupted ER Calcium Homeostasis in Alzheimer's Disease

Wegierski T

Mikroskopowe metody rejestracji wewnątrzkomórkowych odpowiedzi wapniowych

2012

Hokkanen S, Feldmann HM, Ding H, Jung CK, Bojarski L, Renner-Müller I, Schüller U, Kretzschmar H, Wolf E, Herms J

Lack of Pur-alpha alters postnatal brain development and causes megalencephaly

Buizza L, Cenini G, Lanni C, Ferrari-Toninelli G, Prandelli C, Govoni S, Buoso E, Racchi M, Barcikowska M, Styczynska M, Szybinska A, Butterfield DA, Memo M, Uberti D

Conformational altered p53 as an early marker of oxidative stress in Alzheimer's disease

Bialopiotrowicz E, Szybinska A, Kuzniewska B, Buizza L, Uberti D, Kuznicki J, Wojda U

Highly Pathogenic Alzheimer's Disease Presenilin 1 P117R Mutation Causes a specific Increase in p53 and p21 Protein Levels and Cell Cycle Dysregulation in Human Lymphocytes

Wisniewska MB, Nagalski A, Dabrowski M, Misztal K, Kuznicki J

Novel beta-catenin target genes identified in thalamic neurons encode modulators of neuronal excitability

2011

Misztal K, Wisniewska MB, Ambrozkiewicz M, Nagalski A, Kuznicki J

WNT protein-independent constitutive nuclear localization of beta-catenin protein and its low degradation rate in thalamic neurons.

Steinbeck JA, Henke N, Opatz J, Gruszczynska-Biegala J, Schneider L, Theiss S, Hamacher N, Steinfarz B, Golz S, Brüstle O, Kuznicki J, Methner A

Store-operated calcium entry modulates neuronal network activity in a model of chronic epilepsy

Bialopiotrowicz E, Kuzniewska B, Kachamakova-Trojanowska N, Barcikowska M, Kuznicki J, Wojda U

Cell cycle regulation distinguishes lymphocytes from sporadic and familial Alzheimer's disease patients

Sobczak A, Debowska K, Blazejczyk M, Kreutz MR, Kuznicki J, Wojda U

Calmyrin1 binds to SCG10 protein (stathmin2) to modulate neurite outgrowth

Gruszczynska-Biegala J, Pomorski P, Wisniewska MB, Kuznicki J

Differential Roles for STIM1 and STIM2 in Store-Operated Calcium Entry in Rat Neurons

2010

Bojarski L, Debowska K, Wojda U.

In vitro findings of alterations in intracellular calcium homeostasis in schizophrenia.

Uberti D, Cenini G, Bonini SA, Barcikowska M, Styczynska M, Szybinska A, Memo M.

Increased CD44 gene expression in lymphocytes derived from Alzheimer disease patients.

Spooren A, Rondou P, Debowska K, Lintermans B, Vermeulen L, Samyn B, Skieterska K, Debyser G, Devreese B, Vanhoenacker P, Wojda U, Haegeman G, Van Craenenbroeck K.

Resistance of the dopamine D4 receptor to agonist-induced internalization and degradation.

Nagalski A, Kiersztan A.

Fizjologia i molekularny mechanizm działania glikokortykoidów.

Wisniewska MB, Misztal K, Michowski W, Szczot M, Purta E, Lesniak W, Klejman ME, Dabrowski M, Filipkowski RK, Nagalski A, Mozrzymas JW, Kuznicki J.

LEF1/beta-catenin complex regulates transcription of the Cav3.1 calcium channel gene (Cacna1g) in thalamic neurons of the adult brain.

Michowski W, Ferretti R, Wisniewska MB, Ambrozkiewicz M, Beresewicz M, Fusella F, Skibinska-Kijek A, Zablocka B, Brancaccio M, Tarone G, Kuznicki J.

Morgana/CHP-1 is a novel chaperone able to protect cells from stress.

Klemba A, Kowalewska M, Kukwa W, Tonska K, Szybinska A, Mossakowska M, Scinska A, Golik P, Koper K, Radziszewski J, Kukwa A, Czarnecka AM, Bartnik E

Mitochondrial genotype in vulvar carcinoma - cuckoo in the nest

Adler G, Parczewski M, Czerska E, Loniewska B, Kaczmarczyk M, Gumprecht J, Grzeszczak W, Szybinska A, Mossakowska M, Ciechanowicz A

An age-related decrease in factor V Leiden frequency among Polish subjects

Polosak J, Roszkowska-Gancarz M, Kurylowicz A, Owczarz M, Dobosz P, Mossakowska M, Szybinska A, Puzianowska-Kuznicka M

Decreased expression and the Lys751Gln polymorphism of the XPD gene are associated with extreme longevity

Czarnecka AM, Krawczyk T, Plak K, Klemba A, Zdrozny M, Arnold RS, Kofler B, Golik P, Szybinska A, Lubinski J, Mossakowska M, Bartnik E, Petros JA

Mitochondrial genotype and breast cancer predisposition

2009

Puzianowska-Kuznicka M, Kuznicki J.

