Preprint Phosphorylation of the AP2 μ2 subunit by p70S6 kinase is needed for clathrin-mediated endocytosis. Tempes A, Brzozowska A, Węgierski T, Fasemire A, Olek K, Jastrzębski K, Ewa Liszewska E, Misztal K, Machnicka K, Macias M, Szybińska A, Sitkiewicz E, Malinowska A, Gozdz A, Wyszyńska A, Łasica A, Hoffmann-Młodzianowska M, Miączyńska M, Górna MW, Pokrzywa W, Jaworski J. Malik AR. bioRxiv. 2026 Preprint Iron deficiency drives metabolic adaptation of red pulp macrophages via FPN-SYK signaling and BCAA catabolism to enhance erythrophagocytosis Mandal PK, Mahadeva R, Chouhan K, Slusarczyk P,
Zurawska G, Niklewicz M, Macias M, Szybińska A, Jończy A, Liu Z, Ginhoux F, Lenartowicz M, Pokrzywa W, Nemeth E, Mleczko-Sanecka K. bioRxiv. 2026 Publication Volatile and non-volatile pathogen cues shape host extracellular vesicles production in pre-infection response. Kołodziejska K, Szczepańska A, Vadlamani S, Ponath Sukumaran R, Radkiewicz M, Bringmann H, Pujol N, Pokrzywa W, Turek M. Nat Commun. 2025 Preprint Mitochondrial redox homeostasis links organellar stress surveillance to germline and somatic integrity in Caenorhabditis elegans. Valenzuela-Villatoro M, de la Cruz-Ruiz P, Guerrero-Gomez D, Gomez-Orte E, Schiavi A, Maglioni S, Montero M, Fonteriz R, Casas-Martinez JC, Briand N, Xu J, Rodriguez-Palero MJ, Artal-Sanz M, Olek K, Polaczyk J, Turek M, Pokrzywa W, Xu S, Irazoqui J, McDonagh B, Alvarez J, Olmedo M, Ventura N, Cabello J, Miranda-Vizuete A. bioRxiv . 2025 Preprint A dosage-sensitive ALLO-1 network coordinates mitochondrial quality control to enable functional recovery of structurally compromised muscle. Sarkar A, Biriczova L, Szulc NA, Thapa P, Serwa R, Pokrzywa W. bioRxiv. 2025 Preprint CHIP, VCP, and Nucleolar Gatekeepers Remodel the Nucleolus into a Stress-Responsive Proteostasis Hub. Piechota M, Biriczova L, Kowalski K, Garcia FA, Lesniczak-Staszak M, Szulc NA, Szaflarski W, Sawarkar R, Pokrzywa W. bioRxiv. 2025 Preprint Nutritional iron deficiency elicits profound rewiring of red pulp macrophage functions via high FPN and SYK-mediated signaling. Mandal PK, Chouhan K, Slusarczyk P, Mahadeva R, Zurawska G, Niklewicz M, Jończy A, Lenartowicz M, Pokrzywa W, Meynard D, Nemeth E, Mleczko-Sanecka K. bioRxiv. 2025 Publication Tissue-specific consequences of tag fusions on protein expression in transgenic mice. Taylor GCA, Macdonald L, Szulc NA, Gudauskaite E, Hernandez Moran B, Brisbane JM, Donald M, Taylor E, Zheng D, Gu B, Mill P, Yeyati PL, Pokrzywa W, Ribeiro de Almeida C, Wood AJ. PLoS Genet. 2025 Publication Quantitative insights into protein turnover and ubiquitination with HiBiT and NanoBRET. Piechota M, Pokrzywa W. Methods in Enzymology. 2025 Publication DEGRONOPEDIA: A practical guide to identifying and targeting protein degrons. Szulc NA, Pokrzywa W. Methods in Enzymology. 2025 Preprint Tissue-specific consequences of tag fusions on protein expression in transgenic mice. Taylor GCA, Macdonald L, Szulc NA, Gudauskaite E,
Moran BH, Brisbane J, Donald M, Taylor E, Zheng D, Gu B, Mill P, Yeyati PL, Pokrzywa W, Ribeiro de Almeida C, Wood AJ. bioRxiv. 2025 Preprint p70S6 kinase-dependent phosphorylation of the μ2 subunit of the AP2 adaptor complex is needed for clathrin-mediated endocytosis. Tempes A, Brzozowska A, Węgierski T, Olek K, Jastrzębski K, Liszewska E, Misztal K, Machnicka K, Macias M, Szybińska A, Sitkiewicz E, Gozdz A, Wróbel AG, Miaczynska M, Pokrzywa W, Jaworski J, Malik AR. bioRxiv. 2025 Preprint A novel MAPK1 variant in a family with complex psychiatric and neurodegenerative clinical phenotype. Dubey AA, Rydzanicz M, Barczak A, Szulc NA, Surpeta B, Kuzniewska B, Gaweda-Walerych K, Wężyk M, Berdyński M, Fichna JP, Kostrzewa G, Gasperowicz P, Stępniak I, Szymańska K, Szczałuba K, Brezovsky J, Dziembowska M, Pokrzywa W, Ploski R. Research Square. 2025 Publication DEGRADATOR: A Gaming Expedition Into Targeted Protein Degradation Therapies. Szulc NA, Olchowik A, Jaszczak P, Janiak B, Cup M, Tomaszewski J, Pokrzywa W. J Chem Educ. 2025 Publication HSP70 inhibits CHIP E3 ligase activity to maintain germline function in Caenorhabditis elegans. Thapa P, Chikale RV, Szulc NA, Pandrea MT, Sztyler A, Jaggi K, Niklewicz M, Serwa RA, Hoppe T, Pokrzywa W. J Biol Chem. 2024 Publication Optogenetic induction of mechanical muscle stress identifies myosin regulatory ubiquitin ligase NHL-1 in C. elegans. Kutzner CE, Bauer KC, Lackmann JW, Acton RJ, Sarkar A, Pokrzywa W, Hoppe T. Nat Commun. 2024 Publication Floxuridine supports UPS independent of germline signaling and proteostasis regulators via involvement of detoxification in C. elegans. Dubey AA, Sarkar A, Milcz K, Szulc NA, Thapa P, Piechota M, Serwa RA, Pokrzywa W. PLoS Genet. 2024 Publication SARS-CoV-2 inhibitory potential of fish oil-derived 2-pyrone compounds by acquiring linoleic acid binding site on the spike protein. Duragkar N, Chikhale R, Piechota M, Danta CC, Gandhale P, Itankar P, Chikhale S, Gurav N, Khan MS, Pokrzywa W, Thapa P, Bryce R, Gurav S. Int J Biol Macromol. 2024 Publication Pheromone-based communication influences the production of somatic extracellular vesicles in C. elegans Szczepańska A, Olek K, Kołodziejska K, Yu J, Ibrahim AT, Adamkiewicz L, Schroeder FC, Pokrzywa W, Turek M. Nat Commun. 2024 Publication DEGRONOPEDIA: a web server for proteome-wide inspection of degrons. Szulc NA, Stefaniak F, Piechota M, Soszyńska A, Piórkowska G, Cappannini A, Bujnicki JM, Maniaci C, Pokrzywa W. Nucleic Acids Res. 2024 Publication SAM, SAH and C. elegans longevity: insights from a partial AHCY deficiency model. Thapa P, Olek K, Kowalska A, Serwa RA, Pokrzywa W. NPJ Aging. 2023 Preprint Sterility-Independent Enhancement of Proteasome Function via Floxuridine-Triggered Detoxification in C. elegans. Dubey AA, Szulc NA, Piechota M, Serwa RA, Pokrzywa W. bioRxiv. 2023 Publication Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasis. Szulc NA, Piechota M, Biriczova L, Thapa P, Pokrzywa W. iScience. 2023 Publication In silico analysis of the profilaggrin sequence indicates alterations in the stability, degradation route, and intracellular protein fate in filaggrin null mutation carriers. Paul AA, Szulc NA, Kobiela A, Brown SJ, Pokrzywa W, Gutowska-Owsiak D. Front. Mol. Biosci.. 2023 Publication Impaired iron recycling from erythrocytes is an early hallmark of aging. Slusarczyk P, Mandal PK, Zurawska G, Niklewicz M, Chouhan K, Mahadeva R, Jończy A, Macias M, Szybinska A, Cybulska-Lubak M, Krawczyk O, Herman S, Mikula M, Serwa R, Lenartowicz M, Pokrzywa W, Mleczko-Sanecka K. eLife. 2023 Preprint Lysine-deficient proteome can be regulated through non-canonical ubiquitination and ubiquitin-independent proteasomal degradation. Szulc N,  Piechota M, Thapa P, Pokrzywa W. bioRxiv. 2023 Publication Preparation of Caenorhabditis elegans for Scoring of Muscle-derived Exophers. Banasiak K, Turek M, Pokrzywa W. bio-protocol Journal. 2023 Publication A novel de novo FEM1C variant is linked to neurodevelopmental disorder with absent speech, pyramidal signs, and limb ataxia. Dubey AA, Krygier M, Szulc NA, Rutkowska K, Kosińska J, Pollak A, Rydzanicz M, Kmieć T, Mazurkiewicz-Bełdzińska M, Pokrzywa W, Płoski R. Hum Mol Genet.. 2023 Preprint Pheromone-dependent olfaction bidirectionally regulates muscle extracellular vesicles formation. Banasiak K, Szczepańska A, Kołodziejska K, Ibrahim AT, Pokrzywa W, Turek M. bioRxiv. 2022 Publication A Dimer-Monomer Switch Controls CHIP-Dependent Substrate Ubiquitylation and Processing. Balaji V, Müller L, Lorenz R, Kevei É, Zhang WH, Santiago U, Gebauer J, Llamas E, Vilchez D, Camacho CJ, Pokrzywa W, Hoppe T. Mol Cell. 2022