The ER and ageing II: calcium homeostasis.

Blazejczyk M, Sobczak A, Debowska K, Wisniewska MB, Kirilenko A, Pikula S, Jaworski J, Kuznicki J, Wojda U.

Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2) in brain indicates its function in NMDA receptor mediated Ca2+ signaling.

Bojarski L, Pomorski P, Szybinska A, Drab M, Skibinska-Kijek A, Gruszczynska-Biegala J, Kuznicki J

Presenilin-dependent expression of STIM proteins and dysregulation of capacitative Ca2+ entry in familial Alzheimer's disease.

Skibinska-Kijek A, Wisniewska MB, Gruszczynska-Biegala J, Methner A, Kuznicki J

Immunolocalization of STIM1 in the mouse brain.

Klejman ME, Gruszczynska-Biegala J, Skibinska-Kijek A, Wisniewska MB, Misztal K, Blazejczyk M, Bojarski L, Kuznicki J.

Expression of STIM1 in brain and puncta-like co-localization of STIM1 and ORAI1 upon depletion of Ca(2+) store in neurons.

2008

Sbroggiò M, Ferretti R, Percivalle E, Gutkowska M, Zylicz A, Michowski W, Kuznicki J, Accornero F, Pacchioni B, Lanfranchi G, Hamm J, Turco E, Silengo L, Tarone G, Brancaccio M.

The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1.

Blazejczyk M, Wojda U

Factfile: Calmyrin 1 (review)

Filipek A, Michowski W, Kuznicki J

Involvement of S100A6 (calcyclin) and its binding partners in intracellular signaling pathways

Zabka M, Leśniak W, Prus W, Kuźnicki J, Filipek A

Sgt1 has co-chaperone properties and is up-regulated by heat shock.

Lanni C, Racchi M, Mazzini G, Ranzenigo A, Polotti R, Sinforiani E, Olivari L, Barcikowska M, Styczynska M, Kuznicki J, Szybinska A, Govoni S, Memo M, Uberti D

Conformationally altered p53: a novel Alzheimer's disease marker?

Wojda U, Salinska E, Kuznicki J

Calcium ions in neuronal degeneration

Bojarski L, Herms J, Kuznicki J

Calcium dysregulation in Alzheimer's disease

Mossakowska M, Barcikowska M, Broczek K, Grodzicki T, Klich-Raczka A, Kupisz-Urbanska M, Podsiadly-Marczykowska T, Sikora E, Szybinska A, Wieczorowska-Tobis K, Zyczkowska J, Kuznicki J

Polish Centenarians Programme. Multidisciplinary studies of successful ageing: aims, methods, and preliminary results

Winiarska M, Bil J, Wilczek E, Wilczynski GM, Lekka M, Engelberts PJ, Mackus WJ, Gorska E, Bojarski L, Stoklosa T, Nowis D, Kurzaj Z, Makowski M, Glodkowska E, Issat T, Mrowka P, Lasek W, Dabrowska-Iwanicka A, Basak GW, Wasik M, Warzocha K, Sinski M, Gaciong Z, Jakobisiak M, Parren PW, Golab J.

Statins impair antitumor effects of rituximab by inducing conformational changes of CD20.

Erickson RP, Larson-Thomé K, Weberg L, Szybinska A, Mossakowska M, Styczynska M, Barcikowska M, Kuznicki J

Variation in NPC1, the gene encoding Niemann-Pick C1, a protein involved in intracellular cholesterol transport, is associated with Alzheimer disease and/or aging in the Polish population

2007

Wollmer MA, Sleegers K, Ingelsson M, Zekanowski C, Brouwers N, Maruszak A, Brunner F, Huynh KD, Kilander L, Brundin RM, Hedlund M, Giedraitis V, Glaser A, Engelborghs S, De Deyn PP, Kapaki E, Tsolaki M, Daniilidou M, Molyva D, Paraskevas GP, Thal DR, Barcikowska M, Kuznicki J, Lannfelt L, Van Broeckhoven C, Nitsch RM, Hock C, Papassotiropoulos A.

Association study of cholesterol-related genes in Alzheimer's disease

Spiechowicz M, Zylicz A, Bieganowski P, Kuznicki J,Filipek A

Hsp70 is a new target of Sgt1-an interaction modulated by S100A6

Schneider G, Nieznanski K, Kilanczyk E,Bieganowski P, Kuznicki J, Filipek A

CacyBP/SIP interacts with tubulin in neuroblastoma NB2a cells and induces formation of globular tubulin assemblies

Leśniak W, Słomnicki ŁP, Kuźnicki J

Epigenetic control of the S100A6 (calcyclin) gene expression

Bojarski L, Lewandowicz A, Blazejczyk M, Sobczak A, Kuznicki J, Wojda U

Biochemical properties of endogenous presenilin 1 and presenilin 2 in cultured human B-lymphocytes

2006

Blazejczyk M, Wojda U, Sobczak A, Spilker C, Bernstein HG, Gundelfinger ED, Kreutz MR, Kuznicki J

Ca2+-independent binding and cellular expression profiles question a significant role of calmyrin in transduction of Ca2+-signals to Alzheimer's disease-related presenilin 2 in forebrain

Bik W, Baranowska-Bik A, Wolinska-Witort E, Martynska L, Chmielowska M, Szybinska A, Broczek K, Baranowska B

The relationship between adiponectin levels and metabolic status in centenarian, early elderly, young and obese women

Baranowska B, Bik W, Baranowska-Bik A, Wolinska-Witort E, Szybinska A, Martynska L, Chmielowska M

Neuroendocrine control of metabolic homeostasis in Polish centenarians

Spiechowicz M, Bernstein HG, Dobrowolny H, Leśniak W, Mawrin C, Bogerts B, Kuźnicki J, Filipek A

Density of Sgt1-immunopositive neurons is decreased in the cerebral cortex of Alzheimer's disease brain.

Leśniak W, Kuźnicki J

Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter.

Groves P, Palczewska M, Kuznicki J

Calretinin, an EF-hand calcium-binding proteins, binds zinc and Cooper

Zekanowski C, Golan MP, Krzyśko KA, Lipczyńska-Łojkowska W, Filipek S, Kowalska A, Rossa G, Pepłońska B, Styczyńska M, Maruszak A, Religa D, Wender M, Kulczycki J, Barcikowska M, Kuźnicki J.

Two novel presenilin 1 gene mutations connected with frontotemporal dementia-like clinical phenotype: genetic and bioinformatic assessment.

2005

Zekanowski C, Religa D, Safranow K, Maruszak A, Dziedziejko V, Styczyńska M, Gacia M, Golan M, Pepłońska B, Chlubek D, Kuźnicki J, Barcikowska M

The -22c/t polymorphism in presenilin 1 gene is not connected with late-onset and early-onset familial Alzheimer's disease in Poland

Sobczak A, Blazejczyk M, Piszczek G, Zhao G, Kuznicki J, Wojda U.

Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form.

Puzianowska-Kuznicka M, Kuznicki J.

Genetic alterations in accelerated ageing syndromes. Do they play a role in natural ageing?

Palczewska M, Batta G, Groves P, Linse S, Kuznicki J

Characterization of calretinin I-II as an EF-hand, Ca2+, H+-sensing domain

Leśniak W, Szczepańska A, Kuźnicki J.

Calcyclin (S100A6) expression is stimulated by agents evoking oxidative stress via the antioxidant response element.

Bhattacharya S, Lee YT, Michowski W, Jastrzebska B, Filipek A, Kuznicki J, Chazin WJ

The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies

Bernstein HG, Blazejczyk M, Rudka T, Gundelfinger ED, Dobrowolny H, Bogerts B, Kreutz MR, Kuznicki J, Wojda U

The Alzheimer disease-related calcium-binding protein Calmyrin is present in human forebrain with an altered distribution in Alzheimer's as compared to normal ageing brains

2004

Brzezińska A, Magalska A, Szybińska A, Sikora E

Proliferation and apoptosis of human CD8(+)CD28(+) and CD8(+)CD28(-) lymphocytes during aging

Zekanowski C, Religa D, Graff C, Filipek S, Kuźnicki J

Genetic aspects of Alzheimer's disease

Zekanowski C, Pepłońska B, Styczyńska M, Religa D, Pfeffer A, Czyzewski K, Gabryelewicz T, Szybińska A, Kijanowska-Haładyna B, Kotapka-Minc S, Łuczywek E, Barczak A, Wasiak B, Chodakowska-Zebrowska M, Przekop I, Kuźnicki J, Barcikowska M

The E318G substitution in PSEN1 gene is not connected with Alzheimer's disease in a large Polish cohort.

Wojda U.

Gene therapy of hematopoietic cells: development of strategies and vectors. (in Polish)

Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ.

Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.

Billing-Marczak K, Ziemińska E, Leśniak W, Łazarewicz JW, Kuźnicki J

Calretinin gene promoter activity is differently regulated in neurons and cancer cells. Role of AP2-like cis element and zinc ions

2003

Zekanowski C

Molecular diagnostics of Alzheimer's disease (in Polish)

Zekanowski C

The sun, the genome, the internet… eugenics?

Tighe O, Dunican D, O'Neill C, Bertorelle G, Beattie D, Graham C, Zschocke J, Cali F, Romano V, Hrabincova E, Kozak L, Nechyporenko M, Livshits L, Guldberg P, Jurkowska M, Zekanowski C, Perez B, Desviat LR, Ugarte M, Kucinskas V, Knappskog P, Treacy E, N

Genetic diversity within the R408W phenylketonuria mutation lineages in Europe

Zekanowski C, Przyluska-Fiszer A, Barcikowska M

Alzheimer's disease: between diagnostic, economy and ethic (in Polish)

Zekanowski C, Pepłońska B, Styczyńska M, Gustaw K, Kuźnicki J, Barcikowska M

Mutation screening of the MAPT and STH genes in Polish patients with clinically diagnosed frontotemporal dementia

Zekanowski C, Styczyńska M, Pepłońska B, Gabryelewicz T, Religa D, Ilkowski J, Kijanowska-Haładyna B, Kotapka-Minc S, Mikkelsen S, Pfeffer A, Barczak A, Łuczywek E, Wasiak B, Chodakowska-Zebrowska M, Gustaw K, Łaczkowski J, Sobów T, Kuźnicki J, Barcikowsk

Mutations in presenilin 1, presenilin 2 and amyloid precursor protein genes in patients with early-onset Alzheimer's disease in Poland

Pepłońska B, Zekanowski C, Religa D, Czyzewski K, Styczyńska M, Pfeffer A, Gabryelewicz T, Gołebiowski M, Luczywek E, Wasiak B, Barczak A, Chodakowska M, Barcikowska M, Kuźnicki J

Strong association between Saitohin gene polymorphism and tau haplotype in the Polish population

Palczewska M, Groves P, Batta G, Heise B, Kuźnicki J

Calretinin and calbindin D28k have different domain organizations

Nowotny M, Spiechowicz M, Jastrzebska B, Filipek A, Kitagawa K, Kuznicki J

Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins

2002

Billing-Marczak K, Buzanska L, Winsky L, Nowotny M, Rudka T, Isaacs K, Belin MF, Kuznicki J.

AP2-like cis element is required for calretinin gene promoter activity in cells of neuronal phenotype differentiated from multipotent human cell line DEV.

Filipek A, Jastrzebska B, Nowotny M, Kuznicki J

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family

Zekanowski C

Genetic aspects of Alzheimer’s disease

Zekanowski C, Gizewska M, Nowacka M, Cabalska B

Novel PKU treatment methods

Cabalska B, Nowacka M, Nowaczewska I, Zekanowski C, Zorska K

Atypical PKU - treatment effectiveness

Filipek A, Jastrzebska B, Nowotny M, Kwiatkowska K, Hetman M, Surmacz L, Wyroba E,Kuznicki J

Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells

Filipek A, Jastrzebska B, Nowotny M, Kuznicki J

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family

Billing-Marczak K, Buzanska L, Winsky L, Nowotny M, Rudka T, Isaacs K, Belin MF, Kuznicki J

AP2-like cis element is required for calretinin gene promoter activity in cells of neuronal phenotype differentiated from multipotent human cell line DEV

2001

Palczewska M, Groves P, Ambrus A, Kaleta A, Kövér KE, Batta G, Kuźnicki J.

Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93)

2000

Leśniak W, Swart GW, Bloemers HP, Kuźnicki J.

Regulation of cell specific expression of calcyclin (S100A6) in nerve cells and other tissues.

Leśniak W, Jezierska A, Kuźnicki J.

Upstream stimulatory factor is involved in the regulation of the human calcyclin (S100A6) gene.

Jastrzebska B, Filipek A, Nowicka D, Kaczmarek L, Kuznicki J.

Calcyclin (S100A6) binding protein (CacyBP) is highly expressed in brain neurons.

Nowotny M, Bhattacharya S, Filipek A, Krezel AM, Chazin W, Kuznicki J.

Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein.

Mossakowska M, Puzianowska-Kuznicka M, Barcikowska M, Chiron-Jouan S, Czyzewski K, Derejczyk J, Franceschi C, Gabryelewicz T, Galus K, Grodzicki T, Gross R, Klich-Raczka A, Luczywek E, Passeri G, Pfeffer A, Pruszynski J, Radziszewska E, Sikora E, Sosnowski M, Styczynska M, Wasiak B, Wieczorowska-Tobis K, Zyczkowska J, Kuznicki J

Polish Centenarians Program “PolStu99” – search for longevity promoting factors (in Polish)

Zekanowski C

Cloning - the crossroads of biology and culture